Comparison of Gross Primary Productivity Derived from GIMMS NDVI3g, GIMMS, and MODIS in Southeast Asia

Gross primary production (GPP) plays an important role in the net ecosystem exchange of CO2 between the atmosphere and terrestrial ecosystems. It is particularly important to monitor GPP in Southeast Asia because of increasing rates of tropical forest degradation and deforestation in the region in recent decades. The newly available, improved, third generation Normalized Difference Vegetation Index (NDVI3g) from the Global Inventory Modelling and Mapping Studies (GIMMS) group provides a long temporal dataset, from July 1981 to December 2011, for terrestrial carbon cycle and climate response research. However, GIMMS NDVI3g-based GPP estimates are not yet available. We applied the GLOPEM-CEVSA model, which integrates an ecosystem process model and a production efficiency model, to estimate GPP in Southeast Asia based on three independent results of the fraction of photosynthetically active radiation absorbed by vegetation (FPAR) from GIMMS NDVI3g (GPPNDVI3g), GIMMS NDVI1g (GPPNDVI1g), and the Moderate Resolution Imaging Spectroradiometer (MODIS) MOD15A2 FPAR product (GPPMOD15). The GPP results were validated using ground data from eddy flux towers located in different forest biomes, and comparisons were made among the three GPPs as well as the MOD17A2 GPP products (GPPMOD17). Based on validation with flux tower derived GPP estimates the results show that GPPNDVI3g is more accurate than GPPNDVI1g and is comparable in accuracy with GPPMOD15. In addition, GPPNDVI3g and GPPMOD15 have good spatial-temporal consistency. Our results indicate that GIMMS NDVI3g is an effective dataset for regional GPP simulation in Southeast Asia, capable of accurately tracking the variation and trends in long-term terrestrial ecosystem GPP dynamics

The complete chloroplast genome sequences of an endangered orchid species Paphiopedilum wenshanense (Orchidaceae)

Paphiopedilum wenshanense, a species of the family Orchidaceae, is endangered in the world with highly ornamental and biological value, the morphology of P. wenshanense is very similar to its relative species, P. bellatulum and P. concolor. However, there are few studies on the molecular biology and phylogeny of this species currently. Therefore, we report its complete chloroplast (cp) genome sequence at the first time, hoping to provide a foundation for its future phylogenetic analysis. The complete chloroplast genome of P. wenshanense was 161,750 bp in size, which contained a large single-copy (LSC) region of 90,656 bp, a small single-copy (SSC) region of 1886 bp, and two inverted repeat (IR) regions of 34,604 bp each. The total GC content was 35.66%. The genome encodes 38 transfer RNA genes, eight ribosomal RNA genes, and 80 protein-coding genes. The phylogenetic analysis indicated that the genetic relationship between P. wenshanense and P. concolor was very close

The complete chloroplast genome sequence of Xylia xylocarpa

The first complete chloroplast genome (cpDNA) sequence of Xylia xylocarpa was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 161,288 bp in length, contains a large single copy region (LSC) of 89,186 bp and a small single copy region (SSC) of 19,354 bp, which were separated by a pair of inverted repeats (IR) regions of 26,370 bp. The genome contains 131 genes, including 86 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. The overall GC content of the whole genome is 36.6%, and the corresponding values of the LSC, SSC, and IR regions are 34.1%, 30.8%, and 42.8%, respectively. Further phylogenomic analysis showed that X. xylocarpa clustered in a unique clade in Caesalpinioideae subfamily

The complete chloroplast genome sequence of Fosbergia shweliensis, an endemic species to Yunnan of China

The first complete chloroplast genome (cpDNA) sequence of Fosbergia shweliensis was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 154,717 bp in length, contains a large single-copy region (LSC) of 84,747 bp and a small single-copy region (SSC) of 18,230 bp, which were separated by a pair of inverted repeats (IR) regions of 25,870 bp. The genome contains 130 genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 36 transfer RNA genes. Further phylogenomic analysis showed that F. shweliensis was close to Coffea genus in the family Rubiaceae

The complete chloroplast genome sequence of Taxus yunnanensis

The first complete chloroplast genome (cpDNA) sequence of Taxus yunnanensis was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 129,190 bp in length. Like other species of taxus genus, the chloroplast genome of T. yunnanensis has lost one of the large inverted repeats (IRs). The genome contains 116 genes, including 82 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. Further phylogenomic analysis showed that T. yunnanensis closed to T. brevifolia in Lauraceae family

Table_2_Evaluation and analysis of multidrug resistance- and hypervirulence-associated genes in carbapenem-resistant Pseudomonas aeruginosa strains among children in an area of China for five consecutive years.XLSX

IntroductionCarbapenem-resistant Pseudomonas aeruginosa (CRPA) is a growing threat. It is urgent to investigate the multidrug resistance and high virulence of CRPA to provide a basis for infection control and rational use of antibiotics.MethodsA retrospective study of 56 nonduplicated CRPA isolates was conducted.ResultsCRPA mainly came from the intensive care unit (ICU) and was mostly isolated from sputum samples. The carbapenem resistance rates of P. aeruginosa were 21.37% (2016), 10.62, 5.88, 10 and 13.87% from 2016 to 2020, respectively. Carbapenem-resistant enzymes and aminoglycoside-modifying enzyme-encoding genes were detected in all isolates, and extended-spectrum β-lactamase and cephalosporin enzyme-encoding genes were present in 96.43 and 80.38% of isolates, respectively. The detection rate of OprM showed a statistically significant difference (p DiscussionThe detection rates of various resistance and virulence genes were high, and the coexistence phenomenon was serious. In clinical practice, antibiotics should be used reasonably based on different drug resistance genes to ensure the rationality and safety of patient medication.</p

Whole-Genome Sequence Analysis of an Endophytic Fungus <i>Alternaria</i> sp. SPS-2 and Its Biosynthetic Potential of Bioactive Secondary Metabolites

As one of the commonly isolated endophytic fungi, Alternaria has been known for the production of numerous secondary metabolites (SMs). However, its detailed genomic features and SM biosynthetic potential have not been extensively studied thus far. The present work focuses on the whole-genome sequencing and assembly of an endophytic strain Alternaria sp. SPS-2 derived from Echrysantha chrysantha Lindl. and gene annotation using various bioinformatic tools. The results of this study suggested that the genome of strain SPS-2 was 33.4 Mb in size with a GC content of 51% and an N50 scaffold of 2.6 Mb, and 9789 protein-coding genes, including 644 CAZyme-encoding genes, were discovered in strain SPS-2 through KEGG enrichment analysis. The antiSMASH results indicated that strain SPS-2 harbored 22 SM biosynthetic gene clusters (BGCs), 14 of which are cryptic and unknown. LS–MS/MS and GNPS-based analyses suggested that this endophytic fungus is a potential producer of bioactive SMs and merits further exploration and development

Thoughts on the Present Situation of the Development of Rural Animal Husbandry and Its Relationship with the Construction of Ecological Agriculture and the Revitalization of Rural Industry

After several rounds of phased ups and downs of development, rural animal husbandry finally presents a shrinking and depressed development situation under the comprehensive action of the failure of transformation and upgrading and the increasing production costs and breeding risks. Actively exploring and guiding the healthy and sound development of rural animal husbandry is not only an important measure to promote the construction of ecological agriculture, but also the main starting point to realize the revitalization of rural industry. This paper analyzes the present situation of the development of rural animal husbandry in recent years, the influence of animal husbandry on the construction of ecological agriculture and the revitalization of rural industry, and puts forward the corresponding measures, in order　to promote the steady and efficient development of animal husbandry in the grand strategy of revitalizing rural industry

Table_1_Evaluation and analysis of multidrug resistance- and hypervirulence-associated genes in carbapenem-resistant Pseudomonas aeruginosa strains among children in an area of China for five consecutive years.docx

IntroductionCarbapenem-resistant Pseudomonas aeruginosa (CRPA) is a growing threat. It is urgent to investigate the multidrug resistance and high virulence of CRPA to provide a basis for infection control and rational use of antibiotics.MethodsA retrospective study of 56 nonduplicated CRPA isolates was conducted.ResultsCRPA mainly came from the intensive care unit (ICU) and was mostly isolated from sputum samples. The carbapenem resistance rates of P. aeruginosa were 21.37% (2016), 10.62, 5.88, 10 and 13.87% from 2016 to 2020, respectively. Carbapenem-resistant enzymes and aminoglycoside-modifying enzyme-encoding genes were detected in all isolates, and extended-spectrum β-lactamase and cephalosporin enzyme-encoding genes were present in 96.43 and 80.38% of isolates, respectively. The detection rate of OprM showed a statistically significant difference (p DiscussionThe detection rates of various resistance and virulence genes were high, and the coexistence phenomenon was serious. In clinical practice, antibiotics should be used reasonably based on different drug resistance genes to ensure the rationality and safety of patient medication.</p