DEVELOPMENT OF A PILOT MODEL OF THE FRANCIS TURBINE FOR SMALL HYDROPOWER

This article discusses the problems of using alternative energy sources, hydraulic energy and the most common turbine — the Francis radial-axial turbine, designed for the needs of small hydropower.В статье рассматриваются проблемы использования альтернативных источников энергии, гидравлической энергии и самая распространенная турбина — радиально-осевая турбина Френсиса, спроектированная для нужд малой гидроэнергетики

Modeling and correction of hypoxia in laboratory animals

The article presents a study of the mechanisms of formation of artificial hypoxia in laboratory animals and correction of hypoxic conditions on etiotropic, pathogenetic and symptomatic principles.В статье представлено исследование механизмов формирования искусственной гипоксии у лабораторных животных и коррекция гипоксических состояний на этиотропном, патогенетическом и симптоматическом принципах

USE OF RENEWABLE ENERGY SOURCES FOR LIGHTING TRAILS

В работе рассмотрен вопрос возможности освещения автомобильных дорог от альтернативных источников энергии. Показана энергетическая, экономическая и экологическая целесообразность данного решения.The paper considers the possibility of lighting roads from alternative energy sources. The energy, economic and environmental feasibility of this solution is shown

Obstructive syndrome at children as a factor in the onset of bronchial asthma, differential diagnosis

The article presents a retrospective analysis of patients under the age of 10 years treated in the pulmonology department of Children's City Clinical Hospital № 11 with bronchial obstructive syndrome. Children at in the period from August to October 2019.В статье представлен ретроспективный анализ историй болезней пациентов в возрасте до 10 лет, пролеченных в пульмонологическом отделении ДГКБ № 11 с бронхообструктивным синдромом в период с августа по октябрь 2019 года

Voltammetric determination of organic ecotoxicants on modified electrodes

In this paper the contemporary state and the use of voltammetric methods for the determination of organic toxicants on the modified electrodes are shown. A review of works on the application of voltammetric methods for the analysis of environmental objects - water (natural, drinking, waste, etc.), published in the last 5 years, has been made. The main trends in electroanalytical control over the content of toxicants in the waters are noted. The experimental results on the expansion of determined organic toxicants are represented

Kinetic and Regulatory Properties of <i>Yarrowia lipolytica</i> Aconitate Hydratase as a Model-Indicator of Cell Redox State under pH Stress

This paper presents an analysis of the regulation activity of the partially purified preparations of cellular aconitate hydratase (AH) on the yeast Yarrowia lipolytica cultivated at extreme pH. As a result of purification, enzyme preparations were obtained from cells grown on media at pH 4.0, 5.5, and 9.0, purified by 48-, 46-, and 51-fold and having a specific activity of 0.43, 0.55 and 0.36 E/mg protein, respectively. The kinetic parameters of preparations from cells cultured at extreme pH demonstrated: (1) an increase in the affinity for citrate and isocitrate; and (2) a shift in the pH optima to the acidic and alkaline side in accordance with the modulation of the medium pH. The regulatory properties of the enzyme from cells subjected to alkaline stress showed increased sensitivity to Fe2+ ions and high peroxide resistance. Reduced glutathione (GSH) stimulated AH, while oxidized glutathione (GSSG) inhibited AH. A more pronounced effect of both GSH and GSSG was noted for the enzyme obtained from cells grown at pH 5.5. The data obtained provide new approaches to the use of Y. lipolytica as a model of eukaryotic cells demonstrating the development of a stress-induced pathology and to conducting a detailed analysis of enzymatic activity for its correction

Хондрогенне диференціювання мезенхімальних стовбурових клітин кроля, залежно від типу культуральної системи

Experimental study concerned with the ability of mesenchymal stem cells to chondrogenic differentiation in vitro depending on the type of culture system with a view to their further clinical use in veterinary medicine to restore the lost functions of damaged articular cartilage is one of the priority directions of scientific research. Reparative ability of cartilage tissue is limited, since the level of physiological and reparative regeneration of cartilage tissue is quite low. Given this, the search continues for such therapeutic agents and techniques that most effectively restore the damaged structure and function of the tissues of the articular cartilage. A special place in this search belongs to biotechnology. The bone marrow mesenchymal stem cells are one of the most studied multipotent populations of adult cells, which, due to their ability to proliferate and differentiate in vitro, is of great importance in clinical veterinary practice in treating animals with articular cartilage lesions. It was established that with the used protocol of chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells in the monolayer most of the cultured cells acquire a filamentous form, which leads to a decrease in the area of ​​their adhesion to the surface of the culture plastic and, as a result, detachment into the culture medium. During cultivation the confluence of cell monolayer in experimental cultures decreased and at 18 days of cultivation was about 5%. During cultivation the mesenchymal stem cells in the form of a precipitate in chondrogenic medium, for the 6th day of the study, it was visually discovered that the precipitated mass of cells at the bottom of the test tube became spherical, indicating cell compactification and are important condition for their differentiation in the chondrogenic direction. This form of micromass was maintained throughout the entire period of cultivation. Comparison of chondrogenic differentiation methods of rabbit bone marrow mesenchymal stem cells in monolayer and micromass showed that the method of differentiation of cells in the micromass is more efficient, since as early as 21 days during the study revealed significantly larger depositions of the extracellular matrix by cells compared to monolayer differentiation.Eкспериментальне вивчення здатності мезенхімальних стовбурових клітин до хондрогенного диференціювання in vitro залежно від типу культуральної системи з метою їх подальшого клінічного застосування у ветеринарній медицині для відновлення втрачених функцій пошкодженого суглобового хряща є одним із пріоритетних напрямків наукових досліджень. Репаративна здатність хрящової тканини обмежена, оскільки рівень фізіологічної та репаративної регенерації хрящової тканини досить низький. З огляду на це, триває пошук таких терапевтичних засобів та прийомів, які б дозволяли максимально ефективно відновити порушені структуру і функцію тканин суглобового хряща. Особливе місце у цьому пошуку належить біотехнології. Мезенхімальні стовбурові клітини кісткового мозку – одна з найбільш вивчених мультипотентних популяцій клітин дорослого організму, яка завдяки здатності до проліферації і диференціювання in vitro, має вагоме значення в клінічній ветеринарній практиці у лікуванні тварин з ураженнями суглобового хряща. Встановлено, що за апробованого протоколу хондрогенного диференціювання мезенхімальних стовбурових клітин кісткового мозку кроля у моношарі більшість культивованих клітин набуває ниткоподібної форми, що призводить до зниження площі їх адгезії до поверхні культурального пластику і, як наслідок, відкріплення у культуральне середовище. Порівняння методів хондрогенного диференціювання мезенхімальних стовбурових клітин кісткового мозку кроля у моношарі та мікромасі показало, що ефективнішим є метод диференціювання клітин у мікромасі, - вже на 21 добу дослідження виявлялись значно більші відкладання клітинами позаклітинного матриксу, порівняно з методом диференціювання у моношарі

Encapsulated Phytase Produced by Recombinant <i>Yarrowia lipolytica</i> Exhibits High Efficiency on Broiler Chickens in Low Dosage

Phytases are the largest group of feed enzymes increasing the accessibility of organic phosphorus for the animals. Feed phytases are usually sold as dried powder of secreting producers, mainly micellar fungi. We proposed a new technology for producing phytase from Obesumbacterium proteus (OPP) in yeast Yarrowia lipolytica as cytosolic protein (encapsulated OPP), where the capsule (yeast cell) protects the enzyme from unfavourable factors (acid medium and active proteolysis in stomachs) and releases it along with the substrate in the duodenum only. Here we report results of testing the encapsulated OPP on the model of a broiler chicken in comparison to a conventional phytase from Aspergillus ficuum. The encapsulated OPP at a dosage of 30 FYT/kg provided the maximum body weight of the chicken in the end of experiment equal or somewhat higher than in the control group, where the available phosphorus deficit was complemented with a mineral phosphorus supply. In contrast, the conventional soluble phytase at a dosage of 100 or 1000 FYT/kg was not able to compensate for the phosphorus deficit in the diet, although chemical analysis demonstrated much phosphorus in the diet in a non-accessible form. The encapsulated OPP decreased the residual Pi in the chicken faeces by 2.1 times in comparison to the control when added to the diet, whereas the conventional phytases negligibly affected this parameter regardless of the dosage