An extended stellar halo discovered in the Fornax dwarf spheroidal using Gaia EDR3

We have studied the extent of the Red Giant Branch stellar population in the Fornax dwarf spheroidal galaxy using the spatially extended and homogeneous data set from Gaia EDR3. Our preselection of stars belonging to Fornax is based on their proper motions, parallaxes and color-magnitude diagram. The latter criteria provide a Fornax star sample, which we further restrict by color and magnitude to eliminate contaminations due to either Milky Way stars or QSOs. The precision of the data has been sufficient to reach extremely small contaminations (0.02 to 0.3%), allowing us to reach to a background level 12 magnitudes deeper than the central surface brightness of Fornax. We discover a break in the density profile, which reveals the presence of an additional component that extents 2.1 degree in radius, i.e. 5.4 kpc, and almost seven times the half-light radius of Fornax. The extended new component represents 10% of the stellar mass of Fornax, and behaves like an extended halo. The absence of tidally elongated features at such an unprecedented depth (equivalent to $37.94\pm0.16$ mag ${\rm arcsec}^{-2}$ in V-band) rules out a possible role of tidal stripping. We suggest instead that Fornax is likely at first infall, and has lost its gas very recently, which consequently leads to a lack of gravity implying that residual stars have spherically expanded to form the newly discovered stellar halo of Fornax.Comment: 15 pages, 10 figures, 4 tables, MNRAS, in press, version based on proof

Revisiting mass estimates of the Milky Way

We use the rotation curve from Gaia data release (DR) 3 to estimate the mass of the Milky Way. We consider an Einasto density profile to model the dark matter component. We extrapolate and obtain a dynamical mass $M=2.75^{+3.11}_{-0.48}\times 10^{11} M_\odot$ at $112$ kpc. This lower-mass Milky Way is consistent with the significant declining rotation curve, and can provide new insights into our Galaxy and halo inhabitants.Comment: 4 pages, 2 figures, Accepted for publication in proceedings of IAU Symposium 379: Dynamical Masses of Local Group Galaxies, Potsdam, March 20-24, 202

Detection of the Keplerian decline in the Milky Way rotation curve

Our position inside the Galactic disc had prevented us from establishing an accurate rotation curve, until the advent of Gaia, whose third data release (Gaia DR3) made it possible to specify it up to twice the optical radius. We aim to establish a new rotation curve of the Galaxy from the Gaia DR3, by drastically reducing uncertainties and systematics, and with the goal to provide a new estimate of the mass of the Galaxy. We have compared different estimates, established a robust assessment of the systematic uncertainties, and addressed differences in methodologies, particularly regarding distance estimates. This results in a sharply decreasing rotation curve for the Milky Way, the decrease in velocity between 19.5 and 26.5 kpc is approximately 30 km s$^{-1}$. We have identified, for the first time, a Keplerian decline of the rotation curve, starting at $\sim$ 19 kpc and up to $\sim$ 26.5 kpc from the Galaxy center, while a flat rotation curve is rejected with a significance of 3$\sigma$. The total mass is revised downwards to $2.06^{+0.24}_{-0.13}\times 10^{11}\ M_{\odot}$, in agreement with an absence of significant mass increase at radii larger than 19 kpc. The upper limit of the total mass was evaluated by considering the upper values of velocity measurements, which leads to a strict, unsurpassable, limit of $5.4\times 10^{11}\ M_{\odot}$.Comment: 13 Pages, 13 Figures, accepted by Astronomy and Astrophysics (21st August 2023

Impacts of regreening on soil microbial community and its assembly process in open-pit mining area of the Loess Plateau

Vegetation restoration is an important indicator of ecosystem health in a mining area. Understanding the impact of vegetation restoration on the characteristics and assembly process of soil microbial community is very important to explore the resilience and self-sustaining mechanism of the restored ecosystem in a mining area. Therefore, this study uses MiSeq high-throughput sequencing and zero model to detect the composition of soil microbial communities, the characteristics of molecular ecological network, the key flora and its assembly mechanism in the shrubs (BL), coniferous forests (CF), broad-leaved forests (BF), mixed forests (MF), that have been reclaimed for 18 years in Antaibao open-pit mining dump, and the control plots (CK, undisturbed surrounding poplar forests that have continued to grow for more than 30 years) in Pingshuo, Loess Plateau. The results show that: ① The effects of different vegetation restoration types on the bacterial community α-diversity are significant (P < 0.05). Compared with CK, the Sobs and Shannon index of MF and CF have increased by 35.29%, 3.50% and 25.18%, 1.05%, respectively, whereas there is no significant difference in the α-diversity of fungal community among different vegetation restoration types. ② Actinobacteria, Chloroflexi, Proteobacteria and Acidobacteria dominate in the bacterial community, and the first two dominant phylum are significantly higher than CK, while the latter two are opposite (P < 0.05). Ascomycota and Basidiomycota are the dominant fungi in the fungal community. The former is significantly higher than CK, while the latter is opposite (P < 0.05). ③ The stochastic process dominates the construction process of the soil bacterial community. In addition to the MF soil fungal community dominated by deterministic process, other fungal communities are also dominated by a stochastic process. However, no matter which type of vegetation is restored, the dominant role of the randomness process on the assembly of the bacterial community is much higher than that of the fungal community. Moreover, Proteobacteria and Acidobacteria are key taxa of the bacterial network, while Mortierellales, Thelebolales, Chaetothyriales, and Hypocreales are the key taxa of the fungal network. Vegetation restoration affects microbial community diversity, BL, CF, and MF increase the stability of the bacterial network, and MF makes fungal network more complex. The soil bacterial assembly process is dominated by stochastic processes, except for MF, and the fungal assembly process is also dominated by stochastic processes

Biosynthesis of depsipeptides with a 3-hydroxybenzoate moiety and selective anticancer activities involves a chorismatase.

Neoantimycins are anticancer compounds of 15-membered ring antimycin-type depsipeptides. They are biosynthesized by a hybrid multimodular protein complex of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS), typically from the starting precursor 3-formamidosalicylate. Examining fermentation extracts of Streptomyces conglobatus, here we discovered four new neoantimycin analogs, unantimycins B-E, in which 3-formamidosalicylates are replaced by an unusual 3-hydroxybenzoate (3-HBA) moiety. Unantimycins B-E exhibited levels of anticancer activities similar to those of the chemotherapeutic drug cisplatin in human lung cancer, colorectal cancer, and melanoma cells. Notably, they mostly displayed no significant toxicity toward noncancerous cells, unlike the serious toxicities generally reported for antimycin-type natural products. Using site-directed mutagenesis and heterologous expression, we found that unantimycin productions are correlated with the activity of a chorismatase homolog, the nat-hyg5 gene, from a type I PKS gene cluster. Biochemical analysis confirmed that the catalytic activity of Nat-hyg5 generates 3-HBA from chorismate. Finally, we achieved selective production of unantimycins B and C by engineering a chassis host. On the basis of these findings, we propose that unantimycin biosynthesis is directed by the neoantimycin-producing NRPS-PKS complex and initiated with the starter unit of 3-HBA. The elucidation of the biosynthetic unantimycin pathway reported here paves the way to improve the yield of these compounds for evaluation in oncotherapeutic applications

Identification of human neutralizing antibodies against MERS-CoV and their role in virus adaptive evolution

The newly emerging Middle East Respiratory Syndrome coronavirus (MERS-CoV) causes a Severe Acute Respiratory Syndrome-like disease with ∼43% mortality. Given the recent detection of virus in dromedary camels, zoonotic transfer of MERS-CoV to humans is suspected. In addition, little is known about the role of human neutralizing Ab (nAb) pressure as a driving force in MERS-CoV adaptive evolution. Here, we used a well-characterized nonimmune human Ab-phage library and a panning strategy with proteoliposomes and cells to identify seven human nAbs against the receptor-binding domain (RBD) of the MERS-CoV Spike protein. These nAbs bind to three different epitopes in the RBD and human dipeptidyl peptidase 4 (hDPP4) interface with subnanomolar/nanomolar binding affinities and block the binding of MERS-CoV Spike protein with its hDPP4 receptor. Escape mutant assays identified five amino acid residues that are critical for neutralization escape. Despite the close proximity of the three epitopes on the RBD interface, escape from one epitope did not have a major impact on neutralization with Abs directed to a different epitope. Importantly, the majority of escape mutations had negative impacts on hDPP4 receptor binding and viral fitness. To our knowledge, these results provide the first report on human nAbs against MERS-CoV that may contribute to MERS-CoV clearance and evolution. Moreover, in the absence of a licensed vaccine or antiviral for MERS, this panel of nAbs offers the possibility of developing human mAb-based immunotherapy, especially for health-care workers

Self-Confirmation and Ascertainment of the Candidate Genomic Regions of Complex Trait Loci - A None-Experimental Solution.

Over the past half century, thousands of quantitative trait loci (QTL) have been identified by using animal models and plant populations. However, the none-reliability and imprecision of the genomic regions of these loci have remained the major hurdle for the identification of the causal genes for the correspondent traits. We used a none-experimental strategy of strain number reduction for testing accuracy and ascertainment of the candidate region for QTL. We tested the strategy in over 400 analyses with data from 47 studies. These studies include: 1) studies with recombinant inbred (RI) strains of mice. We first tested two previously mapped QTL with well-defined genomic regions; We then tested additional four studies with known QTL regions; and finally we examined the reliability of QTL in 38 sets of data which are produced from relatively large numbers of RI strains, derived from C57BL/6J (B6) X DBA/2J (D2), known as BXD RI mouse strains; 2) studies with RI strains of rats and plants; and 3) studies using F2 populations in mice, rats and plants. In these cases, our method identified the reliability of mapped QTL and localized the candidate genes into the defined genomic regions. Our data also suggests that LRS score produced by permutation tests does not necessarily confirm the reliability of the QTL. Number of strains are not the reliable indicators for the accuracy of QTL either. Our strategy determines the reliability and accuracy of the genomic region of a QTL without any additional experimental study such as congenic breeding

Inhibiting function of human fetal dermal mesenchymal stem cells on bioactivities of keloid fibroblasts

Abstract Background Keloid is one kind of benign skin disease caused by hyperplasia of fibroblasts and collagen fibrils. It is refractory due to the lack of an effective treatment at present, which puts pressure on seeking a new therapeutic regimen. Mesenchymal stem cells (MSCs) from fetal skin are considered to play a crucial role in scarless healing. Nevertheless, the efficacy of them in keloid disorders remains poorly understood. Methods Keloid fibroblasts (KFs), human adult dermal fibroblasts (ADFs), and human fetal dermal mesenchymal stem cells (FDMSCs) were isolated to single cells and cultured in Dulbecco’s modified Eagle’s medium (DMEM). ADFs and FDMSCs were used to generate ADF-conditioned medium (A-CM) and FDMSC-conditioned medium (F-CM). The effects of A-CM and F-CM on KFs were tested using MTT assay, BrdU assay, TUNEL assay, quantitative polymerase chain reaction, Western blot, and annexin V-FITC/PI binding assay,. Results FDMSCs inhibited the bioactivity of KFs, downregulated the expression of the antiapoptotic protein BCL-2, and upregulated the expression of the proapoptotic protein BAX of KFs by secreting some soluble substances, thus accelerating the apoptosis of KFs. Conclusion F-CM induces apoptosis of KFs, providing a novel treatment strategy for keloid disorders