Expression Characteristics of miR-10b in Nasopharyngeal Carcinoma

Aim: Nasopharyngeal carcinoma (NPC) is a highly malignant neoplasm characterized with aggressive local invasion and strong tendency of distant metastasis. MicroRNAs (miRNAs) are small noncoding RNAs that play a critical role in the pathogenesis of various malignant tumors. Our previous study aims to explore miRNAs′ expression characteristics in NPC tissue, which is barely unknown. Methods: A total of 45 NPC samples (26 metastatic and 19 nonmetastatic) and 16 chronic nasopharyngitis samples were analyzed. miR-10b level was determined by in situ hybridization using digoxigenin-labeled locked nucleic acid-based probe, and latent membrane protein-1 (LMP-1) expression were detected by immunohistochemistry. Results: We show that miR-10b is differentially expressed in NPC tissues. Furthermore, we correlate the expression level of miR-10b with Epstein-Barr virus encoding LMP-1. Overexpression of miR-10b is also found to relate with the clinical phases of NPC. Conclusion: These findings strongly suggest that miR-10b plays an important role in the NPC development

MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

Nasopharyngeal carcinoma (NPC) causes severe oncogenic lesions in the nasopharynx. CD47, a transmembrane integrin-associated protein, plays a key role in the ability of tumor cells to escape phagocytosis, working as an immune checkpoint in the immune response. Besides this role, CD47 has been reported to regulate cell proliferation and migration. The present study addresses the relationship between CD47 and microRNA-200a and examines their regulatory mechanisms in NPC. Bioinformatics analyses and dual-luciferase reporter assays were used to confirm the putative relationship between miR-200a and CD47, and their interaction was further detected using western blotting and RT-PCR. Further, results showed that miR-200a affect NPC cell proliferation, migration, and invasion by regulating CD47. A cell phagocytosis assay showed that miR-200a and a CD47 monoclonal antibody increased the sensitivity of NPC cells to macrophage phagocytosis by inhibiting the functions of CD47. Additionally, miR-200a expression was suppressed and CD47 expression increased in both clinical NPC tissues and cell lines. Taken together, these results show the miR-200a/CD47 combination as a potential therapeutic for treatment of NPC

Study on Breakage Mechanism in the Swirl Generating Stage of an Oil-Water Separator for Marine Oil Extraction and Its Verification

High-efficient oil-water separator is badly-needed in marine oil extraction. Droplet breakage is common during the conversion from pipe to swirl flow in separators. Avoiding oil droplets break into small ones in the swirl generating stage is beneficial to improve the separator's separation efficiency. Information regarding the breakage mechanism and dispersed droplet distribution is critical for optimum design of the conversion structure, such as guiding vanes and prediction of the oil-water separation performance. However, little work has been related to the study of droplet sizes in a swirl flow produced by guiding vanes. The present work focuses on the oil droplet sizes generated by the passage of oil-water mixture goes through guiding vanes in a vane-type separator and the different breakage mechanism. Experiments were performed under different flow rates and maximum droplet sizes were measured in situ downstream from the guiding vanes. The maximum droplet size was found to fit a modified-T model. Besides, Modified-T model was found to fit different studies data the best in the noncoalescence system. The studies shows that reducing the energy loss also reduces the probability of droplet breakage which put forward a new method to improve the separator's design

Ionic Exchange of Metal−Organic Frameworks for Constructing Unsaturated Copper Single‐Atom Catalysts for Boosting Oxygen Reduction Reaction

International audienc

Efficient industrial-current-density acetylene to polymer-grade ethylene via hydrogen-localization transfer over fluorine-modified copper

International audienceAbstract Electrocatalytic acetylene semi-hydrogenation to ethylene powered by renewable electricity represents a sustainable pathway, but the inadequate current density and single-pass yield greatly impedes the production efficiency and industrial application. Herein, we develop a F-modified Cu catalyst that shows an industrial partial current density up to 0.76 A cm −2 with an ethylene Faradic efficiency surpass 90%, and the maximum single-pass yield reaches a notable 78.5%. Furthermore, the Cu-F showcase the capability to directly convert acetylene into polymer-grade ethylene in a tandem flow cell, almost no acetylene residual in the production. Combined characterizations and calculations reveal that the Cu δ+ (near fluorine) enhances the water dissociation, and the generated active hydrogen are immediately transferred to Cu 0 (away from fluorine) and react with the locally adsorbed acetylene. Therefore, the hydrogen evolution reaction is surpassed and the overall acetylene semi-hydrogenation performance is boosted. Our findings provide new opportunity towards rational design of catalysts for large-scale electrosynthesis of ethylene and other important industrial raw