Modeling and Validation of a Data Process Unit Control for Space Applications

International audienceData process unit (DPU) is a typical embedded system. It is widely used in space applications to collect data from sensors, process data and send the data to its upper master computer. In this paper, we use the BIP framework to model and validate a DPU system of a real space application. We first build the system model including the control software, hardware and the environment. Validation is by extensive simulation of a monitored system obtained as the composition of the DPU model with monitors. A monitor checks a requirement by continuously sensing the state of the model and reaching an error state if the requirement is violated. We checked fault-tolerance for di erent fault models and detected several errors that under some conditions, could correspond to real implementation errors

Visibility and distortion measurement for no-reference dehazed image quality assessment via complex contourlet transform

Recently, most dehazed image quality assessment (DQA) methods mainly focus on the estimation of remaining haze, omitting the impact of distortions from the side effect of dehazing algorithms, which lead to their limited performance. Addressing this problem, we proposed a learning both Visibility and Distortion Aware features no-reference (NR) Dehazed image Quality Assessment method (VDA-DQA). Visibility aware features are exploited to characterize clarity optimization after dehazing, including the brightness, contrast, and sharpness aware feature extracted by complex contourlet transform (CCT). Then, distortion aware features are employed to measure the distortion artifacts of images, including the normalized histogram of local binary pattern (LBP) from the reconstructed dehazed image and the statistics of the CCT sub-bands corresponding to chroma and saturation map. Finally, all the above features are mapped into the quality scores by the support vector regression (SVR). Extensive experimental results on six public DQA datasets verify the superiority of proposed VDA-DQA in terms of the consistency with subjective visual perception, and outperforms the state-of-the-art methods.The source code of VDA-DQA is available at https://github.com/li181119/VDA-DQA

Identification and characterization of a 25-lncRNA prognostic signature for early recurrence in hepatocellular carcinoma

Background Early recurrence is the major cause of poor prognosis in hepatocellular carcinoma (HCC). Long non-coding RNAs (lncRNAs) are deeply involved in HCC prognosis. In this study, we aimed to establish a prognostic lncRNA signature for HCC early recurrence. Methods The lncRNA expression profile and corresponding clinical data were retrieved from total 299 HCC patients in TCGA database. LncRNA candidates correlated to early recurrence were selected by differentially expressed gene (DEG), univariate Cox regression and least absolute shrinkage and selection operator (LASSO) regression analyses. A 25-lncRNA prognostic signature was constructed according to receiver operating characteristic curve (ROC). Kaplan-Meier and multivariate Cox regression analyses were used to evaluate the performance of this signature. ROC and nomogram were used to evaluate the integrated models based on this signature with other independent clinical risk factors. Gene set enrichment analysis (GSEA) was used to reveal enriched gene sets in the high-risk group. Tumor infiltrating lymphocytes (TILs) levels were analyzed with single sample Gene Set Enrichment Analysis (ssGSEA). Immune therapy response prediction was performed with TIDE and SubMap. Chemotherapeutic response prediction was conducted by using Genomics of Drug Sensitivity in Cancer (GDSC) pharmacogenomics database. Results Compared to low-risk group, patients in high-risk group showed reduced disease-free survival (DFS) in the training (p < 0.0001) and validation cohort (p = 0.0132). The 25-lncRNA signature, AFP, TNM and vascular invasion could serve as independent risk factors for HCC early recurrence. Among them, the 25-lncRNA signature had the best predictive performance, and combination of those four risk factors further improves the prognostic potential. Moreover, GSEA showed significant enrichment of “E2F TARGETS”, “G2M CHECKPOINT”, “MYC TARGETS V1” and “DNA REPAIR” pathways in the high-risk group. In addition, increased TILs were observed in the low-risk group compared to the high-risk group. The 25-lncRNA signature negatively associates with the levels of some types of antitumor immune cells. Immunotherapies and chemotherapies prediction revealed differential responses to PD-1 inhibitor and several chemotherapeutic drugs in the low- and high-risk group. Conclusions Our study proposed a 25-lncRNA prognostic signature for predicting HCC early recurrence, which may guide postoperative treatment and recurrence surveillance in HCC patients

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.

G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ∼20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

The use of deep learning methods in low-dose computed tomography image reconstruction : a systematic review

Conventional reconstruction techniques, such as filtered back projection (FBP) and iterative reconstruction (IR), which have been utilised widely in the image reconstruction process of computed tomography (CT) are not suitable in the case of low-dose CT applications, because of the unsatisfying quality of the reconstructed image and inefficient reconstruction time. Therefore, as the demand for CT radiation dose reduction continues to increase, the use of artificial intelligence (AI) in image reconstruction has become a trend that attracts more and more attention. This systematic review examined various deep learning methods to determine their characteristics, availability, intended use and expected outputs concerning low-dose CT image reconstruction. Utilising the methodology of Kitchenham and Charter, we performed a systematic search of the literature from 2016 to 2021 in Springer, Science Direct, arXiv, PubMed, ACM, IEEE, and Scopus. This review showed that algorithms using deep learning technology are superior to traditional IR methods in noise suppression, artifact reduction and structure preservation, in terms of improving the image quality of low-dose reconstructed images. In conclusion, we provided an overview of the use of deep learning approaches in low-dose CT image reconstruction together with their benefits, limitations, and opportunities for improvement

Factors Influencing the Spatiotemporal Variability in the Irrigation Requirements of Winter Wheat in the North China Plain under Climate Change

The North China Plain is a major grain-producing area, but faces water scarcity, which directly threatens food security. The problem is more severe under climate change and the seasonal impact of climate change on winter wheat is different. Thus, it is of great importance to explore the spatiotemporal characteristics of irrigation requirements (IR) and the factors influencing IR in different growth periods of winter wheat, but it has not received much attention. Therefore, we used relative contribution, partial correlation and path analyses to assess the spatiotemporal characteristics of the IR and primary factors influencing the IR of winter wheat in various growing stages in the North China Plain. The results indicated that wind speed and net solar radiation showed a significant downward trend; no prominent trend was noted in IR (multiyear average, 302.3 mm). Throughout the growing season of winter wheat, IR increased gradually from the southern to northern extent of the North China Plain. The irrigation demand of winter wheat in stage P2 (green-up to heading) was the largest. Furthermore, the dominant drivers of IR in terms of spatial distribution and inter-annual variation were phenological period (Phe), effective precipitation (Pe) and relative humidity (RH); however, the degree of their effects varied across the growth stages and growing regions of winter wheat. Each factor exerted both direct and indirect effects on IR and Phe exhibited the strongest indirect effect on IR. The major factors contributing most to IR were Pe and RH in the P1 stage (sowing to green-up) and Phe, Pe and RH in the P2 and P3 (heading to maturity) stages. Pe and RH limited IR, whereas Phe promoted it. Our findings will help improve agricultural water management in the future