Shifting Control Algorithm for a Single-Axle Parallel Plug-In Hybrid Electric Bus Equipped with EMT

Combining the characteristics of motor with fast response speed, an electric-drive automated mechanical transmission (EMT) is proposed as a novel type of transmission in this paper. Replacing the friction synchronization shifting of automated manual transmission (AMT) in HEVs, the EMT can achieve active synchronization of speed shifting. The dynamic model of a single-axle parallel PHEV equipped with the EMT is built up, and the dynamic properties of the gearshift process are also described. In addition, the control algorithm is developed to improve the shifting quality of the PHEV equipped with the EMT in all its evaluation indexes. The key techniques of changing the driving force gradient in preshifting and shifting compensation phases as well as of predicting the meshing speed in the gear meshing phase are also proposed. Results of simulation, bench test, and real road test demonstrate that the proposed control algorithm can reduce the gearshift jerk and the power interruption time noticeably

Germline PTPRD mutations in Ewing sarcoma: biologic and clinical implications.

Ewing sarcoma occurs in children, adolescents and young adults. High STAT3 levels have been reported in approximately 50% of patients with Ewing sarcoma, and may be important in tumorigenesis. Protein tyrosine phosphatase delta (PTPRD) is a tumor suppressor that inhibits STAT3 activation. To date, while somatic mutations in PTPRD have been reported in diverse tumors, germline mutations of PTPRD have not been investigated in Ewing sarcoma or other cancers. We identified a novel germline mutation in the PTPRD gene in three of eight patients (37.5%) with metastatic Ewing sarcoma. Although the functional impact in two of the patients is unclear, in one of them the aberration was annotated as a W775stop germline mutation, and would be expected to lead to gene truncation and, hence, loss of the STAT3 dephosphorylation function of PTPRD. Since STAT3 is phosphorylated after being recruited to the insulin growth factor receptor (IGF-1R), suppression of IGF-1R could attenuate the enhanced STAT3 activation expected in the presence of PTPRD mutations. Of interest, two of three patients with germline PTPRD mutations achieved durable complete responses following treatment with IGF-1R monoclonal antibody-based therapies. Our pilot data suggest that PTPRD germline mutations may play a role in the development of Ewing sarcoma, a disease of young people, and their presence may have implications for therapy

Exploring the differential stages of the pigment metabolism by pre-harvest bagging and post-harvest ethylene de-greening of Eureka lemon peel

Pre-harvest bagging or post-harvest ethylene treatments on lemons are commonly applied to change the surface color from green to favorable yellow. In this study, the differential mechanisms of the pigment metabolism by the two treatments were investigated by pigments contents and related genetic expression. The results showed that both treatments reduced the number of chloroplasts and the content of chlorophyll. The differential expression of PSY1 and PSY2 were observed, causing the different accumulation of the main carotenoid phytoene content. The differential expression of NYC resulted in altered contents of chlorophyll a and chlorophyll b, and further led to the difference in a* value. More interestingly, the degradation of chlorophyll uncovered the color of carotenoids, leading to the color changed from green to yellow

Hydrogen peroxide and glucose concentration measurement using optical fiber grating sensors with corrodible plasmonic nanocoatings

We propose and demonstrate hydrogen peroxide (H2O2) and glucose concentration measurements using a plasmonic optical fiber sensor. The sensor utilizes a tilted fiber Bragg grating (TFBG) written in standard single mode communication fiber. The fiber is over coated with an nm-scale film of silver that supports surface plasmon resonances (SPRs). Such a tilted grating SPR structure provides a high density of narrow spectral resonances (Q-factor about 105) that overlap with the broader absorption band of the surface plasmon waves in the silver film, thereby providing an accurate tool to measure small shifts of the plasmon resonance frequencies. The H2O2 to be detected acts as an oxidant to etch the silver film, which has the effect of gradually decreasing the SPR attenuation. The etching rate of the silver film shows a clear relationship with the H2O2 concentration so that monitoring the progressively increasing attenuation of a selected surface plasmon resonance over a few minutes enables us to measure the H2O2 concentration with a limit of detection of 0.2 µM. Furthermore, the proposed method can be applied to the determination of glucose in human serum for a concentration range from 0 to 12 mM (within the physiological range of 3-8 mM) by monitoring the H2O2 produced by an enzymatic oxidation process. The sensor does not require accurate temperature control because of the inherent temperature insensitivity of TFBG devices referenced to the core mode resonance. A gold mirror coated on the fiber allows the sensor to work in reflection, which will facilitate the integration of the sensor with a hypodermic needle for in vitro measurements. The present study shows that Ag-coated TFBG-SPR can be applied as a promising type of sensing probe for optical detection of H2O2 and glucose detection in human serum

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Design and analysis of low-power and high-speed Manchester carry-bypass adders

This project pays more attention to low power Manchester carry-bypass adders. compared with the ripple carry adder and Carry-select adder, power consumption of Manchester adder under 1V supply voltage are improved by 47.1% and 36.97% respectively. Two ways of improving Manchester adder performance are proposed . Reducing Vdd is a good way to reduce power consumption as long as the circuit speed meets requirement. When the supply voltage drops from 1.2V to 0.8V, the power consumption is reduced by 58.73%. Using double carry chain is the other effective way. For 8-bit Manchester adder , double carry chain structure have 55.64% less worst case delay time than conventional Manchester carry-bypass structure under the condition of 1V supply voltage, however, the power consumption of double carry chain is higher than conventional Manchester adder. All the simulations were done in Cadence environment using TSMC 40nm CMOS technology.Master of Science (Electronics

Effects of Vertical Water Mass Segregation on Bacterial Community Structure in the Beaufort Sea

The Arctic Ocean is one of the least well-studied marine microbial ecosystems. Its low-temperature and low-salinity conditions are expected to result in distinct bacterial communities, in comparison to lower latitude oceans. However, this is an ocean currently in flux, with climate change exerting pronounced effects on sea-ice coverage and freshwater inputs. How such changes will affect this ecosystem are poorly constrained. In this study, we characterized the bacterial community compositions at different depths in both coastal, freshwater-influenced, and pelagic, sea-ice-covered locations in the Beaufort Sea in the western Canadian Arctic Ocean. The environmental factors controlling the bacterial community composition and diversity were investigated. Alphaproteobacteria dominated the bacterial communities in samples from all depths and stations. The Pelagibacterales and Rhodobacterales groups were the predominant taxonomic representatives within the Alphaproteobacteria. Bacterial communities in coastal and offshore samples differed significantly, and vertical water mass segregation was the controlling factor of community composition among the offshore samples, regardless of the taxonomic level considered. These data provide an important baseline view of the bacterial community in this ocean system that will be of value for future studies investigating possible changes in the Arctic Ocean in response to global change and/or anthropogenic disturbance

Disappearing and appearing: Temporal binding effects are consistent across situations

Sense of agency refers to the feeling of control over actions and action outcomes. Previous studies were mostly confined to the situation of performing actions to make objects appear, while it remains unexplored whether we experience sense of agency when making objects disappear. Here, we examined the temporal binding effect, an implicit index of sense of agency, in performing actions to make objects disappear and compared the magnitude of this effect in the appearing and disappearing situations. Results showed that the temporal binding effect emerged when object&#39;s disappearances served as action outcomes. Moreover, the temporal binding effects in the appearing and disappearing situations did not differ significantly. Our findings extend the temporal binding effect to the situation of voluntarily making objects disappear, suggesting a comparable level of implicit sense of agency when voluntarily making objects disappear and appear.</p

Water Quality Prediction Method Based on IGRA and LSTM

Water quality prediction has great significance for water environment protection. A water quality prediction method based on the Improved Grey Relational Analysis (IGRA) algorithm and a Long-Short Term Memory (LSTM) neural network is proposed in this paper. Firstly, considering the multivariate correlation of water quality information, IGRA, in terms of similarity and proximity, is proposed to make feature selection for water quality information. Secondly, considering the time sequence of water quality information, the water quality prediction model based on LSTM, whose inputs are the features obtained by IGRA, is established. Finally, the proposed method is applied in two actual water quality datasets: Tai Lake and Victoria Bay. Experimental results demonstrate that the proposed method can take full advantage of the multivariate correlations and time sequence of water quality information to achieve better performance on water quality prediction compared with the single feature or non-sequential prediction methods

Study on Solidification and Stabilization of Antimony-Containing Tailings with Metallurgical Slag-Based Binders

Blast furnace slag (BFS), steel slag (SS), and flue gas desulfurized gypsum (FGDG) were used to prepare metallurgical slag-based binder (MSB), which was afterwards mixed with high-antimony-containing mine tailings to form green mining fill samples (MBTs) for Sb solidification/stabilization (S/S). Results showed that all MBT samples met the requirement for mining backfills. In particular, the unconfined compressive strength of MBTs increased with the curing time, exceeding that of ordinary Portland cement (OPC). Moreover, MBTs exhibited the better antimony solidifying properties, and their immobilization efficiency could reach 99%, as compared to that of OPC. KSb(OH)6 was used to prepare pure MSB paste for solidifying mechanism analysis. Characteristics of metallurgical slag-based binder (MSB) solidified/stabilized antimony (Sb) were investigated via X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). According to the results, the main hydration products of MSB were C-S-H gel and ettringite. Among them, C-S-H gel had an obvious adsorption and physical sealing effect on Sb, and the incorporation of Sb would reduce the degree of C-S-H gel polymerization. Besides, ettringite was found to exert little impact on the solidification and stabilization of Sb. However, due to the complex composition of MSB, it was hard to conclude whether Sb entered the ettringite lattice