Ablation of TSC2 Enhances Insulin Secretion by Increasing the Number of Mitochondria through Activation of mTORC1

) mice. The present study examines the effects of TSC2 ablation on insulin secretion from pancreatic beta cells. mice and TSC2 knockdown insulin 1 (INS-1) insulinoma cells treated with small interfering ribonucleic acid were used to investigate insulin secretion, ATP content and the expression of mitochondrial genes. mice exhibit hyperinsulinemia due to an increase in the number of mitochondria as well as enlargement of individual beta cells via activation of mTORC1.Activation of mTORC1 by TSC2 ablation increases mitochondrial biogenesis and enhances insulin secretion from pancreatic beta cells

膵β細胞におけるRac1の恒常的活性化はF-actinの脱重合を促進させるが、膵β細胞量増加やインスリン分泌促進には影響しない

博士（医学）神戸大

HER3 mRNA and protein expression levels in breast and ovarian cancer.

(A) HER3 expression in breast cancer in TMA (EdgeSeq and IHC H-score), TCGA (RNA-seq and RPPA) and CCLE (RNA-seq and RPPA) data sets and in (B) ovarian cancer TMA (EdgeSeq and IHC H-score) and CCLE (RNA-seq and RPPA) data sets. The rightmost graph in each panel displays the mRNA:protein correlation plot for each respective cancer type and data set. For the box-and-whisker plot, the line in the box represents the median and whiskers indicate 5th and 95th percentiles.</p

TMA EdgeSeq and TCGA gene expression score averages by cancer type and gene signatures for immune cell, housekeeping, cell cycle, and cancer-associated fibroblast genes.

(XLSX)</p

HER2 and HER3 mRNA and protein expression.

(A) Correlation of mRNA and protein expression of HER2 and HER3 in TMA (via EdgeSeq and immunohistochemistry [IHC] H-score) and CCLE (via RNA-seq and RPPA) data sets. Correlation coefficients were calculated with R > 0 and approaching one signifying a positive correlation. No data were available for HER2-cancers using visual examination. (B) HER3 mRNA (EdgeSeq) and protein (IHC H-score) expression from TMA data set. (C) TMA cancer types/subtypes sorted by HER3 IHC positivity (IHC 3+ and 2+). IHC scores of 3+ and 2+ were considered signal intensity that was positive for HER3 expression. For the box-and-whisker plot, the line in the box represents the median and whiskers indicate 5th and 95th percentiles. BC, breast cancer.</p