Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Proceedings of the 29th EG-ICE International Workshop on Intelligent Computing in Engineering

This publication is the Proceedings of the 29th EG-ICE International Workshop on Intelligent Computing in Engineering from July 6-8, 2022. The EG-ICE International Workshop on Intelligent Computing in Engineering brings together international experts working on the interface between advanced computing and modern engineering challenges. Many engineering tasks require open-world resolution of challenges such as supporting multi-actor collaboration, coping with approximate models, providing effective engineer-computer interaction, search in multi-dimensional solution spaces, accommodating uncertainty, including specialist domain knowledge, performing sensor-data interpretation and dealing with incomplete knowledge. While results from computer science provide much initial support for resolution, adaptation is unavoidable and most importantly, feedback from addressing engineering challenges drives fundamental computer-science research. Competence and knowledge transfer goes both ways. &nbsp

Proceedings of the 29th EG-ICE International Workshop on Intelligent Computing in Engineering

This publication is the Proceedings of the 29th EG-ICE International Workshop on Intelligent Computing in Engineering from July 6-8, 2022. The EG-ICE International Workshop on Intelligent Computing in Engineering brings together international experts working on the interface between advanced computing and modern engineering challenges. Many engineering tasks require open-world resolution of challenges such as supporting multi-actor collaboration, coping with approximate models, providing effective engineer-computer interaction, search in multi-dimensional solution spaces, accommodating uncertainty, including specialist domain knowledge, performing sensor-data interpretation and dealing with incomplete knowledge. While results from computer science provide much initial support for resolution, adaptation is unavoidable and most importantly, feedback from addressing engineering challenges drives fundamental computer-science research. Competence and knowledge transfer goes both ways. &nbsp

Clinical analysis of metagenome next-generation sequencing for diagnosing invasive fungal disease in patients with early stage of hematopoietic stem cell transplantation

Objective To analyze the clinical outcomes of early invasive fungal disease (IFD) in patients after allogenetic hematopoietic stem cell transplantation (allo-HCST) with metagenomic next-generation sequencing (mNGS). Methods A retrospective analysis was conducted on patients undergoing allo-HCST in our Bone Marrow Transplantation Center between July 2021 and October 2022.These patients experienced one of the following conditions within 100 d after transplantation: ① Patients with persistent fever and negative blood culture after empiric antimicrobial therapy for 72 h or longer; ② Hyperpyrexia of unknown origin occurred again after effective anti-infection in the past; ③ Symptoms in lower respiratory tract associated with lung lesions on CT scan, and empiric anti-infective therapy was ineffective.Peripheral blood or bronchoscopic alveolar lavage fluid were tested with mNGS, and overall survival (OS) and non-relapse mortality (NRM) were analyzed. Results There were 60 patients enrolled in this study.For the peripheral blood samples of 47 cases and bronchoalveolar lavage fluid samples of 13 cases, mNGS found that 19 cases were negative to pathogens, 30 cases were non-fungal positive, and 11 case were fungal positive, including 3 cases of aspergillus, 5 cases of mucor, 2 cases of Candida tropicalis, and 1 case of Trichosporon asahii.Of the 11 patients with fungal positive, 8 achieved complete remission after antifungal therapy according to the mNGS results.The 1-year OS and NRM of the 60 patients were 70.0%(95%CI: 64.1%~75.9%) and 20.0%(95%CI: 11.9%~32.5%), respectively, while those of the fungal infection patients were 54.5%(95%CI: 49.5%~69.5%) and 36.4%(95%CI: 15.5%~70.3%), respectively.No significant differences were seen in 1-year OS (P=0.487) and 1-year NRM (P=0.358) among the negative, fungal infection and non-fungal infection patients, neither OS (P=0.238) and NRM (P=0.154) between the fungal infection and the non-fungal infection patients. Conclusion mNGS can rapidly diagnose the early IFD after allo-HSCT, which is helpful for timely and effective treatment and improves the prognosis of patients

Examining the Impact of China’s Poverty Alleviation on Nighttime Lighting in 831 State-Level Impoverished Counties

China’s poverty alleviation projects have made significant contributions to global poverty eradication. This study investigates the impact of China’s poverty alleviation projects on nighttime lighting in 831 state-level impoverished counties using the “NPP-VIIRS-like” dataset and discusses the difference of land use change under different nighttime light clusters in order to provide reference for future policy formulation and implementation. Our results show that the growth of total intensity of nighttime lighting (GRTNL) and the year-on-year growth rate of total intensity of nighttime lighting (YGRTNL) in China’s impoverished counties are 103.74% and 9.69% from 2013 to 2021, respectively, which are both higher than the average levels of all counties (67.16%, 6.77%) and non-poor counties (64.68%, 6.56%) in China during the same period. Additionally, we discovered that impoverished counties that lifted out of poverty earlier had significantly higher nighttime lighting intensity than those later. Regional analysis reveals that the growth of nighttime lighting intensity shows a trend of decreasing from the central (1550.89 nW·cm−2·sr−1) to the eastern (924.57), western (762.57), and northeastern regions (588.07), while the growth rate decreases from western regions (282.46%) to the eastern (189.13%), central (178.56%), and northeastern (108.07%). We also identified that Gini coefficient of nighttime lighting has a trend of “slow and short-term rise-rapid and continuous decline”. Moreover, nighttime lighting growth had similar trends with land use change, especially construction land. Overall, our study provides novel insights into the relationship between poverty alleviation effects and nighttime lighting in China’s impoverished counties, which could inform future policy-making and research in this area

Socializing with Smoker and Social Smoking Behavior among Chinese Male Smokers with Low Nicotine Dependence: The Mediating Roles of Belief of Smoking Rationalization and Smoker Identity

Background: Previous studies have shown that socializing with other smokers is an essential trigger for social smoking among smokers with a low nicotine dependence. This study further explored the mediating effects of the belief of smoking rationalization and smoker identity on the relationship between socializing with smokers and social smoking behavior. Methods: A cross-sectional design was conducted. A total of 696 low-nicotine-dependent smokers in China completed questionnaires that assessed socializing with smokers, social smoking behavior, smoker identity, and the belief of smoking rationalization. The mediating roles of the belief of smoking rationalization and smoker identity on the relationship between socializing with smokers and social smoking behavior were assessed by using SPSS 23 and AMOS 23. Results: The belief of smoking rationalization, smoker identity, socializing with smokers, and social smoking behavior were significantly and positively correlated with each other. In addition, this study found an independently mediated role for smoker identity in the relationship with smoker socialization and social smoking behavior, and a sequentially mediated role for smoking rationalization and smoker identity in this relationship. Conclusion: Reducing the belief of smoking rationalization and smoker identity may be conducive to reducing social smoking behavior for low-nicotine-dependent smokers when socializing with other smokers

Direct Oxygen-Oxygen Cleavage through Optimizing Interatomic Distances in Dual Single-atom Electrocatalysts for Efficient Oxygen Reduction Reaction

The oxygen reduction reaction (ORR) on transition single-atom catalysts (SACs) is sustainable in energy-conversion devices. However, the atomically controllable fabrication of single-atom sites and the sluggish kinetics of ORR have remained challenging. Here, we accelerate the kinetics of acid ORR through a direct O−O cleavage pathway through using a bi-functional ligand-assisted strategy to pre-control the distance of hetero-metal atoms. Concretely, the as-synthesized Fe−Zn diatomic pairs on carbon substrates exhibited an outstanding ORR performance with the ultrahigh half-wave potential of 0.86 V vs. RHE in acid electrolyte. Experimental evidence and density functional theory calculations confirmed that the Fe−Zn diatomic pairs with a specific distance range of around 3 Å, which is the key to their ultrahigh activity, average the interaction between hetero-diatomic active sites and oxygen molecules. This work offers new insight into atomically controllable SACs synthesis and addresses the limitations of the ORR dissociative mechanism