Antigen Incorporation on Cryptosporidium parvum Oocyst Walls

Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (MerifluorÒ, Meridian Diagnostic Inc.) could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism

Characteristics of Chamaerops humilis L. var. humilis seed oil and study of the oxidative stability by blending with soybean oil

Herein we examine the characteristics of date seed oil extracted from Chamaerops humilis L. var. humilis seeds (HSO) cultivated in a gardening zone in Tunisia. Its physicochemical properties, fatty acid composition, and thermal and antioxidant properties were evaluated and compared with those of seed oil from another variety of Chamaerops humilis. The results showed that HSO possessed higher contents of oleic (44%) and linoleic (20%) acids than the other seed oil. The total tocopherol and tocotrienol content was 88 mg/100 g oil, where α-tocotrienol (64%) was the major isomer. The total phenolic (91 μg/g oil) and flavonoid contents (18 μg/g oil) of the HSO were determined, and its antioxidant capacities, measured in terms of ABTS and DPPH radical-scavenging capacities, were 210 µM TEAC/g DW and 4.3 mM TEAC/g DW, respectively. The oxidative stability index (OSI) of the oil was 16 h at 110 °C. Furthermore, the OSI of soybean oil was significantly enhanced upon blending with HSO. HSO exhibited higher thermal stability than the other oils and significantly different thermal behavior. The determination of fatty acid composition, physicochemical properties, bioactive content, oxidative stability, and thermal behavior of HSO demonstrated that this renewable resource can be used for edible purposes

Desarrollo de un kit rápido para el diagnóstico de la trichinellosis

Número de solicitud: 9502169La presente invención consiste en el desarrollo de un "kit" rápido capaz de detectar la presencia de anticuerpos específicos frente a Trichinella spiralis aplicados al diagnóstico de la trichinelosis, enfermedad parasitaria, del hombre, cerdo y otros animales empleados para el consumo cárnico. Este kit discrimina los casos positivos de los negativos, apareciendo una banda coloreada sobre un substrato inerte en los casos positivos. Como reactivos biológicos, puede emplearse sangre, suero, saliva u otros fluidos biológicos. Este kit puede aplicarse a la detección de anticuerpos de cualquier agente infeccioso, así como para la detección de sustancias capaces de comportarse como antígeno

Desarrollo de un "Kit" rápido para el diagnóstico de la Hidatidosis

Número de solicitud: 9600230La presente invención consiste en el desarrollo de un "kit" rápido de diagnóstico de la Hidatidosis, capaz de detectar la presencia de anticuerpos específicos frente a la parasitación por la larva de Echinococcus. El kit basado en una inmunocromatografía donde existe una banda reactiva sobre un sustrato inerte, capaz de retener los inmunocomplejos formados con los antígenos del parásito. Como reactivos biológicos, pueden emplearse sangre, suero, como fluidos biológicos. Este "kit" puede aplicarse a la detección de anticuerpos así como para la detección de sustancias capaces de comportarse como antígenes. Este "Kit" discrimina los casos positivos de los negativos apareciendo una banda coloreada sobre un sustrato inerte en los casos positivos

Antigen incorporation on Cryptosporidium parvum oocyst walls

Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocyst wall. Using immunoelectron microscopy we show that the antigen recognized by a monoclonal antibody used for diagnosis of cryptosporidiosis (Merifluor®, Meridian Diagnostic Inc.) could be found associated with vesicles in the space between the sporozoites and the oocysts wall, and incorporated to the outer oocyst wall by an unknown mechanism