10 research outputs found
Synthesis and optical reactivity of 6,13-α-diketoprecursors of 2,3,9,10-tetraalkylpentacenes in solution, films and crystals
Tetraalkylpentacenes having alkyl chains at 2,3,9,10-positions (Et-PEN, Pr-PEN and Hex-PEN) were prepared from their precursors Et-PDK, Pr-PDK and Hex-PDK, respectively. Photoreactions proceeded both in solutions, thin-films, and crystals, thus the properties of Et-PDK in films can be studied despite the instability of the pentacenes in solution. Et-PEN showed significantly different aggregation-nature compared with the parent pentacene. The hole mobilities of Et-PEN and Pr-PEN in films were 3.4 [times] 10-6 and 8.1 [times] 10-7 cm2 V-1 s-1, respectively, determined by space-charge-limited current measurement, comparable with the order 10-6 cm2 V-1 s-1 of the electron mobility of Alq3
Evaluation of semiconducting molecular thin films solution-processed via the photoprecursor approach: the case of hexyl-substituted thienoanthracenes
Organic electronic devices are expected to be easily scalable and highly cost-effective, presuming the good solution processability of high-performance organic semiconductors. However, there are cases where an organic compound with promising semiconducting properties lacks adequate processability and does not form well-performing thin films through conventional solution-based deposition techniques. The photoprecursor approach, in which a soluble photoprecursor is solution-deposited on a substrate and then converted to a target material by in situ photoreaction, can be an effective means to evade such a problem. Herein, we describe a comparative evaluation of thin films deposited by three different methods; i.e., vacuum deposition, photoprecursor approach, and direct spin coating. Two highly crystalline molecular semiconductors, hexyl-substituted anthra[1,2-b:4,3-b':5,6-b'':8,7-b''']tetrathiophene (C6-ATT) and anthra[1,2-b:5,6-b']dithiophene (or bent anthradithiophene, C6-BADT), are employed in this study along with the corresponding newly synthesized α-diketone-type photoprecursors. In the case of C6-ATT, thin films prepared through the photoprecursor approach are as good as those obtained by vacuum deposition in terms of surface smoothness and space-charge-limited-current (SCLC) mobility, while direct spin coating affords highly inhomogeneous films. For C6-BADT, on the other hand, employment of the photoprecursor approach is not as effective, albeit it is still advantageous as compared to direct spin coating. These results highlight the power and limitations of the photoprecursor approach, and will serve as a basis for the preparation of practically useful organic devices through this unique approach
Possible involvement of zinc transporter ZIP13 in myogenic differentiation
Abstract Ehlers–Danlos syndrome spondylodysplastic type 3 (EDSSPD3, OMIM 612350) is an inherited recessive connective tissue disorder that is caused by loss of function of SLC39A13/ZIP13, a zinc transporter belonging to the Slc39a/ZIP family. We previously reported that patients with EDSSPD3 harboring a homozygous loss of function mutation (c.221G > A, p.G64D) in ZIP13 exon 2 (ZIP13 G64D ) suffer from impaired development of bone and connective tissues, and muscular hypotonia. However, whether ZIP13 participates in the early differentiation of these cell types remains unclear. In the present study, we investigated the role of ZIP13 in myogenic differentiation using a murine myoblast cell line (C2C12) as well as patient-derived induced pluripotent stem cells (iPSCs). We found that ZIP13 gene expression was upregulated by myogenic stimulation in C2C12 cells, and its knockdown disrupted myotubular differentiation. Myocytes differentiated from iPSCs derived from patients with EDSSPD3 (EDSSPD3-iPSCs) also exhibited incomplete myogenic differentiation. Such phenotypic abnormalities of EDSSPD3-iPSC-derived myocytes were corrected by genomic editing of the pathogenic ZIP13 G64D mutation. Collectively, our findings suggest the possible involvement of ZIP13 in myogenic differentiation, and that EDSSPD3-iPSCs established herein may be a promising tool to study the molecular basis underlying the clinical features caused by loss of ZIP13 function
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Research data for paper Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS
Research data for paper Molecular and functional characterization of an evolutionarily conserved CREB-binding protein in the Lymnaea CNS (FASEB Journal October 2022).
This dataset consists of two Excel spreadsheets containing molecular biological and electrophysiological data, respectively.
The data used for Figure 2 was obtained by measuring the expression levels of the CBP mRNA in different tissues of Lymnaea by using qRT-PCR. The data used for the graph and associated statistical analysis show that the expression level is highest in the central nervous system (CNS).
The data used for Figure 6F was obtained by intracellular microelectrophysiology to measure the amplitude of the monosynaptic EPSP evoked in the motoneuron B1 by the electrical stimulation of the Cerebral Giant Cell (CGC) under different experimental conditions. The data used for the graph and associated statistical analysis show that injection of cAMP into the CGC cell body increased EPSP amplitude in the B1 motoneuron, but pre-treatment with either of the three HAT inhibitors prevented the cAMP-induced increase of EPSP. It also shows that 5'AMP that was used as a control for cAMP did not increase the amplitude of the B1 EPSP.</p