4,806 research outputs found
Genetically engineered pre-microRNA-34a prodrug suppresses orthotopic osteosarcoma xenograft tumor growth via the induction of apoptosis and cell cycle arrest.
Osteosarcoma (OS) is the most common primary malignant bone tumor in children, and microRNA-34a (miR-34a) replacement therapy represents a new treatment strategy. This study was to define the effectiveness and safety profiles of a novel bioengineered miR-34a prodrug in orthotopic OS xenograft tumor mouse model. Highly purified pre-miR-34a prodrug significantly inhibited the proliferation of human 143B and MG-63 cells in a dose dependent manner and to much greater degrees than controls, which was attributed to induction of apoptosis and G2 cell cycle arrest. Inhibition of OS cell growth and invasion were associated with release of high levels of mature miR-34a from pre-miR-34a prodrug and consequently reduction of protein levels of many miR-34a target genes including SIRT1, BCL2, c-MET, and CDK6. Furthermore, intravenous administration of in vivo-jetPEI formulated miR-34a prodrug significantly reduced OS tumor growth in orthotopic xenograft mouse models. In addition, mouse blood chemistry profiles indicated that therapeutic doses of bioengineered miR-34a prodrug were well tolerated in these animals. The results demonstrated that bioengineered miR-34a prodrug was effective to control OS tumor growth which involved the induction of apoptosis and cell cycle arrest, supporting the development of bioengineered RNAs as a novel class of large molecule therapeutic agents
Effects of temperature on a Chinese population of Amblyseius andersoni (Acari: Phytoseiidae) fed with Tetranychus urticae
International audienceThe development and fecundity of Amblyseius andersoni (Chant) fed with Tetranychus urticae Koch was studied at five different temperatures (17, 20, 25, 30 and 35 °C) and life parameters of the population were calculated. The development, reproduction, longevity, and life table parameters of A. andersoni were significantly affected by the different temperatures. The duration of the egg, larval, protonymph, deutonymph and total immature stages were reduced when the temperature increased. The total oviposition of A. andersoni was highest at 25 °C and lowest at 35 °C, and the daily average oviposition increased as the temperature increased, but few eggs were laid at 17 °C. The values of the intrinsic rate of increase (rm, 0.108--0.200), net reproduction rate (R0, 18.71--36.47) and the mean generation time (T, 14.68--29.73) significantly differed among the five temperatures. The highest net reproduction rate (R0 = 36.47) was obtained at 25 °C. The results of this study indicated that A. andersoni has a high inherent potential for the control of the T. urticae at certain temperatures
2-(4-Methylphenyl)-5-[({[5-(4-methylphenyl)-1,3,4-thiadiazol-2-yl]sulfanyl}methyl)sulfanyl]-1,3,4-thiadiazole
In the title compound, C19H16N4S4, the molecules exhibit a butterfly conformation, where the thiadiazole and attached benzene rings in two wings are almost coplanar, with dihedral angles of 0.8 (3) and 0.9 (3)°, respectively, while the two thiadiazole rings form a dihedral angle of 46.3 (3)°
Time-resolved boson sampling with photons of different colors
Interference of multiple photons via a linear-optical network has profound
applications for quantum foundation, quantum metrology and quantum computation.
Particularly, a boson sampling experiment with a moderate number of photons
becomes intractable even for the most powerful classical computers, and will
lead to "quantum supremacy". Scaling up from small-scale experiments requires
highly indistinguishable single photons, which may be prohibited for many
physical systems. Here we experimentally demonstrate a time-resolved version of
boson sampling by using photons not overlapping in their frequency spectra from
three atomic-ensemble quantum memories. Time-resolved measurement enables us to
observe nonclassical multiphoton correlation landscapes. An average fidelity
over several interferometer configurations is measured to be 0.936(13), which
is mainly limited by high-order events. Symmetries in the landscapes are
identified to reflect symmetries of the optical network. Our work thus provides
a route towards quantum supremacy with distinguishable photons.Comment: 5 pages, 3 figures, 1 tabl
1-{1-[(2-Chlorothiazol-5-yl)methyl]-5-methyl-1H-1,2,3-triazol-4-yl}ethanone
In the title compound, C9H9ClN4OS, the two rings enclose a dihedral angle of 84.67 (11)°. Intermolecular C—H⋯O and C—H⋯N hydrogen bonds stabilize the crystal packing
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