Detergent-resistant plasma membrane proteome to elucidate microdomain functions in plant cells

Although proteins and lipids have been assumed to be distributed homogeneously in the plasma membrane (PM), recent studies suggest that the PM is in fact non-uniform structure that includes a number of lateral domains enriched in specific components (i.e., sterols, sphingolipids, and some kind of proteins). These domains are called as microdomains and considered to be the platform of biochemical reaction center for various physiological processes. Microdomain is able to be extracted as detergent-resistant membrane (DRM) fractions, and DRM fractions isolated from some plant species have been used for proteome and other biochemical characterizations to understand microdomain functions. Profiling of sterol-dependent proteins using a putative microdomain-disrupting agent suggests specific lipid–protein interactions in the microdomain. Furthermore, DRM proteomes dynamically respond to biotic and abiotic stresses in some plant species. Taken together, these results suggest that DRM proteomic studies provide us important information to understand physiological functions of microdomains that are critical to prosecute plant’s life cycle successfully in the aspect of development and stress responses

Development of a New Geomaterial for Base Isolation Foundations

Design method of rational composite foundation capable of reducing seismic response of structures was studied. Analysis of the effects of damping materials on the reduction of seismic response of a full-scale structure was performed. Shaking experiment and seismic observation for model foundations confirming the damping effects were conducted and development of new damping material are described

RELATIONSHIPS BETWEEN THE MAXIMUM SHOULDER EXTERNAL ROTATION ANGLE DURING THROWING AND PHYSICAL VARIABLES

To our knowledge, excessive shoulder external rotation may increase elbow valgus stress during throwing (2005). Inhibition of the greater shoulder external rotation angle could prevent elbow injury during throwing. Thus the purpose of this study was to determine physical factors that would increase the maximum shoulder external rotation (MER) angle