Pengaruh Ekstrak Kulit dan Jus Buah Delima Putih (Punica granatum L.) Terhadap Titer Antibodi Ayam Kampung Super yang Divaksin Newcastle Disease

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kulit dan buah delima terhadap titer antibodi ayam kampung super yang divaksin Newcastle Disease. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Sampel terdiri dari 40 ekor ayam kampung super yang dibagi acak menjadi 4 kelompok perlakuan. Perlakuan yang diberikan adalah K- tidak divaksin. K+, P1 dan P2 divaksin dengan ND Lasota. Kemudian setelah titer antibodi ayam rendah, K- diberikan aquadest steril, K+ diberikan CMC-Na 0,5 %, P1 diberikan ekstrak kulit delima (200 mg/kg BB), P2 diberikan jus buah delima (3 ml/kg BB). Sampel berupa serum di uji HI untuk mengetahui titer antibodi. Hasil rata-rata jumlah titer antibodi uji pertama pada K- yaitu (log 2) 1,10a±0,994, pada K+ yaitu (log 2) 4,60b±0,699, pada P1 yaitu (log 2) 5,90c±0,876, dan pada P2 yaitu (log 2) 7,20d±0,632. Dan pada uji kedua pada K- yaitu (log 2) 0,20a±0,422, pada K+ yaitu (log 2) 4,90b±0,876, pada P1 yaitu (log 2) 6,50c±0,707, dan pada P2 yaitu (log 2) 8,00d±0,816. Ada perbedaan yang nyata mengenai peningkatan titer antibodi pada masing-masing perlakuan. Berdasarkan hasil dapat disimpulkan bahwa ada pengaruh ekstrak kulit dan jus buah delima terhadap titer antibodi ayam kampung super yang divaksin Newcastle Disease. Penelitian disarankan untuk menggunakan jus buah delima karena lebih efektif

Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea Canephora)

This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group

Pola Pertumbuhan Ayam Broiler Strain Lohmann Berdasarkan Osteometri Tulang Sayap

Tujuan dari penelitian ini adalah untuk mengetahui pola pertumbuhan dan osteometri serta untuk mengetahui titik osteometri tulang sayap ayam broiler strain Lohmann. Pengukuran tulang sayap dilakukan dengan mengukur panjang, lebar, keliling dan barat tulang dengan menggunakan jangka sorong dengan ketelitian 0,05 mm. Hasil penelitian pola pertumbuhan ayam broiler strain Lohmann tulang sayap pada umur 7, 21 dan 35 hari, diperoleh hasil yang signifikan (p<0.05) terhadap nilai rata-rata panjang, lebar (proximal, corpus dan distal), keliling (proximal, corpus dan distal) dan berat pada os scapula, os coracoid dan os clavicula dan tulang-tulang alat penyusun gerak bebas yang terdiri dari os humerus, os radius, os ulna, os metacarpal I, os metacarpal II, os metacarpal III, ossa digiti I, ossa digiti II dexter phalang I dan Phalang II, ossa digiti II sinister Phalang I dan Phalang II serta os digiti III. Data di analisis menggunakan Analysis of Variance (ANOVA) selanjutnya diuji menggunakan uji Post Hoc Duncan. Ayam broiler strain Lohmann memiliki titik pertumbuhan maksimum saat berumur 21 hari yang ditunjukkan dengan terlihat ada titik optimal dan di lanjutkan dengan pertumbuhan yang melambat. Pada titik ini memicu pembentukan daging pada ayam broiler strain Lohmann sebelum ayam mencapai batas pertumbuhan

Toxic Effect of Robusta Coffee Extract (Coffea Canephora) on Bali Cattle Spermatozoa After Equilibration

The aim of this study was examine the effect of robusta coffee in Bali cattle semen. A total of six cattles cement were given four treatments each P0 without adding Robusta coffee extract, P1 was given an additional 5%, P2 10%, and P3 15%. The results are observed by assessing motility, viability, and abnormalities. The data obtained were analyzed by Analysis of Variance (ANOVA) and continued with the duncan. Based on the analysis of variance in obtaining the results of the study, it was shown motility of P0 was significantly different from P1, P2, and P3 (p<0.05). Viability of P0 (control) different significantly from P1 , P2, and P3 (p<0.05). P3 motility different significantly from P0, P1, and P2 (p<0.05). Addition of robusta coffee extract of 10% and 15% decreased spermatozoa quality

Pengaruh Ekstrak Kulit dan Jus Buah Delima Putih (Punica Granatum L.) terhadap Titer Antibodi Ayam Kampung Super yang Divaksin Newcastle Disease

Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kulit dan buah delima terhadap titer antibodi ayam kampung super yang divaksin Newcastle Disease. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Sampel terdiri dari 40 ekor ayam kampung super yang dibagi acak menjadi 4 kelompok perlakuan. Perlakuan yang diberikan adalah K- tidak divaksin. K+, P1 dan P2 divaksin dengan ND Lasota. Kemudian setelah titer antibodi ayam rendah, K- diberikan aquadest steril, K+ diberikan CMC-Na 0,5 %, P1 diberikan ekstrak kulit delima (200 mg/kg BB), P2 diberikan jus buah delima (3 ml/kg BB). Sampel berupa serum di uji HI untuk mengetahui titer antibodi. Hasil rata-rata jumlah titer antibodi uji pertama pada K- yaitu (log 2) 1,10a±0,994, pada K+ yaitu (log 2) 4,60b±0,699, pada P1 yaitu (log 2) 5,90c±0,876, dan pada P2 yaitu (log 2) 7,20d±0,632. Dan pada uji kedua pada K- yaitu (log 2) 0,20a±0,422, pada K+ yaitu (log 2) 4,90b±0,876, pada P1 yaitu (log 2) 6,50c±0,707, dan pada P2 yaitu (log 2) 8,00d±0,816. Ada perbedaan yang nyata mengenai peningkatan titer antibodi pada masing-masing perlakuan. Berdasarkan hasil dapat disimpulkan bahwa ada pengaruh ekstrak kulit dan jus buah delima terhadap titer antibodi ayam kampung super yang divaksin Newcastle Disease. Penelitian disarankan untuk menggunakan jus buah delima karena lebih efektif

Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination

Carcass Quality and Tetracycline Residues in Broiler Chicken Meat in Banyuwangi Traditional Market

This study aimed to examine the quality of broiler carcasses in the Banyuwangi traditional market with carcass quality examination based on SNI number 3924-2009 and tetracycline antibiotic residues test based on SNI number 7424:2008. A total of ten broiler chicken sellers as representative of almost all broiler chicken sellers in the Banyuwangi market, with each seller was taken for one carcass in this research. Carcass quality examination used a standart carcasses weight 1,0-1,3 kg. Carcass quality examination was carried out with descriptive analysis with reference to the physical characteristics of SNI (Standarisasi Nasional Indonesia). The bioassay method is used to test for tetracycline antibiotic residue. The result of the carcass quality examination showed that from the whole sample consisting of conformation, disjointed or broken bones and missing parts, discolorations, and cleanliness of broiler carcasses are good enough. The percentage quality of broiler carcasses quality I is 60%, quality II is 30% and quality III is 10%. The examination of antibiotic tetracycline residues in broiler meat showed negative results indicating that no feeds containing additional antibiotics made carcasses in the traditional Banyuwangi market safe for consumption

Deteksi Residu Antibiotik Oksitetrasiklin pada Susu Kambing Peranakan Etawah di Kelurahan Kalipuro, Banyuwangi dengan Uji Bioassay

Oksitetrasiklin diketahui sebagai salah satu antibiotik yang sering digunakan dalam menangani kasus mastitis pada hewan ternak. Penelitian ini merupakan penelitian deskriptif untuk mengetahui ada atau tidaknya residu oksitetrasiklin pada susu kambing Peranakan Etawah (PE) di Kelurahan Kalipuro, Banyuwangi. Sampel susu di koleksi kemudian dilakukan pengujian residu antibiotik dengan metode uji bioassay menggunakan bakteri standar Bacillus cereus ATCC 11778. Penelitian yang dilakukan pada 26 sampel susu yang di ambil secara acak berasal dari ternak kambing perah PE di dapatkan hasil yang negatif. Hal ini di tunjukkan dengan tidak terbentuknya zona hambat di sekitar well yang diisi sampel susu. Berbeda dengan kontrol postif oksitetrasiklin dengan konsentrasi 1 µg/ml yang menghasilkan zona hambatan sekitar 18 mm. Dapat disimpulkan bahwa 100% dari 26 jumlah sampel susu kambing PE yang di ambil secara acak dan di periksa dari Kelurahan Kalipuro, Banyuwangi tidak mengandung residu antibiotik oksitetrasiklin

The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi

Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score