2 research outputs found
<i>N</i>‑Phenyl-2-naphthylamine as a Novel MALDI Matrix for Analysis and in Situ Imaging of Small Molecules
Due
to its strong ultraviolet absorption, low background interference
in the small molecular range, and salt tolerance capacity, <i>N</i>-phenyl-2-naphthylamine (PNA) was developed as a novel
matrix in the present study for analysis and imaging of small molecules
by matrix-assisted laser desorption/ionization mass spectrometry time-of-fight
(MALDI-TOF MS). The newly developed matrix displayed good performance
in analysis of a wide range of small-molecule metabolites including
free fatty acids, amino acids, peptides, antioxidants, and phospholipids.
In addition, PNA-assisted LDI MS imaging of small molecules in brain
tissue of rats subjected to middle cerebral artery occlusion (MCAO)
revealed unique distributions and changes of 89 small-molecule metabolites
including amino acids, antioxidants, free fatty acids, phospholipids,
and sphingolipids in brain tissue 24 h postsurgery. Fifty-nine of
the altered metabolites were identified, and all the changed metabolites
were subject to relative quantitation and statistical analysis. The
newly developed matrix has great potential application in the field
of biomedical research
In Situ Bioconjugation and Ambient Surface Modification Using Reactive Charged Droplets
Molecular ions are generated in induced
electrospray ionization,
and they can be transported to grounded ambient surfaces in the form
of charged microdroplets. Efficient amide bonds formation between
amines and carboxylic acids were observed inside charged droplets
during transfer to the surface. Biomolecules derivatized using this
method were self-assembled on a bare gold surface via Au–S
bonds under the charged microdroplet environment. Cyclic voltammetric
analysis of the self-assembled molecular film showed accelerated protein
derivatization with cysteine, which allowed the covalent immobilization
of the protein to the gold surface. Cytochrome C-functionalized electrodes
prepared using the induced dual nanoelectrospray process showed bioactivity
toward aqueous solutions of hydrogen peroxide below 50 μM. In
effect, we have developed a method that allows derivatization of biomolecules
and their immobilization at ambient surfaces in a single experimental
step