Multipurpose watermarking approach for copyright and integrity of steganographic autoencoder models

With the great achievements of deep learning technology, neural network models have emerged as a new type of intellectual property. Neural network models’ design and training require considerable computational resources and time. Watermarking is a potential solution for achieving copyright protection and integrity of neural network models without excessively compromising the models’ accuracy and stability. In this work, we develop a multipurpose watermarking method for securing the copyright and integrity of a steganographic autoencoder referred to as “HiDDen.” This autoencoder model is used to hide different kinds of watermark messages in digital images. Copyright information is embedded with imperceptibly modified model parameters, and integrity is verified by embedding the Hash value generated from the model parameters. Experimental results show that the proposed multipurpose watermarking method can reliably identify copyright ownership and localize tampered parts of the model parameters. Furthermore, the accuracy and robustness of the autoencoder model are perfectly preserved

Mechanism Underlying Defective Interferon Gamma-Induced IDO Expression in Non-obese Diabetic Mouse Fibroblasts

Indoleamine 2,3-dioxygenase (IDO) can locally suppress T cell-mediated immune responses. It has been shown that defective self-tolerance in early prediabetic female non-obese diabetic (NOD) mice can be attributed to the impaired interferon-gamma (IFN-γ)- induced IDO expression in dendritic cells of these animals. As IFN-γ can induce IDO in both dendritic cells and fibroblasts, we asked the question of whether there exists a similar defect in IFN-γ-induced IDO expression in NOD mice dermal fibroblasts. To this end, we examined the effect of IFN-γ on expression of IDO and its enzymatic activity in NOD dermal fibroblasts. The results showed that fibroblasts from either prediabetic (8 wks of age) female or male, and diabetic female or male (12 and 24 wks of age respectively) NOD mice failed to express IDO in response to IFN-γ treatment. To find underlying mechanisms, we scrutinized the IFN- γ signaling pathway and investigated expression of other IFN-γ-modulated factors including major histocompatibility complex class I (MHC-I) and type I collagen (COL-I). The findings revealed a defect of signal transducer and activator of transcription 1 (STAT1) phosphorylation in NOD cells relative to that of controls. Furthermore, we found an increase in MHC-I and suppression of COL-I expression in fibroblasts from both NOD and control mice following IFN-γ treatment; indicating that the impaired response to IFN-γ in NOD fibroblasts is specific to IDO gene. Finally, we showed that an IFN-γ-independent IDO expression pathway i.e. lipopolysaccharide (LPS)-mediated-c-Jun kinase is operative in NOD mice fibroblast. In conclusion, the findings of this study for the first time indicate that IFN-γ fails to induce IDO expression in NOD dermal fibroblasts; this may partially be due to defective STAT1 phosphorylation in IFN-γ-induced-IDO signaling pathway

Fibroblast cell-based therapy prevents induction of alopecia areata in an experimental model

YesAlopecia areata (AA) is an autoimmune hair loss disease with infiltration of proinflammatory cells into hair follicles. Current therapeutic regimens are unsatisfactory mainly because of the potential for side effects and/or limited efficacy. Here we report that cultured, transduced fibroblasts, which express the immunomodulatory molecule indoleamine 2,3-dioxygenase (IDO), can be applied to prevent hair loss in an experimental AA model. A single intraperitoneal (IP) injection of IDO-expressing primary dermal fibroblasts was given to C3H/HeJ mice at the time of AA induction. While 60–70% of mice that received either control fibroblasts or vehicle injections developed extensive AA, none of the IDO-expressing fibroblast-treated mice showed new hair loss up to 20 weeks post injection. IDO cell therapy significantly reduced infiltration of CD4+ and CD8+ T cells into hair follicles and resulted in decreased expression of TNF-α, IFN-γ and IL-17 in the skin. Skin draining lymph nodes of IDO fibroblast-treated mice were significantly smaller, with more CD4+ CD25+ FoxP3+ regulatory T cells and fewer Th17 cells than those of control fibroblast and vehicle-injected mice. These findings indicate that IP injected IDO-expressing dermal fibroblasts can control inflammation and thereby prevent AA hair loss.Canadian Institutes of Health Researches (Funding Reference Number: 134214 and 136945)

Evaluation and Optimization of Refuge Green Space in the Central Area of Tianjin for Geological Disasters

Refuge Green Space (RGS) plays roles in both rapid evacuation and short-term resettlement in response to sudden natural hazards, especially geological disasters. As a result, its evaluation and location optimization are crucial for the development of sustainable cities. In this study, the Three-Step Floating Catchment Area Method (3SFCA) and Moran’s I index were used as measurement models. The rapid evacuation and short-term resettlement capacity of RGS were evaluated by calculating accessibility with 5, 10, 15 and 30 min of walking. The purpose of this study was to quantitatively evaluate the supply capacity of RGS in the central area of Tianjin and to provide recommendations for future additions to RGS. The results show that over 80% of the research area exhibited relatively poor service capacity within a 5–10 min walking distance, while less than 20% exhibited relatively good service capacity. Within a 15–30 min walking distance, approximately 60% of the study area was not well served by the RGS, while approximately 40% was well served. In conclusion, the layout of RGS in the central area of Tianjin exhibits unevenness and polarization of phenomena, and the supply of different categories of RGS varies widely. There is an insufficient supply of Emergency Sheltered Green Space and Temporary Sheltered Green Space, and that which is available is difficult to reach within 5–10 min. The coverage capacity of Disaster Prevention Park Space is relatively good, essentially covering the entire study area within a 15–30 min walking distance; however, the service capacity is average. Based on these conclusions, areas in which the RGS supply was weak were identified, and future potential RGS additions were identified in conjunction with the Green Space System Plan

Roles of ubiquitination-mediated protein degradation in plant responses to abiotic stresses

Ubiquitination is a major modifier of signaling in all eukaryotes that results in the conjugation of ubiquitin to the lysine residues of acceptor proteins. The targeted protein is then subjected to degradation by the 26S proteasome, the major protein degradation system in eukaryotes. The ubiquitin-proteasome system (UPS) greatly influences plant growth and development by modulating the activity, localization, and stability of proteins. Plants are frequently exposed to various abiotic stresses during their life cycles; they rely on proteomic plasticity achieved by the UPS to adapt to unfavorable environmental conditions. In stress signal pathways, a large number of components are modified by specific ubiquitination machinery. In this review, we highlight recent advances in understanding the roles of ubiquitination in plant responses to abiotic stresses, including salt and drought, temperature, ultraviolet (UV), and nutrient availability. The review focuses primarily on the roles of the UPS. In salt and/or drought stress signaling, a number of E3 ligases mediate the stress response in both abscisic acid (ABA)-dependent and ABA-independant pathways. The UPS-mediated regulation of several key ABA-regulated transcriptional factors, e.g. ABI3 and ABI5, has been well documented. In cold signaling, the transcription factor ICE1 is targeted by E3 ligase HOSI for proteosomal degradation. Under UV stress, CUL4-DDB1A-DDB2 E3 ligase participates in DNA excision repair, and COP1 interacts with the UVR8 mediated UV response. The UPS is also involved in the uptake, transport, and homeostasis of nutrients such as iron, phosphorus, and nitrogen. SIZ1-mediated sumoylation, a ubiquitin-like modification, is necessary for a number of processes involved in plant responses to abiotic stresses. A challenge moving forward for researchers is to define more UPS components and to characterize their functions in plant responses to stress conditions; there is particular interest in identifying the ubiquitination targets that function in specific stress signaling pathways. (C) 2014 Elsevier B.V. All rights reserved