CURCUMINOIDS ANALYSIS IN CURCUMA MANGGA RHIZOMES

Objective: The current study was conducted to analyze the curcuminoid content in ethanol extract of Curcuma mangga. Methods: The constituent of ethanol extract of C. mangga was determined using ultra-performance liquid chromatography (UPLC). The diluted solutions of the extracts and the reference standards were analyzed separately by the reversed-phase UPLC method with mobile phase of acetonitrile: water (40:60) isocratically eluted for 30 min.Results: The chromatograms of the ethanol extract of C. mangga showed the presence of curcumin, bisdemethoxycurcumin, and demethoxycurcumin (DMC). Of all the curcuminoids content, DMC was the major component, corresponding to retention time at 9.96 min as compared to curcuminoid standard at 9.69 min.Conclusion: The results indicate that ethanol extract of C. mangga rhizomes contains DMC at the highest amount as compared to other curcuminoid compounds

ANTIHYPERURICEMIC ACTIVITY OF ETHANOL EXTRACT OF SYZYGIUM CUMINI LEAVES ON POTASSIUM OXONATED-INDUCED RATS

Objective: This study was conducted to investigate the effect of extract of Syzygium cumini on serum uric acid levels of rats induced by potassium oxonate.Methods: A total of 30 male Wistar rats were randomly divided into six groups, consist of one normal group (without treatment) and five groups that were induced by potassium oxonate 250 mg/kg bw intraperitoneally for 7 days. The ethanol extract of S. cumini leaves dose (100, 200, and 400 mg/kg bw) was administered orally 1 h after the potassium oxonate exposure during 7 days. On day 8, blood samples were collected for serum uric acid determination.Result: Ethanol extract of S. cumini leaves at doses of 100 mg/kg bw, 200 mg/kg bw, and 400 mg/kg bw significantly reduced serum uric acid as compared to negative control group of carboxymethylcellulose 0.5%.Conclusion: Ethanol extract of S. cumini leaves can be used as antihyperuricemic agent

ANTIDIABETIC ACTIVITY FROM ETHANOL EXTRACT OF KLUWIH’S LEAF (Artocarpus camansi)

Research on antidiabetic activity of ethanol leaf extracts Kluwih (Artocarpus camansi) has been carried out to determine the antidiabetic activity of ethanol extract of leaves kluwih (EELK) of mice using glucose tolerance test. The study began by dividing the mice into 6 groups. Group 1 was given 1% CMC, group 2 to group 4, given EELK 50, 100, 200, 400 mg / kg bw, respectively, and group 6  administered with glibenclamide 0.45 mg/kg bw. Thirty minutes later each group was given glucose 30 mg/kg bw. After 30 min of glucose loading, blood glucose levels of mice were measured, and measured again at minute 60, 90, 120 and 150 minutes. The results showed that the glucose tolerance test EELK a significant effect in lowering blood glucose levels compared to controls at 30 and 60 minutes after administration of glucose. At minute 90 to 150, the effect of blood glucose levels EELK restore back to normal. EELK demonstrated ability to reduce blood glucose levels, and EELK doses of 50 and 100 mg/kg bw showed antidiabetic effects better than a dose of 200 and 400 mg/kg bw. Increasing doses showed antagonistic effects: the ability to increase blood glucose levels

IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT ARTEMISIA VULGARIS L. IN MALE RATS

  Objective: This current study was conducted to investigate the immunomodulatory effects of ethanol extract of Artemisia vulgaris in male rats.Methods: Immunomodulatory activity was determined by measuring the carbon elimination rate, the total number of leukocytes and differential, as well as antibody titer. The treatment groups were divided into six groups, each group consisting of five male rats. The extract at doses of 50, 100, 200, and 400 mg/kg bw was administered orally for 7 days. The carbon elimination was measured using an ultraviolet-visible spectrophotometer.Results: The ethanol extract of A. vulgaris at doses of 50, 100, 200, and 400 mg/kg body weight increased the carbon elimination and increased the number of total leukocytes and differential leukocytes, especially neutrophils and neutrophils rod segments as compared to the negative control group (CMC-Na 0.5%) (p<0.05).Conclusion: The ethanol extract of A. vulgaris possesses immunomodulatory effect

Antidiabetic Activity From Ethanol Extract of Kluwih's Leaf (Artocarpus Camansi)

Research on antidiabetic activity of ethanol leaf extracts Kluwih (Artocarpus camansi) has been carried out to determine the antidiabetic activity of ethanol extract of leaves kluwih (EELK) of mice using glucose tolerance test. The study began by dividing the mice into 6 groups. Group 1 was given 1% CMC, group 2 to group 4, given EELK 50, 100, 200, 400 mg / kg bw, respectively, and group 6 administered with glibenclamide 0.45 mg/kg bw. Thirty minutes later each group was given glucose 30 mg/kg bw. After 30 min of glucose loading, blood glucose levels of mice were measured, and measured again at minute 60, 90, 120 and 150 minutes. The results showed that the glucose tolerance test EELK a significant effect in lowering blood glucose levels compared to controls at 30 and 60 minutes after administration of glucose. At minute 90 to 150, the effect of blood glucose levels EELK restore back to normal. EELK demonstrated ability to reduce blood glucose levels, and EELK doses of 50 and 100 mg/kg bw showed antidiabetic effects better than a dose of 200 and 400 mg/kg bw. Increasing doses showed antagonistic effects: the ability to increase blood glucose levels

NEPHROPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF CURCUMA MANGGA VAL. IN PARACETAMOL-INDUCED MALE MICE

Objective: This study aimed to evaluate nephroprotective activity of Curcuma mangga in paracetamol-induced male mice.Methods: Male mice were divided into several groups including normal control, negative, positive, and treatment groups. Treatment groups were orally administered with C. mangga extract at doses of 100, 200, and 400 mg/kg bw for 7 days. On day 7after 1 h of treatment, the mice were induced with paracetamol 1.05 g/kg bw. Serum creatinine level measurement and histopathotlogy study were performed at the end of experiment.Results: C. mangga extract was able to inhibit the increase of creatinine level and showed a significantly different from negative control (p<0.05) and did not different significantly from positive control (p>0.05). The result was supported by histopathology examination which did not show any cell damage. The nephroprotective effect of C. mangga was in a dose-dependent manner. C. mangga extract at dose of 400 mg/kg bw depicted the strongest nephroprotective effect.Conclusion: C. mangga extract was able to protect mice kidney induced by paracetamol

IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT OF CURCUMA MANGGA RHIZOMES IN MICE

Objective: This study was conducted to evaluate the immunomodulatory effects of ethanol extract of Curcuma mangga by in vivo study.Methods: The ethanol extract of C. mangga was comprised to carbon clearance method for its immunomodulatory potential. The extract wasadministered orally at doses of 100, 200, and 400 mg/kg BW to mice for 7 days. On day 8, carbon ink was injected, and the blood was collected formeasurement of elimination of carbon. Total leukocyte count was also determined.Results: The evaluation of immunomodulatory potential of ethanol extract of C. mangga revealed a dose-dependent increase in phagocytosis ability.The phagocytic index of ethanol extract of C. mangga was more than those of negative control, indicating the immunostimulatory activity of C. mangga.It showed low stimulation on total leukocyte count.Conclusion: The results indicate that ethanol extract of C. mangga rhizomes possesses immunomodulatory activity and has therapeutic potential forthe treatment of infectious diseases

The Inhibitory Activity of Picria fel-terrae Lour Herbs Extract on Nitric Oxide Production toward RAW 264.7 Cells Induced by Lipopolysaccharide

AIM: The objective of this study was to evaluate the inhibitory activity of Picria fel-terrae Lour on Nitric Oxide production toward RAW 264.7 cells. METHODS: The extraction was obtained by maceration method using n-hexane, ethyl acetate and ethanol solvents and then nitric oxide (NO) production was obtained using Griess reagent. RESULTS: Extract of Picria fel-terrae Lour herbs can reduce the NO production toward RAW 264.7 cells with induced by lipopolysaccharide has obtained nitric concentrations 12.5 and 25 μg/mL from n-hexane extract (72.50 ± 4.51 and 10.42 ± 1.82), ethyl acetate extract: (88.33 ± 6.51 and 30.83 ± 6.86), ethanol extract: (75.00 ± 1.91 and 22.08 ± 2.53). CONCLUSION: n-hexane extract of Picria fel-terrae Lour Herbs has a high potential to reduce the NO production in LPS-stimulated RAW 264.7 cells compared to ethyl acetate and ethanol extracts of Picria fel-terrae Lour Herbs

Evaluation of Waiting Time for Outpatient Prescription Services at the Pharmacy Installation at the Universitas Sumatera Utara Hospital

The pharmacy installation is one of the units in the hospital that provides. service products and services in the form of prescription services. Prescription service asthe front line of pharmacy services to patients must be managed properly becausethe quality of pharmaceutical prescription service which is generally associated with the speed ingiving service. This research is a descriptive type of research with prospective data collection from March-June 2019 to evaluate the waiting time for outpatient prescription services at the Hospital Pharmacy Installation of the Universitas Sumatera Utara. The data taken were 357 outpatient prescriptions.The results of this study indicate the total average length of time waiting for outpatients for the provision of non-fake drugs at the Pharmacy Installation of Outpatients at the University of Sumatera Utara Hospital at 09.00 - 11.00 is 29.10 minutes, at 11.00 - 13.00 is 34.44. minutes and at 13.00 - finished 42.60 minutes. The average length of waiting time for outpatients for the provision of compound drugs at the Outpatient Pharmacy Installation of the University of Sumatera Utara Hospital at 09.00 - 11.00 is 51.67 minutes, 11.00 - 13.00 hours is 62.27 minutes and at 13.00 - completion was 65.71 minutes. The waiting time for prescription services is ≤ 60 minutes.Based on the results of research conducted at the Outpatient Pharmacy Installation at the University of Sumatera Utara Hospital, non-concocted prescription drugs meet the requirements if the service time speed is ≤ 30 minutes and the concocted drug prescription fulfills the requirements if the service time speed is ≤ 60 minutes

Hepatoprotective Activity of Curcuma mangga Extract on Paracetamol-Induced Male Mice

This study was carried out to investigate the protective effect of ethanol extract of Curcuma mangga rhizomes on paracetamol-induced hepatotoxicity. High dose of paracetamol (1.35g/kg bw) was used to induce hepatic necrosis of mice liver. The male mice  received ethanol extract of C. mangga rhizomes (100, 200 and 400 mg/kg BW) for 7 days. The hepatoprotective actvity of extract was compared to normal, positive (curcuma) and negative control. The liver function was evaluated by measuring the biochemistry parameters which include alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, histophatological study on hepatic tissue section was also carried out. The C. mangga extract displayed hepatoprotective effect except at dose of 100 mg/kg bw. The increasing of serum levels of AST and ALT were inhibited after treatment with ethanol extract at doses of 200 and 400 mg/kb bw which was comparable with normal and curcuma as postive control (p>0.05). In addition, histological assessment of hepatic tissue demonstrated no liver damage, specially at dose of 400 mg/kb BW. The result indicate that ethanol extract of C. mangga rhizomes has hepatoprotective effect, especially at doses of 200 and 400 mg/kg bw .   Keywords: C. mangga, rhizomes, biochemistry parameters, histopatholog