STRUCTURAL AND FUNCTIONAL STUDIES OF NONSTRUCTURAL PROTEIN 5 (NS5) FROM DENGUE VIRUS

Ph.DDOCTOR OF PHILOSOPH

Robust Multimodal Failure Detection for Microservice Systems

Proactive failure detection of instances is vitally essential to microservice systems because an instance failure can propagate to the whole system and degrade the system's performance. Over the years, many single-modal (i.e., metrics, logs, or traces) data-based nomaly detection methods have been proposed. However, they tend to miss a large number of failures and generate numerous false alarms because they ignore the correlation of multimodal data. In this work, we propose AnoFusion, an unsupervised failure detection approach, to proactively detect instance failures through multimodal data for microservice systems. It applies a Graph Transformer Network (GTN) to learn the correlation of the heterogeneous multimodal data and integrates a Graph Attention Network (GAT) with Gated Recurrent Unit (GRU) to address the challenges introduced by dynamically changing multimodal data. We evaluate the performance of AnoFusion through two datasets, demonstrating that it achieves the F1-score of 0.857 and 0.922, respectively, outperforming the state-of-the-art failure detection approaches

The Study on Newly Developed McAb NJ001 Specific to Non-Small Cell Lung Cancer and Its Biological Characteristics

Monoclonal antibody (McAb) is the key tool for cancer immunodiagnosis and immunotherapy. McAb-based immunotherapy that targets tumor antigens has had great achivement. In this study, a cell clone which kept secreting high-titer IgG1-type McAb named NJ001 against human non-small cell lung cancer (NSCLC) cells was obtained. The titer of purified NJ001 was 2×106. The antigen named SP70 of NSCLC specifically identified by NJ001 was proved to be a protein with the relative molecular mass (Mr) of 70 kDa. The results of immunohistochemical staining indicated that NJ001 could positively react to NSCLC, but weak positively or negatively react to human small-cell lung cancer (SCLC), pulmonary pseudotumor and other epithelial tumors. In soft agar assay, the colony formation efficiency in NJ001 groups decreased in a dose-dependent manner. For the concentration of 100 µg/ml, 200 µg/ml and 400 µg/ml, the inhibition ratio of colony formation was 23.4%, 62.5% and 100% respectively. Meanwhile, NJ001 caused significant reduction in tumor volume and tumor weight compared to control mice in lung cancer xenograft model. The tumor growth inhibition ratio in 200 µg, 400 µg and 800 µg NJ001 groups was 10.44%, 37.29% and 44.04%, respectively. NJ001 also led to cytomorphological changes and induced the apoptosis of human lung adenocarcinoma cell line SPC-A1 significantly. The newly developed NJ001 selectively reacted to NSCLC and exhibited anti-tumor activity both in vitro and in vivo. NJ001 is of great value concerning immunodiagnostics and immunotherapy for NSCLC and holds promise for further research regarding the mechanism underlying tumor progression of NSCLC