Increased electrical conductivity in fine-grained (Zr,Hf)NiSn based thermoelectric materials with nanoscale precipitates

Grain refinement has been conducted to reduce the thermal conductivity and improve the thermoelectric performance of the (Zr,Hf)NiSn based half-Heusler alloys. Nanoscale in situ precipitates were found embedded in the matrix with submicron grains. The lattice thermal conductivity was decreased due to the enhanced boundary scattering of phonons. The increased carrier concentration and electrical conductivity were observed compared to the coarse-grained alloys, which is discussed in relation to the existence of nanoscale precipitates, the effect of antisite defects, and composition change. It is suggested that the nanoscale precipitates play a significant role in the observed electrical conductivity increase

Confirming the 115.5-day periodicity in the X-ray light curve of ULX NGC 5408 X-1

The Swift/XRT light curve of the ultraluminous X-ray (ULX) source NGC 5408 X-1 was re-analyzed with two new numerical approaches, Weighted Wavelet $Z$-transform (WWZ) and CLEANest, that are different from previous studies. Both techniques detected a prominent periodicity with a time scale of $115.5\pm1.5$ days, in excellent agreement with the detection of the same periodicity first reported by Strohmayer (2009). Monte Carlo simulation was employed to test the statisiticak confidence of the 115.5-day periodicity, yielding a statistical significance of $> 99.98$ (or $>3.8\sigma$). The robust detection of the 115.5-day quasi-periodic oscillations (QPOs), if it is due to the orbital motion of the binary, would infer a mass of a few thousand $M_\odot$ for the central black hole, implying an intermediate-mass black hole in NGC 5408 X-1.Comment: 6 pages, 2 figures, submitted to Research in Astronomy and Astrophysics (RAA

Ionic effect on combing of single DNA molecules and observation of their force-induced melting by fluorescence microscopy

Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, we observed that the length of lambda-DNA molecules was extended to about 1.6 times their contour length (unextended length, 16.2 micrometers) by the combing method on hydrophobic polymethylmetacrylate (PMMA) coated surfaces. The effects of sodium and magnesium ions and pH of the DNA solution were investigated. Interestingly, we observed force-induced melting of single DNA molecules.Comment: 12 page

Bis[1,1′-(1,3-phenyl­enedimethyl­ene)di(1H-imidazol-3-ium)] β-octa­molybdate

In the title compound, (C14H16N4)2[Mo8O26], the β-octa­molybdate anion is centrosymmetric. N—H⋯O hydrogen bonds link the diimidazolium cations and the polyoxidoanions into a chain structure along [100]. π–π inter­actions between the imidazole rings and between the imidazole and benzene rings [centroid–centroid distances = 3.611 (2) and 3.689 (3) Å, respectively] connect the chains

Sequence homolog-based molecular engineering for shifting the enzymatic pH optimum

AbstractCell-free synthetic biology system organizes multiple enzymes (parts) from different sources to implement unnatural catalytic functions. Highly adaption between the catalytic parts is crucial for building up efficient artificial biosynthetic systems. Protein engineering is a powerful technology to tailor various enzymatic properties including catalytic efficiency, substrate specificity, temperature adaptation and even achieve new catalytic functions. However, altering enzymatic pH optimum still remains a challenging task. In this study, we proposed a novel sequence homolog-based protein engineering strategy for shifting the enzymatic pH optimum based on statistical analyses of sequence-function relationship data of enzyme family. By two statistical procedures, artificial neural networks (ANNs) and least absolute shrinkage and selection operator (Lasso), five amino acids in GH11 xylanase family were identified to be related to the evolution of enzymatic pH optimum. Site-directed mutagenesis of a thermophilic xylanase from Caldicellulosiruptor bescii revealed that four out of five mutations could alter the enzymatic pH optima toward acidic condition without compromising the catalytic activity and thermostability. Combination of the positive mutants resulted in the best mutant M31 that decreased its pH optimum for 1.5 units and showed increased catalytic activity at pH < 5.0 compared to the wild-type enzyme. Structure analysis revealed that all the mutations are distant from the active center, which may be difficult to be identified by conventional rational design strategy. Interestingly, the four mutation sites are clustered at a certain region of the enzyme, suggesting a potential “hot zone” for regulating the pH optima of xylanases. This study provides an efficient method of modulating enzymatic pH optima based on statistical sequence analyses, which can facilitate the design and optimization of suitable catalytic parts for the construction of complicated cell-free synthetic biology systems

Is tea consumption associated with the serum uric acid level, hyperuricemia or the risk of gout? A systematic review and meta-analysis

The methodological quality of cross-sectional studies in accordance with the Newcastle-Ottawa Scale (NOS). Table S2. The methodological quality of cohort studies in accordance with the Newcastle-Ottawa Scale (NOS). Table S3. The methodological quality of case–control studies in accordance with the Newcastle-Ottawa Scale (NOS). (DOCX 19 kb