Catalase and superoxide dismutase enzymes activities in Flaxseed (Linum Usitatissimum) extract

In this study, catalase (E.C.1.11.1.6) and superoxide dismutase (EC 1.15.1.1) activities were determined in the extract of flaxseed (Linum usitatissimum) with high antioxidant effect. Activities were determined after homogenization, ultracentrifugation and PD-10 (Sephadex G-25M) column chromotography steps. After the ultracentrifugation step, catalase and superoxide dismutase specific activities were found as 15.94 U/mg prot. and 2.05 U/mg prot., respectively. After desalting with PD-10 column chromotography, catalase and superoxide dismutase specific activities were found as 17.56 U/mg prot. and 4.90 U/mg prot., respectively

Determination of superoxide dismutase (SOD), Ggutathione peroxidase (GSH-Px) activities and MDA levels in the erythrocytes treated with H2O2 in the presence and absence of 2,6-diisopropyl phenol

30th Congress of the Federation-of-European-Biochemical-Societies (FEBS)/9th IUBMB Conference -- JUL 02-07, 2005 -- Budapest, HUNGARYWOS: 000234826102295…Federat European Biochem Soc, Int Union Biochem & Mol Bio

Purification of soybean lipoxygenase

30th Congress of the Federation-of-European-Biochemical-Societies (FEBS)/9th IUBMB Conference -- JUL 02-07, 2005 -- Budapest, HUNGARYWOS: 000234826102530…Federat European Biochem Soc, Int Union Biochem & Mol Bio

Serum malondialdehyde level in patients with cystic echinococcosis

PubMedID: 17106545Objective: To investigate the changes of serum malondialdehyde (MDA) level for the oxidative stress hypothesis in patients with cystic echinococcosis (CE). Methods: The study was conducted on patients with CE before the surgical treatment. Anti-Echinococcus granulosus antibodies were determined by serological method. We assayed MDA activities measured of 57 subjects and matched in 45 healthy control. This study took place in the Faculty of Medicine, University of Cukurova, Balcali Hospital in Adana, Turkey, between March 2004 and October 2005. Results: The mean ± SD of MDA levels of patients with CE was 6.70 ± 1.66 and healthy controls was 2.53 ± 0.43. The difference between MDA levels of patients and controls was statistically significant (p<0.001). Conclusion: The high infection/control ratio of MDA concentration and the significant correlation strongly indicate the occurrence of oxidative stress and lipid peroxidation as a mechanism of tissue damage in cases of CE. Our study highlights the usefulness of serum markers to investigate complex pathological situations, including distinct forms of chronic liver inflammation associated with CE

The effect of lead on sera malonyldialdehyde, vitamin C, vitamin E and erythrocyte reduced glutathione

Lead is one of the heavy metals that is toxic for human and animals. Several reports indicate that peroxidation damage of tissues in laboratory animals are enhanced in acute toxicity from transition metal ions such as copper, iron, mercury and lead. In this study sera malondialdehyde (MDA), erythrocyte reduced glutathione(GSH), sero vitamin C and E values were determined in 30 male subjects occupationally exposed to lead 20 healthy male subjects were used as control group. Blood lead level of control group was found as 10 ± 1.8 mg/dL. However, blood lead level of lead exposed subjects was very high with a value of 317 ± 47 mg/dL. MDA, GSH, vitamin C and vitamin E levels of lead exposed subjects were 0.56 ± 0.30 nmol/mL, 2.77 ± 0.50 mmol/g Hb, 1.33 ± 0.40 mg/dL and 5.40 ± 0.60 mg/mL, respectively. MDA, GSH, vitamin C and vitamin E value of control group were 0.17 ± 0.02 nmol/mL, 5.10 ± 1.20 mmol/g Hb, 1.75 ± 0.34 mg/dl and 8.98 ± 1.75 mg/mL, respectively. Our experimental results showed that there is a significant increase in MDA values of lead exposed subjects as compared with that of the controls. However, vitamin E, vitamin C and GSH values of lead exposed subjects were significantly lower than the vitamin E, vitamin C and GSH values of normal controls

Oxidative stress in vivax malaria

PubMedID: 23230340Malaria is still a leading cause of morbidity and mortality. The increase in lipid peroxidation reported in malaria infection and antioxidant status may be a useful marker of oxidative stress during malaria infection. The aim of this study was to investigate the role of antioxidant enzymes against toxic reactive oxygen species in patients infected with Plasmodium vivax and healthy controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were determined in 91 P. vivax patients and compared with 52 controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were 8.07 ±2.29 nM/ml, 2.69±0.33 U/ml, and 49.6±3.2 U/g Hb in the patient group and 2.72 ± 0.50 nM/ml, 3.71 ± 0.47 U/ml, and 62.3 ±4.3 U/g Hb in the control group, respectively. Malondialdehyde levels were found statistically significant in patients with vivax malaria higher than in healthy controls (P< 0.001). On the other hand, superoxide dismutase and glutathione peroxidase activities were found to be signifcantly lower in vivax malaria patients than in controls (P<0.05). There was an increase in oxidative stress in vivax malaria. The results suggested that antioxidant defense mechanisms may play an important role in the pathogenesis of P. vivax. © 2012, Korean Society for Parasitology and Tropical Medicine

Zinc and selenium status of healthy children from the Central Anatolian region of Turkey

The concentrations of zinc in serum and hair from more than 600 healthy children aged between 7-12 years living in Konya (a city in the Central Anatolian region of Turkey) were measured to determine if their dietary zinc intakes and general zinc status are satisfactory. Their body weights and heights were also measured together with assessments of socioeconomic status and food consumption. There was a positive association between physical development, shown as larger height-for-age percentiles, and zinc concentrations in serum and hair both in females and males. The socioeconomic status of the children was strongly associated with levels of zinc in these samples and the lowest concentrations of zinc were found in children from the families with poor incomes and in children who rarely consumed meat. Serum selenium concentrations were similar to those found in Adana (Southern Turkey) and indicate that no active intervention to regulate selenium status is necessary

Paraoxonase1 gene polymorphisms and serum paraoxonase activity in Turkish non-Hodgkin's lymphoma patients

40th Congress of the Federation-of-European-Biochemical-Societies (FEBS) - The Biochemical Basis of Life -- JUL 04-09, 2015 -- Berlin, GERMANYWOS: 000362570601104…Federat European Biochemical So

Antioxidant enzyme status in human cystic echinococcosis

The objective of this study to investigate the role of antioxidant enzymes against toxic reactive oxygen species in human Hydatid disease (HD). This study took place in the Faculty of Medicine, University of Cukurova, Balcali Hospital in Adana, Turkey, from March 2004 to October 2005. The study was conducted on patients with cyctic echinococcosis (CE) before the surgical treatment and compared with healthy controls. Cyctic echinococcosis was determined by ELISA and western blotting method in serum samples. We assayed catalase (CAT) and superoxide dismutase activities (SOD) measured of 28 subjects and matched in 45 healthy controls. CAT and SOD activities of the patients and control group were found as 15.18 × 104 ± 3.25 ± 104 lU/mg Hb, 2.40 ± 0.27 U/mL and 17.84 × 104 ± 2.61 × 104 IU/mg Hb, 3.63 ± 0.41 U/mL, respectively. Present results showed that there was a significant decrease in CAT (p &lt; 0.05) and SOD (p &lt; 0.001) activities of patients group as compared with that of the controls. There is an increase in oxidative stress in cystic echinococcosis. Despite this stress, the antioxidant system is deficient and adequate, in patients with cystic echinococcosis could be a defense system promotes the regulation and expression of these enzymes

Zinc and selenium status of healthy children from the Central Anatolian region of Turkey

The concentrations of zinc in serum and hair from more than 600 healthy children aged between 7 - 12 years living in Konya (a city in the Central Anatolian region of Turkey) were measured to determine if their dietary zinc intakes and general zinc status are satisfactory. Their body weights and heights were also measured together with assessments of socioeconomic status and food consumption. There was a positive association between physical development, shown as larger height-for-age percentiles, and zinc concentrations in serum and hair both in females and males. The socioeconomic status of the children was strongly associated with levels of zinc in these samples and the lowest concentrations of zinc were found in children from the families with poor incomes and in children who rarely consumed meat, Serum selenium concentrations were similar to those found in Adana (Southern Turkey) and indicate that no active intervention to regulate selenium status is necessary