19,488 research outputs found
TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel
Transient receptor potential (TRP) channels, a superfamily of ion channels, can be divided into 7 subfamilies, including TRPV, TRPC, TRPP, and 4 others. Functional TRP channels are tetrameric complexes consisting of 4 pore-forming subunits. The purpose of this study was to explore the heteromerization of TRP subunits crossing different TRP subfamilies. Two-step coimmunoprecipitation (co-IP) and fluorescence resonance energy transfer (FRET) were used to determine the interaction of the different TRP subunits. Patch-clamp and cytosolic Ca2+ measurements were used to determine the functional role of the ion channels in flow conditions. The analysis demonstrated the formation of a heteromeric TRPV4-C1-P2 complex in primary cultured rat mesenteric artery endothelial cells (MAECs) and HEK293 cells that were cotransfected with TRPV4, TRPC1, and TRPP2. In functional experiments, pore-dead mutants for each of these 3 TRP isoforms nearly abolished the flow-induced cation currents and Ca2+ increase, suggesting that all 3 TRPs contribute to the ion permeation pore of the channels. We identified the first heteromeric TRP channels composed of subunits from 3 different TRP subfamilies. Functionally, this heteromeric TRPV4- C1-P2 channel mediates the flow-induced Ca2+ increase in native vascular endothelial cells.-Du, J., Ma, X., Shen, B., Huang, Y., Birnbaumer, L., Yao, X. TRPV4, TRPC1, and TRPP2 assemble to form a flowsensitive heteromeric channel.Fil: Du, Juan. Chinese University Of Hong Kong; Hong Kong. Anhui Medical University; ChinaFil: Ma, Xin. Chinese University Of Hong Kong; Hong KongFil: Shen, Bing. Chinese University Of Hong Kong; Hong Kong. Anhui Medical University; ChinaFil: Huang, Yu. Chinese University Of Hong Kong; Hong KongFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. National Institutes of Health; Estados UnidosFil: Yao, Xiaoqiang. Chinese University Of Hong Kong; Hong Kon
Liquid Density Sensing Using Resonant Flexural Plate Wave Device with Sol-Gel PZT Thin Films
This paper presents the design, fabrication and preliminary experimental
results of a flexure plate wave (FPW) resonator using sol-gel derived lead
zirconate titanates (PZT) thin films. The resonator adopts a two-port structure
with reflecting grates on the composite membrane of PZT and SiNx. The design of
the reflecting grate is derived from a SAW resonator model using COM theory to
produce a sharp resonant peak. The comparison between the mass and the
viscosity effects from the theoretical expression illustrates the applications
and the constraints of the proposed device in liquid sensing. Multiple coatings
of sol-gel derived PZT films are adopted because of the cost advantage and the
high electromechanical coupling effect over other piezoelectric films. The
fabrication issues of the proposed material structure are addressed.
Theoretical estimations of the mass and the viscosity effects are compared with
the experimental results. The resonant frequency has a good linear correlation
with the density of low viscosity liquids, which demonstrate the feasibility of
the proposed device.Comment: Submitted on behalf of EDA Publishing Association
(http://irevues.inist.fr/EDA-Publishing
Enhancing thermoelectric figure-of-merit by low-dimensional electrical transport in phonon-glass crystals
Low-dimensional electronic and glassy phononic transport are two important
ingredients of highly-efficient thermoelectric material, from which two
branches of the thermoelectric research emerge. One focuses on controlling
electronic transport in the low dimension, while the other on multiscale phonon
engineering in the bulk. Recent work has benefited much from combining these
two approaches, e.g., phonon engineering in low-dimensional materials. Here, we
propose to employ the low-dimensional electronic structure in bulk phonon-glass
crystal as an alternative way to increase the thermoelectric efficiency.
Through first-principles electronic structure calculation and classical
molecular dynamics simulation, we show that the - stacking
Bis-Dithienothiophene molecular crystal is a natural candidate for such an
approach. This is determined by the nature of its chemical bonding. Without any
optimization of the material parameter, we obtain a maximum room-temperature
figure of merit, , of at optimal doping, thus validating our idea.Comment: Nano Lett.201
Autobiographical design for emotional durability through digital transformable fashion and textiles
Research on Personalized Recommender System for Tourism Information Service
Since the development in the 1990s, Recommender system has been widely applied in various fields. The conflict between the expansion of tourism information and difficulty of tourists obtaining tourism information allows Tourism Information Recommender System to have a practical significance. Based on the existing online tourism information service and the mature recommendation algorithms, Personal Recommender System can be used to solve present problems of the key recommendation algorithms. In the first place, this research presents an overview of researches on this issue both at home and abroad, and analyzes the applications of main stream recommendation algorithms. Secondly, a comparative study of domestic and international tourism information service websites is conducted. Drawbacks in their applications are defined and advantages are adopted in the settings of Recommender System. Finally, this research provides the framework of Recommender System, which combines the design and test of algorithms and the existing tourism information recommendation websites. This system allows customers to broaden experience of tourism information service and make tourism decisions more accurately and rapidly. Keywords: Tourism information service, Personalized recommendation, Intelligence recommendation module, Apriori algorith
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