Intersubject and intrasubject variability of potential plasma and urine metabolite and protein biomarkers in healthy human volunteers

A limited understanding of intersubject and intrasubject variability hampers effective biomarker translation from in vitro/in vivo studies to clinical trials and clinical decision support. Specifically, variability of biomolecule concentration can play an important role in interpretation, power analysis, and sampling time designation. In the present study, a wide range of 749 plasma metabolites, 62 urine biogenic amines, and 1,263 plasma proteins were analyzed in 10 healthy male volunteers measured repeatedly during 12 hours under tightly controlled conditions. Three variability components in relative concentration data are determined using linear mixed models: between (intersubject), time (intrasubject), and noise (intrasubject). Biomolecules such as 3-carboxy-4-methyl-5-propyl-2-furanpropanoate, platelet-derived growth factor C, and cathepsin D with low noise potentially detect changing conditions within a person. If also the between component is low, biomolecules can easier differentiate conditions between persons, for example cathepsin D, CD27 antigen, and prolylglycine. Variability over time does not necessarily inhibit translatability, but requires choosing sampling times carefully.Analytical BioScience

Large‐scale ducting of Pc1 pulsations observed by Swarm satellites and multiple ground networks

Abstract Low Earth orbit satellites frequently encounter Pc1 pulsations, but most have been observed with limited latitudinal extent or short lifetime. In this study we analyze two large‐scale Pc1 pulsations (both latitudinally wide and long‐lasting) generated by ionospheric ducting effect using Swarm and ground magnetometers on 25 June and 3 September 2015. Swarm observed the 25 June pulsations on both dayside and nightside during the storm time substorm (a strong geomagnetic storm on 23 June with $D_{st} = − 204~nT$). We found the Pc1 pulsations were pervasive in both magnetic local time sectors of dayside and nightside for at least 2 hr. Another large Pc1 pulsation on 3 September was observed during a nonstorm substorm period. We conclude that (1) ionospheric ducting can transmit Pc1 waves to a wide range of L shells, (2) geomagnetic storm is not a prerequisite for such large‐scale ducting, and (3) wave intensity can abruptly decrease across sharp gradients in the ionospheric plasma density