Analisis Penentuan Kualitas Air Dan Status Mutu Sungai Progo Hulu Kabupaten Temanggung

Progo Hulu River is the main river in Progo watershed, Temanggung Regency. It was estimated that human activities have some important contributions in its water quality degradation, for example housings/settlement, agricultural, industry, etc. This research was aimed to analize Progo Hulu River which focused on (1) river water quality, (2) some factors that cause the different effects of each segment concentration, and (3) water quality status for every segment. The river water quality was observed in 12 sampling-points and then it compared with water quality standart according to the Government Act No. 82/2001. This research also describes water quality status used STORET and Pollution Index method based on Ministry Decree No. 115/2003. As result, Fecal Coliform become a dominant parameter that caused pollution in first class water quality, whereas Ammonia and Fecal Coliform caused pollution in second, third, and fourth class water quality with a main pollution source from domestic and ranch wastes. Besides, water quality status showed it has been modaretely polluted in first class water quality status and lightly- modaretely polluted in second, third, and fourth class water quality status

Additional file 1 of The association between trimethylamine N-oxide levels and ischemic stroke occurrence: a meta-analysis and Mendelian randomization study

Additional file 1: Table S1. Search stratages for literatures applied in the meta-analysis. Table S2. Genetic IVs for TMAO. Table S3. Estimation of Wald ratio between each SNP related to TMAO and risk of IS. Table S4. Heterogeneity analysis using Cochran’s Q test. Table S5. Horizontal pleiotropy analysis using the intercept of MR-Egger regression test

Additional file 4: Figure S4. of Physiological and proteomic analyses of the drought stress response in Amygdalus Mira (Koehne) Yü et Lu roots

Semi-quantitative PCR analysis of eleven genes and ACTIN of Amygdalus mira (Koehne) Yü et Lu roots during drought stress and recovery period, respectively. (DOC 201 kb

Planar Chiral Ferrocene Cyclopalladated Derivatives Induce Caspase-Dependent Apoptosis and Antimetastasis in Cancer Cells

A series of planar chiral ferrocene cyclopalladated compounds were synthesized and characterized. The absolute configurations of four compounds were determined by single-crystal X-ray analysis. The cytotoxic activities of the synthesized compounds and cisplatin exhibited different inhibition potencies on the viability of human liver, breast, and colon cancer cell lines. The dinuclear compound <b>7</b> was 16-fold more potent than cisplatin in hepatoma cells. Compound <b>7</b> was also more effective than cisplatin in the inhibition of hepatoma cell metastasis. Flow cytometry analysis and caspase activity assays indicated that compound <b>7</b> exerted antiproliferative activity in cancer cells through the induction of caspase-dependent apoptosis

Fluorescent Binary Ensemble Based on Pyrene Derivative and Sodium Dodecyl Sulfate Assemblies as a Chemical Tongue for Discriminating Metal Ions and Brand Water

Enormous effort has been put to the detection and recognition of various heavy metal ions due to their involvement in serious environmental pollution and many major diseases. The present work has developed a single fluorescent sensor ensemble that can distinguish and identify a variety of heavy metal ions. A pyrene-based fluorophore (<b>PB</b>) containing a metal ion receptor group was specially designed and synthesized. Anionic surfactant sodium dodecyl sulfate (SDS) assemblies can effectively adjust its fluorescence behavior. The selected binary ensemble based on <b>PB</b>/SDS assemblies can exhibit multiple emission bands and provide wavelength-based cross-reactive responses to a series of metal ions to realize pattern recognition ability. The combination of surfactant assembly modulation and the receptor for metal ions empowers the present sensor ensemble with strong discrimination power, which could well differentiate 13 metal ions, including Cu²⁺, Co²⁺, Ni²⁺, Cr³⁺, Hg²⁺, Fe³⁺, Zn²⁺, Cd²⁺, Al³⁺, Pb²⁺, Ca²⁺, Mg²⁺, and Ba²⁺. Moreover, this single sensing ensemble could be further applied for identifying different brands of drinking water

Discrimination of Metalloproteins by a Mini Sensor Array Based on Bispyrene Fluorophore/Surfactant Aggregate Ensembles

Fluorescent sensor arrays with pattern recognition ability have been widely used to detect and identify multiple chemically similar analytes. In the present work, two particular bispyrene fluorophores containing hydrophilic oligo­(oxyethylene) spacer, <b>6</b> and <b>4</b>, were synthesized, but one is with and the other is without cholesterol unit. Their ensembles with cationic surfactant (CTAB) assemblies realize multiple fluorescence responses to different metalloproteins, including hemoglobin, myoglobin, ferritin, cytochrome <i>c</i>, and alcohol dehydrogenase. The combination of fluorescence variation at monomer and excimer emission of the two binary sensor ensembles enables the mini sensor array to provide a specific fingerprint pattern to each metalloprotein. Linear discriminant analysis shows that the two-ensemble-sensor-based array could well discriminate the five tested metalloproteins. The present work realizes using a mini sensor array to accomplish discrimination of complex analytes like proteins. They also display a very high sensitivity to the tested metalloproteins with detection limits in the range of picomolar concentration

Using RNA interference to knock down expression of eEF1B subunits.

<p>Immunoblots of protein extracts from cell lines after RNA interference. Panel A: eEF1Bα, eEF1Bδ and eEF1Bγ protein level efficiently knocked down by three different siRNAs in HeLa cells 72 h after transfection. GAPDH was used as a loading control. Panel B: eEF1Bα, eEF1Bδ and eEF1Bγ protein level efficiently knocked down by three different siRNAs in HCT116 and DLD1 cells 72 h after transfection. GAPDH was used as a loading control.</p

Viability and cell cycle distribution of cells after ablation of eEF1B subunits.

<p>Panel A: A decrease in cellular metabolism is observed when any of the eEF1B subunit protein level is decreased by siRNAs in HeLa, HTC116 and DLD1 cells. Cell metabolism was assessed by the Alamar blue assay. Data were obtained from the mean of three or more independent experiments in HeLa, HCT116 and DLD1 cells, with more than 10 wells each. Error bars indicate +- SEM; n of wells>10; n = 3−4; *, P<0.05; ***, P<0.001 from non-targeting siRNA. Panel B: Knockdown of eEF1B subunits leads to altered cell cycle profile in three cell lines: representative images of the flow cytometry analysis. Error bars indicate +- SEM; n = 3; *, P<0.05; **, P<0.01 of non-targeting siRNA.</p

Expression in mouse spinal cord.

<p>IF images of the expression of eEF1A2 and eEF1Bα (top panel) or eEF1Bδ (bottom panel) on mouse spinal cord.</p