The enhancement apoptosis of osteosarcoma mesenchymal stem cells co-cultivation with peripheral blood mononuclear cells sensitized by secretome and granulocyte macrophage colony-stimulating factor

The advanced, metastasis, and reccurent of osteosarcoma (OS) patients have a poor prognosis postaggresive surgery and chemotherapy. Peripheral blood mononuclear cells (PBMCs) as cell-based immunotherapy may successful in the OS treatment. To investigate the enhancement apoptosis of OS-mesenchymal stem cells (OS-MSCs) co-cultivated with PBMCs sensitized using the secretome and granulocyte macrophage colony-stimulating factor (GMCSF). This true experimental study with posttest only control group design and in vitro study. The sample was cultured OS-MSCs which confirmed by Cluster of Differentiation-133 using immunocytochemistry (ICC) and histopathology analysis. The sample divided into six groups accordingly: OS-MSC, OS-MSC + PMBC, OS-MSC + PMBC + Secretome, OS-MSC + PMBC + GMCSF, OS-MSC + PBMC + Secretome + GMCSF (n = 5/N = 30). The enhancement of OS-MSCs apoptosis was analyzed through Interleukin-2 (IL-2) level through the Enyzme-Linked Immunosorbent Assay examination, expression of Signal Transducers and Activators of Transcription (STAT)-3 and caspase-3 by ICC. One-way analysis of variance test and Tukey Honestly Significant Difference to analyze the difference between the groups (P 0.05). The co-cultivation of OS-MSCs and PBMSCs activated using secretome and GMCSF has a great ability to enhance OS-MSCs apoptosis

SPINAL K-WIRE MIGRATION FOLLOWING ACROMIOCLAVICULAR DISLOCATION SURGICAL TREATMENT: A CASE REPORT WITH SCOPING REVIEW DISCUSSION

Background: Kirschner wire as internal fixation is a common instrument used in any small bone, including on Acromioclavicular Dislocation Surgical Treatment (ADST). Due to the characteristic of the wire, which is less rigid than any other instrument, the wire can sometimes migrate unexpectedly into various locations. Wire migration has been reported sporadically since 1943. However, spinal migration is still underreported. In addition, the mechanism of spinal migration and the evacuation method remains unclear. Objective: The purpose of this study was to report a case of K-wire spinal migration to cervical vertebrae C6-C7 following surgical treatment and discuss the case using the results of a scoping review. Case: A 51 years-old man with wire migration to cervical vertebrae C6-C7 complained of painful sensations around the left side of the neck and left shoulder, which aggravated with shoulder movement. He underwent left acra omioclavicular joint repair surgery three years ago after a motorcycle accident. No other abnormalities were found in either physical or neurological examinations. Discussion: In the last 10 years, six cases of spinal migration after ADST have been reported, with less than one case reported a year. The cases' gender was all male, with a mean age of 43.33 years old. The majority of them underwent acromioclavicular fixation in 50% of cases, and the average time from migration to surgery is 71 months. The most common migration is located in the cervical region (66.67%), and the penetrating points are equal in all cases. In the non-bent wire group, an entire migration has been reported. The evacuation technique used in all previous cases was direct access surgery by gently pulling out the wire along its axis under visual control. Various mechanisms contribute to spinal migration, but shoulder movement is arguably the most influential factor. Conclusion: Cervical migration post-ADST commonly occurs and may cause severe morbidity

Biological activities and extraction technologies of Pheonix dactylifera: a review.

Date palm (Pheonix dactylifera L.) has long been known for its medical benefits. In this review, the therapeutic properties of P. dactylifera are presented in light of the analysis of huge past reviews that reported the bioactivities of both flesh and seed of this fruit. The reported bioactivities of P. dactylifera included antioxidants, anti-cancer, aphrodisiac, antimicrobial, and anti-diabetic properties. This review additionally highlights the extraction technologies (Soxhlet, maceration, heat under reflux, supercritical fluid extraction (SFE) and microwave assisted extraction) that were utilized as part of the extraction of P. dactylifera. This is because the quality and amount of the extraction yield depend on the extraction technology used. Accordingly, this review aims for underlining the potentials of P. dactylifera by compiling available data on the bioactivities and extraction technologies used to set the directions for the improvement of future research of this fruit

Pendekatan Sistematis Diagnosis, Terapi dan Follow-Up Tumor Muskuloskeletal (Multidisplinary Approach)

Insiden tumor muskuloskeletal rendah, sedangkan terapinya bisa sangat berat bagi pasien dan keluarganya, seperti tindakan amputasi tungkai. Dengan penanganan yang sempurna sekalipun, umur harapan hidup beberapa penderitanya tidak mencapai angka yang tinggi. Oleh sebab itu, di bidang tumor muskuloskeletal, mutlak dibutuhkan standar pelayanan yang tinggi mulai dari skrining, diagnosis, penatalaksanaan dan evaluasi pasca terapi. Salah satu standar pelayanan yang tinggi di bidang diagnosis adalah prinsip multidisiplin dalam penegakan diagnosis. Paling tidak dokter-dokter spesialis orthopedi, patologi dan radiologi bersama-sama membahas informasi dari masing-masing bidang untuk disatukan menjadi diagnosis yang tepat

Morselized bone autograft for high placement of acetabular component closure with hip arthroplasty revision after 3-years screws and cup loosening: A case report

Abstract Introduction and importance Aseptic loosening in high placement of the acetabular component seriously affects the hip and femoral head loads. Surgery revision is highly recommended with defect closure in previous place. Case presentation A-40-year-old man came with chief complaint of right groin pain and noticeable leg length discrepancy gait. The first hip arthroplasty through pseudo-acetabulum cup was done three years ago after neglected femoral head necrosis due to eight years of unknown hip dislocation in vehicle accident. On hip x-ray there is a screws and cup loosening, without any sign of infection from blood or from soft tissue which undergoes pathological and mold examination. The patient than assessed with periprosthetic aseptic loosening of hip dextra and simple total hip arthroplasty revision using true acetabulum location was done. The pseudo-acetabulum area closed with morselized bone autograft. One weeks after surgery, the wound healed properly. Hence, the patient sent to the rehabilitation. Clinical discussion Hip arthroplasty revision of aseptic loosening in high placement acetabular component should perform by returning to anatomical acetabular position. Cancellous morselized bone autograft (MBA) was used to closed the defect formed by previous procedure. Conclusion Revision of hip arthroplasty combined with morselized bone autograft can be considered for high placement acetabular component defect closure to provide better stability and strength in weight loads transfer

Talipes Equinovarus (TEV) and VACTERL Associated Anomalies: Narrative Literature Review

Clubfoot or known as Talipes Equinovarus (TEV), is a common anomaly in world population of newborn. This condition was reported in many studies as isolated anomaly but may come with other associated congenital anomalies. This review aims to further discuss the classification of TEV, its etiopathogenesis and how to diagnosis with all kind of VACTERL associated anomaly together with TEV. Many studies show a range of incidence between 1.1-4.5 per 1000 live birth per year and there is a chance of its condition followed by multiple congenital anomalies. Even though, this anomaly was an idiopathic condition, but still there is multifactorial etiology for its in which revealed through many studies also. Nevertheless, the orthopedic management of TEV with or without other congenital conditions still continue to develop to make a better improvement for the patients

Implantation of bovine hydroxyapatite and secretome with different oxygen concentration may improve massive bone defect regeneration: An experimental study on animal model Show less

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The Escalation of Osteosarcoma Stem Cells Apoptosis After the Co-Cultivation of Peripheral Blood Mononuclear Cells Sensitized with Mesenchymal Stem Cells Secretome and Colony Stimulating Factor-2 in vitro

Introduction: Peripheral blood mononuclear cells (PBMCs) sensitized with mesenchymal stem cells (MSCs) secretome and/or colony stimulating factor-2 (CSF-2) as an immunotherapy candidate may escalate osteosarcoma stem cells (OS-SCs) apoptosis. This study aimed to investigate the escalation of osteosarcoma stem cells’ apoptosis after the co-cultivation with PBMCs sensitized by MSCs secretome with/or CSF-2 and it was completed by analyzing the level of serum tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) and tumor necrosis factor-α (TNF-α) level, annexin V binding, caspase-3 and caspase-8 expression in vitro. Methods: OS-SCs were derived from a single human osteosarcoma sample with its high grade and osteoblastic essential clinical characteristics obtained from a biopsy before the chemotherapy treatment. They were then isolated and cultured confirmed by the cluster of differentiation-133 (FITC) by applying immunofluorescence analysis with fluorescein isothiocyanate (FITC) labeled. MSCs secretome was obtained with cells extracted from the bone marrow of a healthy patient. Furthermore, enzyme linked immunosorbent assay (ELISA) was utilized to analyze sTRAIL and TNF-α level in each group. The expression of caspase-3, caspase-8, and annexin V assay in each group was examined by applying the immunofluorescence labeled with FITC. The comparison analysis between treatment groups and the control group was performed by utilizing the analysis of variance (ANOVA) and continued with Tukey Honest Significant Difference (HSD) (p< 0.05). Results: There was a significant difference in the upregulation of sTRAIL and TNF-α level indicated by the increased annexin V, caspase-3, and caspase-8 expression binding between groups (p< 0.05). Conclusion: MSCs Secretome and CSF-2 could significantly increase the activity of PBMCs through the improvement of sTRAIL and TNF-α levels which could lead to the escalation of OS-SCs apoptosis through an enhanced expression of caspase 3, caspase 8 and annexin V binding in vitro

Phytochemical composition, biological activities and processing method of genus anisophyllea: a review

Anisophyllea disticha is one of the species of genus Anisophyllea that is not pharmacologically investigated despite its high utilization by the folklore. Leaf, stem and root of this plant are being used especially in treating diarrhea, dysentery, jaundice, refreshing men’s body, revitalizing the birth canal of women after delivering the baby as well as relieving weariness and body aches. The medicinal properties of A. disticha are predicted based on a literature review that provides information on secondary metabolites present and biological activities of this genus. The most reported biological activities comprise of antimicrobial, antioxidant, anti-diabetic and DNA damaging activity. From the previous studies, it can be observed that the extraction of bioactive components from this genus has been conducted using conventional method such as maceration, percolation and soxhlet. In this review, phytochemical contents, bioactivities and the methods of extraction of bioactive compounds from genus Anisophyllea have been broadly discussed. The advantages, as well as disadvantages of both conventional and non-conventional extraction methods have also been explained. Therefore, the extraction using non-conventional method like supercritical fluid extraction could provide the opportunity to obtain highly purified chemical constituents with potential in the application of various fields