17 research outputs found
Data (Kikuchi Yoshitomo)
Fitness parameters of symbiont infected (Bu+) and uninfected (Bu-) insects. Sheets: survival rate, male's fitness parameters, female's fitness parameters, time to reproduction, egg numbers, and symbiont titer
An innovative strategy for control of the chestnut weevil Curculio elephas (Coleoptera: Curculionidae) using Metarhizium brunneum
Relative rate tests of 16S rRNA gene sequences of the gut symbionts of pentatomid stinkbugs in comparison with allied free-living bacteria. (DOCX 163Â kb
Additional file 1: of Recurrent evolution of gut symbiotic bacteria in pentatomid stinkbugs
Stinkbug samples used in this study. (XLSX 18Â kb
Additional file 5: of Recurrent evolution of gut symbiotic bacteria in pentatomid stinkbugs
Bacterial taxa and their relevant parameters analyzed in Fig. 2. (DOCX 136 kb
Tago_Burks_MLtree_file
Maximum Likelihood tree of the Burkholderia spp. based on 16S rRNA gene sequences using MEGA 6
Tago_Burks_alignment_file
16S rRNA gene sequences of the Burkholderia spp. were aligned using MEGA 6
Tago_Oxygen_consumption_data
Tago_Oxygen_consumption_dat
DataSheet1_Symbiont coordinates stem cell proliferation, apoptosis, and morphogenesis of gut symbiotic organ in the stinkbug-Caballeronia symbiosis.pdf
The bean bug Riptortus pedestris obtains a specific bacterial symbiont, Caballeronia insecticola (Burkholderia insecticola), from the environmental soil and harbors it in the posterior midgut region that is composed of hundreds of crypts. While newly hatched aposymbiotic insects possess primordial midgut crypts with little or no lumen, colonization of C. insecticola triggers swift development of the symbiotic organ, forming enlarged and opened crypts, and the symbiont subsequently fills the luminal cavities of those mature crypts. The cellular processes of crypt development triggered by C. insecticola colonization are poorly understood. Here we identified a fundamental mechanism of the symbiont-mediated midgut development by investigating cell cycles of intestinal epithelial cells. Intestinal stem cells of the bean bug are located and proliferate at the crypt base. Differentiated enterocytes migrate upward along the epithelial cell layer of the crypt as the midgut develops, induction of apoptosis in enterocytes primarily occurred on the tip side of the crypts, and apoptotic cells then eventually were shed from the crypts into the hemolymph. The proliferation rate of the stem cells at the base of the crypts was low while a high apoptotic rate was observed at the crypt tip in aposymbiotic insects, resulting in undeveloped short crypts. On the contrary, the gut-colonizing C. insecticola promoted the proliferation of the stem cells at the base of crypts and simultaneously inhibited apoptosis at the tip of crypts, resulting in a net growth of the crypts and the generation of a crypt lumen that becomes colonized by the bacterial symbiont. These results demonstrated that the Caballeronia symbiont colonization induces the development of the midgut crypts via finely regulating the enterocyte cell cycles, enabling it to stably and abundantly colonize the generated spacious crypts of the bean bug host.</p
Tago_Community_pattern_data
Tago_Community_pattern_dat
Effects of Symbiont Elimination on Adult Body Size and Phenotype of the Plataspid Stinkbugs
<p>(A) Adult females of M. punctatissima (left) and M. cribraria (right) emerged from the control egg masses with capsules (top) and those from the treated egg masses without capsules (bottom). (B) Thorax width of M. punctatissima and (C) thorax width of <i>M. cribraria.</i> Means and standard deviations are shown. Open columns, with capsules; filled columns, without capsules. Sample sizes are labeled on the columns. Asterisks indicate statistically significant differences (median test; *** <i>p</i> < 0.001 after Bonferroni correction).</p