Methods for suicide-related behaviors and deaths by suicide.

<p>Methods for suicide-related behaviors and deaths by suicide.</p

Characteristics of people who exhibited suicide-related behaviors in Taiwan, 1997–2010.

<p>Characteristics of people who exhibited suicide-related behaviors in Taiwan, 1997–2010.</p

Supplemental Material - Prognostic Factors for Clinical Outcomes in Patients with Newly Diagnosed Advanced-stage Hodgkin Lymphoma: A Nationwide Retrospective Study

Supplemental Material Prognostic Factors for Clinical Outcomes in Patients with Newly Diagnosed Advanced-stage Hodgkin Lymphoma: A Nationwide Retrospective Study by Chieh-Lin Jerry Teng, Tran-Der Tan, Yun-Yi Pan, Yu-Wen Lin, Pei-Wen Lien, Hsin-Chun Chou, Peng-Hsu Chen, and Fang-Ju Lin in Cancer Control</p

Adjusted odds ratios for repeated suicide-related behaviors and deaths by suicide according to a logistic model (<i>n</i> = 57,874).

<p>Adjusted odds ratios for repeated suicide-related behaviors and deaths by suicide according to a logistic model (<i>n</i> = 57,874).</p

Application of flow cytometry for evaluating clinical prognosis and histopathological grade of human glioma

<p><b>Objectives:</b> Flow cytometry was applied to predict the biological parameters of tumor behavior based on the DNA content distribution of tumors. We used flow cytometry to determine the number of cell cycles for the characterization of intracranial gliomas and its possible prognostic role.</p> <p><b>Methods:</b> Flow cytometric analysis of the DNA content was performed for 37 fresh operative glioma specimens. The expression of Ki-67 in glioma specimens was detected using immunohistochemistry staining. The check points of G2/M-phase fractions, cyclin B, and pCdk1 (Y15) were analyzed using Western immunoblotting.</p> <p><b>Results:</b> Compared to low-grade (grade I/II) gliomas, significant differences in the Ki-67, cyclin B, G2/M-phase, and S+G2/M-phase expressions were found in high-grade (grade III/IV) gliomas. Furthermore, receiver operating characteristic (ROC) analysis indicated optimal cutoff points for the G2/M-phase and S+G2/M-phase fractions of 13.47 and 17.26%, respectively, which can be used to differentiate cases with low- and high-grade gliomas. Additionally, both G2/M-phase and S+G2/M-phase fractions had significant association with the expression of Ki-67 in the gliomas. The gliomas were classified by the DNA content. We found that patients with high-grade glioma had worse survival rate than patients with low-grade glioma. Meanwhile, ROC curve analysis gave cutoffs for G2/M-phase of 9.4% and for S+G2/M-phase fractions of 15.04% as best predicting survival. The patients with glioma had poor survival when the levels of G2/M-phase and S+G2/M-phase were more than 9.4 and 15.04%, respectively. In contrast, no significant association between the DNA content of glioma patients and their age, tumor recurrence, and tumor size was found.</p> <p><b>Discussion:</b> Our results indicate that flow cytometry analysis for G2/M-phase and S+G2/M-phase fractions can be used for tumor grading for rapidly differentiating low- from high-grade gliomas.</p

Additional file 1: of Incidence and risk factors for recurrent Henoch-Schรถnlein purpura in children from a 16-year nationwide database

Table S1. The generic name of steroid used in current cohort. (DOCX 133 kb

Increased basal K_ATP activity in both hetDel and hetT, del channels.

<p>⁸⁶Rb⁺ efflux of mutant Kir6.2 subunits coexpressed with WT subunits in 1∶1 DNA ratio, under metabolic inhibition (A) and in basal states (B). Data points indicate means ± SEM of n = 5. * indicates P<0.05 compared with WT by One-Way ANOVA analysis. NT  =  not transfected (no statistic given).</p

Expression of WT and homomeric mutant channels in patch clamp recordings.

<p>(A) representative currents recorded by inside-out excised patch-clamp technique from COSm6 cells expressing WT channels and various mutant channels, at +50 mV pipette potential. Patches were exposed to different concentrations of Mg-free ATP as indicated. Dashed line indicates zero current (WT and homS225T channels) or zero channel level (homDel and homT, del channels). (B) Western Blot of Flag-tagged SUR1 (fSUR1) from various constructs are indicated. The mature (cell surface) complex-glycosylated bands and immature core-glycosylated bands are indicated as upper bands (white arrow) and lower bands (black arrow) respectively. NT = not transfected.</p

Homology modeling of Kir6.2 from Kir2.2 structure with PyMOL software.

<p>(A) S225T is colored in yellow and deleted amino acids 226–232 (-PEGEVVP-) are colored in orange. (B) E227 and E229 are colored and labeled in red while R192 and R314 are colored and labeled in blue. The structure model reveals the possible interaction between the deleted amino acid P232 (orange spheres) and V319 in the proposed Kir6.2 Ankyrin-B binding site (a.a. 316 to a.a. 323 –VPIVAEED- colored in magenta).</p

Additional file 1: of Incidence and risk factors for recurrent Henoch-Schรถnlein purpura in children from a 16-year nationwide database

Table S1. The generic name of steroid used in current cohort. (DOCX 133 kb