<p>(A) representative currents recorded by inside-out excised patch-clamp technique from COSm6 cells expressing WT channels and various mutant channels, at +50 mV pipette potential. Patches were exposed to different concentrations of Mg-free ATP as indicated. Dashed line indicates zero current (WT and homS225T channels) or zero channel level (homDel and homT, del channels). (B) Western Blot of Flag-tagged SUR1 (fSUR1) from various constructs are indicated. The mature (cell surface) complex-glycosylated bands and immature core-glycosylated bands are indicated as upper bands (white arrow) and lower bands (black arrow) respectively. NT = not transfected.</p
