Implementation of fatigue model for unidirectional laminate based on finite element analysis : theory and practice

The aim of this study is to deal with the simulation of intra-laminar fatigue damage in unidirectional composite under multi-axial and variable amplitude loadings. The variable amplitude and multi-axial loading is accounted for by using the damage hysteresis operator based on Brokate method [6]. The proposed damage model for fatigue is based on stiffness degradation laws from Van Paepegem combined with the 'damage' cycle jump approach extended to deal with unidirectional carbon fibres. The parameter identification method is here presented and parameter sensitivities are discussed. The initial static damage of the material is accounted for by using the LadevSze damage model and the permanent shear strain accumulation based on Van Paepegem's formulation. This approach is implemented into commercial software (Siemens PLM). The validation case is run on a bending test coupon (with arbitrary stacking sequence and load level) in order to minimise the risk of inter-laminar damages. This intra-laminar fatigue damage model combined efficient methods with a low number of tests to identify the parameters of the stiffness degradation law, this overall procedure for fatigue life prediction is demonstrated to be cost efficient at industrial level. This work concludes on the next challenges to be addressed (validation tests, multiple-loadings validation, failure criteria, inter-laminar damages...)

A progessive damage fatigue model for unidirectional laminated composites based on finite element analysis: theory and practice

The simulation of the fatigue damage of laminated composites under multi-axial and variable amplitude loadings has to deal with several new challenges and several methods of damage modelling. In this paper we present how to account for the complex loading by using the damage hysteresis operator approach for fatigue. It is applied to a fatigue model for intra-laminar damage based on stiffness degradation laws from Van Paepegem and has been extended to deal with unidirectional carbon fibres. The parameter identification method is presented here and parameter sensitivities are discussed. The initial static damage of the material is accounted for by using the Ladevèze damage model and the permanent shear strain accumulation based on Van Paepegem’s formulation. This approach has been implemented into commercial software. The intra-laminar fatigue damage model combines efficient methods with a low number of tests to identify the parameters of the stiffness degradation law, this overall procedure for fatigue life prediction is demonstrated to be cost efficient at industrial level

A progessive damage fatigue model for unidirectional laminated composites based on finite element analysis: theory and practice

The simulation of the fatigue damage of laminated composites under multi-axial and variable amplitude loadings has to deal with several new challenges and several methods of damage modelling. In this paper we present how to account for the complex loading by using the damage hysteresis operator approach for fatigue. It is applied to a fatigue model for intra-laminar damage based on stiffness degradation laws from Van Paepegem and has been extended to deal with unidirectional carbon fibres. The parameter identification method is presented here and parameter sensitivities are discussed. The initial static damage of the material is accounted for by using the Ladevèze damage model and the permanent shear strain accumulation based on Van Paepegem’s formulation. This approach has been implemented into commercial software. The intra-laminar fatigue damage model combines efficient methods with a low number of tests to identify the parameters of the stiffness degradation law, this overall procedure for fatigue life prediction is demonstrated to be cost efficient at industrial level

Meltrin β/ADAM19 Interacting with EphA4 in Developing Neural Cells Participates in Formation of the Neuromuscular Junction

BACKGROUND: Development of the neuromuscular junction (NMJ) is initiated by the formation of postsynaptic specializations in the central zones of muscles, followed by the arrival of motor nerve terminals opposite the postsynaptic regions. The post- and presynaptic components are then stabilized and modified to form mature synapses. Roles of ADAM (A Disintegrin And Metalloprotease) family proteins in the formation of the NMJ have not been reported previously. PRINCIPAL FINDINGS: We report here that Meltrin beta, ADAM19, participates in the formation of the NMJ. The zone of acetylcholine receptor alpha mRNA distribution was broader and excess sprouting of motor nerve terminals was more prominent in meltrin beta-deficient than in wild-type embryonic diaphragms. A microarray analysis revealed that the preferential distribution of ephrin-A5 mRNA in the synaptic region of muscles was aberrant in the meltrin beta-deficient muscles. Excess sprouting of motor nerve terminals was also found in ephrin-A5 knockout mice, which lead us to investigate a possible link between Meltrin beta and ephrin-A5-Eph signaling in the development of the NMJ. Meltrin beta and EphA4 interacted with each other in developing motor neurons, and both of these proteins localized in the NMJ. Coexpression of Meltrin beta and EphA4 strongly blocked vesicular internalization of ephrin-A5-EphA4 complexes without requiring the protease activity of Meltrin beta, suggesting a regulatory role of Meltrin beta in ephrin-A5-Eph signaling. CONCLUSION: Meltrin beta plays a regulatory role in formation of the NMJ. The endocytosis of ephrin-Eph complexes is required for efficient contact-dependent repulsion between ephrin and Eph. We propose that Meltrin beta stabilizes the interaction between ephrin-A5 and EphA4 by regulating endocytosis of the ephrinA5-EphA complex negatively, which would contribute to the fine-tuning of the NMJ during development

Differential Neuregulin 1 Cleavage in the Prefrontal Cortex and Hippocampus in Schizophrenia and Bipolar Disorder: Preliminary Findings

Neuregulin 1 (NRG1) is a key candidate susceptibility gene for both schizophrenia (SCZ) and bipolar disorder (BPD). The function of the NRG1 transmembrane proteins is regulated by cleavage. Alteration of membrane bound-NRG1 cleavage has been previously shown to be associated with behavioral impairments in mouse models lacking expression of NRG1-cleavage enzymes such as BACE1 and gamma secretase. We sought to determine whether alterations in NRG1 cleavage and associated enzymes occur in patients with SCZ and BPD.Using human postmortem brain, we evaluated protein expression of NRG1 cleavage products and enzymes that cleave at the external (BACE1, ADAM17, ADAM19) and internal (PS1-gamma secretase) sides of the cell membrane. We used three different cohorts (Controls, SCZ and BPD) and two distinct brain regions: BA9-prefrontal cortex (Controls (n = 6), SCZ (n = 6) and BPD (n = 6)) and hippocampus (Controls (n = 5), SCZ (n = 6) and BPD (n = 6)). In BA9, the ratio of the NRG1 N-terminal fragment relative to full length was significantly upregulated in the SCZ cohort (Bonferroni test, p = 0.011). ADAM17 was negatively correlated with full length NRG1 levels in the SCZ cohort (r = -0.926, p = 0.008). In the hippocampus we found significantly lower levels of a soluble 50 kDa NRG1 fragment in the two affected groups compared the control cohort (Bonferroni test, p = 0.0018). We also examined the relationship of specific symptomatology criteria with measures of NRG1 cleavage using the Bipolar Inventory of Signs and Symptoms Scale (BISS) and the Montgomery Åsberg Depression Rating Scale (MADRS). Our results showed a positive correlation between ADAM19 and psychosis (r = 0.595 p = 0.019); PS1 and mania (r = 0.535, p = 0.040); PS1 and depression (r = 0.567, p = 0.027) in BA9, and BACE1 with anxiety (r = 0.608, p = 0.03) in the hippocampus.Our preliminary findings suggest region-specific alterations in NRG1 cleavage in SCZ and BPD patients. These changes may be associated with specific symptoms in these psychiatric disorders

Relativistic double-zeta, triple-zeta, and quadruple-zeta basis sets for the lanthanides La–Lu

Relativistic basis sets of double-zeta, triple-zeta, and quadruple-zeta quality have been optimized for the lanthanide elements La-Lu. The basis sets include SCF exponents for the occupied spinors and for the 6p shell, exponents of correlating functions for the valence shells (4f, 5d and 6s) and the outer core shells (4d, 5s and 5p), and diffuse functions, including functions for dipole polarization of the 4f shell. A finite nuclear size was used in all optimizations. The basis sets are illustrated by calculations on YbF. Prescriptions are given for constructing contracted basis sets. The basis sets are available as an internet archive and from the Dirac program web site, http://dirac. chem. sdu. dk. © 2010 The Author(s)

Transition and Stability of Copolymer Adsorption Morphologies on the Surface of Carbon Nanotubes and Implications on Their Dispersion

In this study, the adsorption morphologies as well as stability and transitions of a commercial dispersant copolymer (BYK 9076) on the surface of multiwalled carbon nanotubes (MWCNTs) were studied using Fourier transform infrared and UV-vis spectroscopy, dynamic light scattering, and electron microscopy techniques. The results show that the dispersion of carbon nanotubes in ethanol does not increase continuously with increasing copolymer/CNT ratio, which is correlated with the adsorption morphologies of the copolymer on the CNT surface. At a ratio of copolymer/CNT below 0.5, the morphology is random, shifting to a hemimicelle structure at a ratio from 0.5 to 1.0 while at ratios above 1.0, a cylindrical pattern is seen. The hemimicelle morphology is able to prevent the agglomeration of CNTs when the CNT concentration increases to 8.7 mg/mL, while cylindrical morphology is more efficient and stable to provide dispersion of CNTs at higher concentrations of CNTs