Effects of Black Pepper (Piper nigrum L.)

The chemical composition and antimicrobial mechanism of action of black pepper chloroform extract (BPCE) were investigated, as well as the potential antibacterial activities of BPCE against Escherichia coli and Staphylococcus aureus. The results showed that 1H-Cycloprop[e]azulen-7-ol, decahydro-1,1,7-trimethyl-4-methylene-, [1ar-(1aα,4aα,7β,7a,β,7bα.)]- (8.39%) and 2-methylene-4,8,8-trimethyl-4-vinyl-bicyclo[5.2.0]nonane (6.92%) were identified as the two primary components of BPCE. The release of intracellular transaminases from bacteria after being incubated with BPCE revealed that the bacterial cell walls and membranes were degraded and that protein synthesis was inhibited to some extent. The inhibition of bacterial Na+/K+-ATPase activity upon the addition of BPCE also indicated an enhanced permeability of bacterial cell membranes. Moreover, an analysis of hexokinase and pyruvate kinase activities showed that BPCE affected the metabolic rate of glycolysis and disrupted the normal metabolism of bacteria. This phenomenon was supported by an observed accumulation of lactic acid (LA) in the treated bacterial cells. Overall, our results indicated that BPCE damaged bacterial cell walls and membranes, which was followed by a disruption of bacterial cell respiration

Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair

Characterization of hMSCs and hMSC-chondrogenic pellets. (a) Flow cytometric analysis of the hematopoietic markers (CD34, CD45) and MSC markers (CD73, CD105) in hMSCs. The proportion of CD73-expressing cells was 99.7 ± 0.63 %; The proportion of CD105-expressing cells was 98.7 ± 1.21 %. Values represent means ± SEM; n = 3. (b) Three-dimensional pellet culture of the hMSC-chondrogenic pellet. (c) Alcian blue staining and (d) toluidine blue staining of glycosaminoglycans and proteoglycans revealed the chondrocyte-type appearance in the hMSC-chondrogenic pellets. Scale bar = 100 μm. (e,f) Immunohistochemistry for type II and type X collagen. Scale bar = 100 μm. (TIF 8130 kb

Fly-by galaxy encounters with multiple black holes produce star-forming linear wakes

We look for simulated star-forming linear wakes such as the one recently discovered by van Dokkum et al. (2023) in the cosmological hydrodynamical simulation ASTRID. Amongst the runaway black holes in ASTRID, none are able to produce clear star-forming wakes. Meanwhile, fly-by encounters, typically involving a compact galaxy (with a central black hole) and a star-forming galaxy (with a duo of black holes) reproduce remarkably well many of the key properties (its length and linearity; recent star formation, etc.) of the observed star-forming linear feature. We predict the feature to persist for approximately 100 Myr in such a system and hence constitute a rare event. The feature contains a partly stripped galaxy (with $M_{\rm gal}=10^9 \sim 10^{10}M_\odot$) and a dual BH system ($M_{\rm BH}=10^5 \sim 10^7\,M_\odot$) in its brightest knot. X-ray emission from AGN in the knot should be detectable in such systems. After $100\sim 200\,{\rm Myrs}$ from the first fly-by, the galaxies merge leaving behind a triple black hole system in a (still) actively star-forming early-type remnant of mass $\sim 5\times 10^{10}\,M_\odot$. Follow-up JWST observations may be key for revealing the nature of these linear features by potentially detecting the older stellar populations constituting the bright knot. Confirmation of such detections may therefore help discriminate a fly-by encounter from a massive BH wake to reveal the origin of such features.Comment: 8 pages, 5 figures, comments welcom

Triple and Quadruple Black Holes in the ASTRID Simulation at z∼2z \sim 2

We use the ASTRID cosmological hydrodynamic simulation to investigate the properties and evolution of triple and quadruple Massive Black Hole (MBH) systems at $z = 2-3$. Only a handful of MBH tuple systems have been detected to date. In ASTRID, we find $4\%$ of the $M_{\rm BH}>10^7\,M_\odot$ are in tuples with $\Delta r_{\rm max} < 200\,{\rm kpc}$. The tuple systems span a range of separations with the majority of the observable AGN systems at $\Delta r \sim 50-100$ kpc. They include some of the most massive BHs (up to $10^{10} \,M_\odot$) but with at least one of the components of $M_{\rm BH} \sim 10^7 \,M_\odot$. Tuples' host galaxies are typically massive with $M_* \sim 10^{10-11} \,M_\odot$. We find that $>10\%$ massive halos with $M_{\rm halo} > 10^{13} M_\odot$ host MBH tuples. Following the subsequent interactions between MBHs in tuples, we found that in $\sim 5\%$ of the triplets all three MBHs merge within a Gyr, and $15\%$ go through one merger. As a by-product of the complex multi-galaxy interaction of these systems, we also find that up to $\sim 5\%$ of tuples lead to runaway MBHs. In ASTRID, virtually all of the ultramassive black holes ($>10^{10} \,M_\odot$) have undergone a triple quasar phase while for BHs with $M_{\rm BH} \sim 10^9 \,M_\odot$ this fraction drops to $50\%$.Comment: 10 pages, 9 figures; comments welcom

Orbital and Radiative Properties of Wandering Intermediate-Mass Black Holes in the ASTRID Simulation

Intermediate-Mass Black Holes (IMBHs) of $10^3-10^6 \, M_\odot$ are commonly found at the center of dwarf galaxies. Simulations and observations convincingly show that a sizable population of IMBHs could wander off-center in galaxies. We use the cosmological simulation ASTRID to study the orbital and radiative properties of wandering IMBHs in massive galaxies at $z\sim3$. We find that this population of black holes has large orbital inclinations ($60^\circ\pm22^\circ$) with respect to the principal plane of the host. The eccentricity of their orbits is also significant ($0.6\pm0.2$) and decreases with time. Wandering IMBHs undergo spikes of accretion activity around the pericenter of their orbits, with rates $10^{-3}-10^{-5}$ times the Eddington rate and a median accretion duty cycle of $\sim 12\%$. Their typical spectral energy distribution peaks in the infrared at $\sim 11 \, \mu \rm m$ rest-frame. Assuming a standard value of $10\%$ for the matter-to-energy radiative efficiency, IMBHs reach $2-10$ keV X-ray luminosities $>10^{37} \, \mathrm{erg\,s^{-1}}$ for $\sim10\%$ of the time. This luminosity corresponds to fluxes $>10^{-15} \, \mathrm{erg \, s^{-1} \, cm^{-2}}$ within $10$ Mpc. They could be challenging to detect because of competing emissions from X-ray binaries and the interstellar medium. X-ray luminosities $> 10^{41} \, \mathrm{erg \, s^{-1}}$, in the hyper-luminous X-ray sources (HLXs) regime, are reached by $\sim 7\%$ of the IMBHs. These findings suggest that HLXs are a small subset of the wandering IMBH population, which is characterized by luminosities $10^3-10^4$ times fainter. Dedicated surveys are needed to assess the demographics of this missing population of black holes.Comment: Accepted for publication in MNRAS. This is the final version of the manuscript. 9 pages, 7 figure

Bacterium-Enabled Transient Gene Activation by Artificial Transcription Factor for Resolving Gene Regulation in Maize

Cellular functions are diversified through intricate transcription regulations, and an understanding gene regulation networks is essential to elucidating many developmental processes and environmental responses. Here, we employed the Transcriptional-Activator Like effectors (TALes), which represent a family of transcription factors that are synthesized by members of the γ-proteobacterium genus Xanthomonas and secreted to host cells for activation of targeted host genes. Through delivery by the maize pathogen, Xanthomonas vasicola pv. vasculorum, designer TALes (dTALes), which are synthetic TALes, were used to induce the expression of the maize gene glossy3 (gl3), a MYB transcription factor gene involved in the cuticular wax biosynthesis. RNA-Seq analysis of leaf samples identified 146 gl3 downstream genes. Eight of the nine known genes known to be involved in the cuticular wax biosynthesis were up-regulated by at least one dTALe. A top-down Gaussian graphical model predicted that 68 gl3 downstream genes were directly regulated by GL3. A chemically induced mutant of the gene Zm00001d017418 from the gl3 downstream gene, encoding aldehyde dehydrogenase, exhibited a typical glossy leaf phenotype and reduced epicuticular waxes. The bacterial protein delivery of artificial transcription factors, dTALes, proved to be a straightforward and powerful approach for the revelation of gene regulation in plants

A vast population of wandering and merging IMBHs at cosmic noon

Massive black holes in the centers of galaxies today must have grown by several orders of magnitude from seed black holes formed at early times. Detecting a population of intermediate mass black holes (IMBHs) can provide constraints on these elusive BH seeds. Here we use the large volume, cosmological hydrodynamical simulation Astrid, which includes IMBH seeds and dynamical friction to investigate the population of IMBH seeds. Dynamical friction is largely inefficient at sinking and merging seed IMBHs at high-z. This leads to an extensive population (several hundred per galaxy) of wandering IMBHs in large halos at z~2. A small fraction of these IMBHs are detectable as HLXs, Hyper Luminous X-ray sources. Importantly, at z ~ 2, IMBHs mergers produce the peak of GW events. We find close to a million GW events in Astrid between z=2-3 involving seed IMBH mergers. These GW events (almost all detectable by LISA) at cosmic noon should provide strong constraints on IMBH seed models and their formation mechanisms. At the center of massive galaxies, where the number of IMBHs can be as high as 10-100, SMBH-IMBH pairs can form. These Intermediate mass ratio inspirals (IMRIs) and extreme mass ratio inspirals (EMRIs), will require the next generation of milli-muHz space-based GW interferometers to be detected. Large populations of IMBHs around massive black holes will probe their environments and MBH causal structure

Molecular imaging research in atherosclerosis: A 23-year scientometric and visual analysis

Background: Cardiovascular and cerebrovascular diseases are major global health problems, and the main cause is atherosclerosis. Recently, molecular imaging has been widely employed in the diagnosis and therapeutic applications of a variety of diseases, including atherosclerosis. Substantive facts have announced that molecular imaging has broad prospects in the early diagnosis and targeted treatment of atherosclerosis.Objective: We conducted a scientometric analysis of the scientific publications over the past 23 years on molecular imaging research in atherosclerosis, so as to identify the key progress, hotspots, and emerging trends.Methods: Original research and reviews regarding molecular imaging in atherosclerosis were retrieved from the Web of Science Core Collection database. Microsoft Excel 2021 was used to analyze the main findings. CiteSpace, VOSviewer, and a scientometric online platform were used to perform visualization analysis of the co-citation of journals and references, co-occurrence of keywords, and collaboration between countries/regions, institutions, and authors.Results: A total of 1755 publications were finally included, which were published by 795 authors in 443 institutions from 59 countries/regions. The United States was the top country in terms of the number and centrality of publications in this domain, with 810 papers and a centrality of 0.38, and Harvard University published the largest number of articles (182). Fayad, ZA was the most productive author, with 73 papers, while LIBBY P had the most co-citations (493). CIRCULATION was the top co-cited journal with a frequency of 1,411, followed by ARTERIOSCL THROM VAS (1,128). The co-citation references analysis identified eight clusters with a well-structured network (Q = 0.6439) and highly convincing clustering (S = 0.8865). All the studies calculated by keyword co-occurrence were divided into five clusters: “nanoparticle,” “magnetic resonance imaging,” “inflammation,” “positron emission tomography,” and “ultrasonography”. Hot topics mainly focused on cardiovascular disease, contrast media, macrophage, vulnerable plaque, and microbubbles. Sodium fluoride ⁃PET, targeted drug delivery, OCT, photoacoustic imaging, ROS, and oxidative stress were identified as the potential trends.Conclusion: Molecular imaging research in atherosclerosis has attracted extensive attention in academia, while the challenges of clinical transformation faced in this field have been described in this review. The findings of the present research can inform funding agencies and researchers toward future directions

COVID-19 vaccine-related misinformation identification among Chinese residents during a regional outbreak

ObjectivesMisinformation about the COVID vaccines poses a significant challenge to vaccination efforts in many countries. This study examined Chinese citizens’ ability to correctly identify COVID-19 vaccine misinformation in geographic areas with and without a regional outbreak. We also investigated the associations between misinformation identification and information source usage, source trust, perceived information quality, and demographic characteristics.SettingThe online survey was conducted in four cities from June 8th to 15th, 2021 in Guangdong Province, two of which were experiencing a regional surge of COVID-19 delta variant infections, and four cities in Hunan Province, a neighboring province largely unaffected.ParticipantsA total of 4,479 individuals aged 18 and above completed the online questionnaire. Given survey length, those who finished the study under 5 min were excluded, resulting in a final sample of 3,800.Outcome measurementsMisinformation identification, source exposure, source trust, and perceived information quality.ResultsResults showed slightly higher levels of correct misinformation identification in surge vs. non-surge areas. Trust in official information sources was positively associated with correct misinformation identification in full sample analysis, while trust in informal sources was negatively associated with the same outcome. Perceived information quality was positively associated with correct misinformation identification in the full sample.ConclusionInformation providers in China should enhance the quality of the vaccine information they provide, and the Chinese public should balance their usage of different sources of information to acquire vaccine knowledge