Multiple parton scattering and gluon saturation in dijet production at EIC

Large angle gluon radiations induced by multiple parton scatterings contribute to dijet production in deeply inelastic scattering off a large nucleus at the Electron-Ion Collider. Within the generalized high-twist approach to multiple parton scattering, such contributions at the leading order in perturbative QCD and large Bjorken momentum fraction $x_B$ can be expressed as a convolution of the multiple parton scattering amplitudes and the transverse momentum dependent (TMD) two-parton correlation matrix elements. We study this medium-induced dijet spectrum and its azimuthal angle correlation under the approximation of small longitudinal momentum transfer in the secondary scattering and the factorization of two-parton correlation matrix elements as a product of quark and gluon TMD parton distribution function (PDF). Using a simple model for gluon saturation based on the parametrized gluon TMD PDF, we can calculate the $x_B$ and $Q^2$ dependence of the saturation scale and parton transport coefficient $\hat q$. Contributions to dijet cross section from double scattering are power-suppressed and only become sizable for mini-jets at small transverse momentum. We find that the total dijet correlation for these mini-jets, which also includes the contribution from single scattering, is sensitive to the transverse momentum broadening in the quark TMD PDF at large $x$ and saturation in the gluon TMD PDF at small $x$ inside the nucleus. The correlation from double scattering is also found to increase with the dijet rapidity gap and have a quadratic nuclear-size dependence because of the Landau-Pomeranchuk-Migdal (LPM) interference in gluon emission induced by multiple scattering. Experimental measurements of such unique features in the dijet correlation can shed light on the LPM interference in strong interaction and gluon saturation in large nuclei.Comment: 23 pages in RevTex with 21 figure

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

A Wavelet-Based Computational Framework for a Block-Structured Markov Chain with a Continuous Phase Variable

We consider the computing issues of the steady probabilities for block-structured discrete-time Markov chains that are of upper-Hessenberg or lower-Hessenberg transition kernels with a continuous phase set. An effective computational framework is proposed based on the wavelet transform, which extends and modifies the arguments in the literature for quasi-birth-death (QBD) processes. A numerical procedure is developed for computing the steady probabilities based on the fast discrete wavelet transform, and several examples are presented to illustrate its effectiveness

Clinical Significance of MiR-137 Expression in Patients with Gastric Cancer After Radical Gastrectomy.

The dysregulation of miR-137 plays vital roles in the oncogenesis and progression of various types of cancer, but its role in prognosis of gastric cancer patients remains unknown. This study was designed to investigate the expression and prognostic significance of miR-137 in gastric cancer patients after radical gastrectomy. Quantitative real-time PCR (qRT-PCR) was performed to evaluate the expression of miR-137 in human gastric cancer cell lines and tissues in patients with gastric adenocarcinoma. Results were assessed for association with clinical factors and overall survival by using Kaplan-Meier analysis. Prognostic values of miR-137 expression and clinical outcomes were evaluated by Cox regression analysis. The results exhibited that the expression level of miR-137 was decreased in human gastric cancer cell lines and tissues, and down-regulated expression of miR-137 was associated with tumor cell differentiation, N stage, and TNM stage. Decreased miR-137 expression in gastric cancer tissues was positively correlated with poor overall survival of gastric cancer patients. Further multivariate Cox regression analysis suggested that miR-137 expression was an independent prognostic indicator for gastric cancer except for TNM stage. Applying the prognostic value of miR-137 expression to TNM stage III group showed a better risk stratification for overall survival. In conclusion, the results reinforced the critical role for the down-regulated miR-137 expression in gastric cancer and suggested that miR-137 expression could be a prognostic indicator for this disease. In addition, these patients with TNM stage III gastric cancer and low miR-137 expression might need more aggressive postoperative treatment and closer follow-up

Multivariate Cox Regression Analyses for Overall Survival.

<p>Abbreviation: 95% CI, 95% confidence interval.</p><p>Multivariate Cox Regression Analyses for Overall Survival.</p

The expression of miR-137 in human gastric cells and tissues.

<p>(A) qRT-PCR analysis for miR-137 expression levels in immortalized normal human gastric epithelial cell line GES-1 and five human gastric cancer cell lines (MKN-45, SGC7901, BGC-823, MGC-803, and AGS). (B-C) The percent of patients with low miR-137 expression increased accompanied with disease progression from TNM stage I to III in (B) Training set (n = 67) and (C) Validation set (n = 87). *P<0.05.</p

FMDV Leader Protein Interacts with the NACHT and LRR Domains of NLRP3 to Promote IL-1β Production

Foot-and-mouth disease virus (FMDV) infection causes inflammatory clinical symptoms, such as high fever and vesicular lesions, even death of animals. Interleukin-1β (IL-1β) is an inflammatory cytokine that plays an essential role in inflammatory responses against viral infection. The viruses have developed multiple strategies to induce the inflammatory responses, including regulation of IL-1β production. However, the molecular mechanism underlying the induction of IL-1β by FMDV remains not fully understood. Here, we found that FMDV robustly induced IL-1β production in macrophages and pigs. Infection of Casp-1 inhibitor-treated cells and NOD-, LRR- and pyrin domain-containing 3 (NLRP3)-knockdown cells indicated that NLRP3 is essential for FMDV-induced IL-1β secretion. More importantly, we found that FMDV Lpro associates with the NACHT and LRR domains of NLRP3 to promote NLRP3 inflammasome assembly and IL-1β secretion. Moreover, FMDV Lpro induces calcium influx and potassium efflux, which trigger NLRP3 activation. Our data revealed the mechanism underlying the activation of the NLRP3 inflammasome after FMDV Lpro expression, thus providing insights for the control of FMDV infection-induced inflammation

Univariate Cox Regression Analyses for Overall Survival.

<p>Abbreviation: 95% CI, 95% confidence interval.</p><p>^†Split at median.</p><p>Univariate Cox Regression Analyses for Overall Survival.</p

Analyses of overall survival according to the expression of miR-137 in gastric cancer patients.

<p>Kaplan-Meier analyses of overall survival according to miR-137 expression in (A) Training set (n = 67) and (B) Validation set (n = 87). P value was calculated by log-rank test.</p