Investigation of Some Immunological Parameters in Rainbow Trout (Oncorhynchus mykiss) Injected Propolis

In this study, effects of propolis on immunological parameters of rainbow trout were investigated. For this purpose, propolis was intraperitoneally injected to fish 4 times at doses of 2.5, 5 and 10 mg / kg fish weight. Blood samples on 3, 9, 15 and 21 days were collected from fish of the control and experimental groups and analysed to determine oxidative radical production [nitroblue tetrazolium (NBT) activity], total plasma protein and total immunoglobulin levels. At the end of the experiment, there was a statistically significant increase in oxidative radical production, total protein and immunoglobulin levels of propolis treated groups when compared to the control group

Propolisin Genel Özellikleri ve Balıklarda Kullanımı

Propolis, bitkilerin tomurcuk ve yaprak kısımlarından toplanan maddelerden bal arıları tarafından üretilen doğal reçineli bir karışımdır. Propolis antikanser, antiinflamator, antibiyotik, antioksidatif, antibakteriyel, antiviral, antifungal, anestetik, immunostimulan ve sitotoksik etkilere sahiptir. Bu derlemede doğal bir ürün olan propolisin fiziksel, kimyasal ve biyolojik özellikleri, bitkisel kaynakları ile balıklarda kullanımı ve uygulama şekiller hakkındaki bilgilerin bir araya getirilmesi amaçlanmıştır

Effects of dietary propolis on the number and size of pleopadal egg, oxidative stress and antioxidant status of freshwater crayfish (Astacus leptodactylus Eschscholtz)

Four different crayfish diets; control, El, E2 and E3, respectively containing 0, 1, 2 and 4% propolis, were tested to determine the effects of dietary propolis on the number and size of pleopadal egg, and malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities in the freshwater crayfish (Astacus leptodactylus). The crayfish were kept at 9.6 +/- 5.3 degrees C water temperature and fed three times daily during a six month period The pleopodal egg number (from 7 to 9) produced per gram of the bpdy weight and total pleopodal egg number (from 201 to 263) significantly increased (P < 0.05) with the dietary propolis supplementation. However, an increase in the dietary propolis led to a significant decrease (P < 0.05) in the pleopodal egg size (from 3.22 mm to 2.76 mm). MDA level significantly (P < 0.05) decreased in the hepatopancreas (from 4.78 to 3.04 nmol/g protein) and ovarium (from 3.52 to 1.98 nmol/g protein) of the crayfish fed with the increased dietary propolis level. On the other hand, an increase in the dietary propolis led to a significant increase (P < 0.05) in SOD activities in hepatopancreas (from 21.8 to 41.1 U/g protein) and ovarium (from 16.8 to 26.8 U/g protein). However, CAT activities significantly decreased (P < 0.05) in the hepatopancreas (from 23.8 to 18.9 nmol/g protein) and ovarium (from 21.8 to 17.5 nmol/g protein) of the crayfish fed with the increased dietary propolis level. Similarly, an increase in the dietary propolis caused a significant decrease (P < 0.05) in GSH-Px activities in the hepatopancreas (from 21.8 to 41.1 U/g protein) and ovarium (from 16.8 to 26.8 U/g protein) with the formation of the pleopodal egg. The dietary propolis improves reproductive efficiency in the crayfish and decreases the oxidative stress under controlled hatchery conditions