X-ray Study Of Herbaspirilum Seropedicae Glnb Protein Adsorbed On Silicon

GlnB-Hs protein is a globular member of PII-like family involved in the nitrogen control fixation in diazothoph organisms like H. seropedicae. In this work, GlnB-Hs is deposited by spin-coating on Si (in) and its self-assembling is investigated by AFM (atomic force microscopy) and X-ray at grazing incidence angles. GlnB-Hs forms circular 100 nm to 150 nm wide face-up donut shaped protein aggregates on silicon. Reflectivity and diffraction profiles from the protein deposits on Si(111) and Si(100), suggest ordered domains that are stable under the X-ray beam and have d-spacing compatible with protein dimensions already determined by classical protein crystallography. Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA.245-246140146Gray, J., Curr. Opi. in Struct. Biology, 1004 (14), p. 110Sleytr, U., Gyorvary, E., (2003) Progr. in Org. Coating, 47, p. 279Pechkova, E., Nicolini, C., (2004) Trends in Bio, 22, p. 117Pechkova, E., Nicolini, C., (2001) J. of Crystal growth, 231, p. 599Giegé, R., Drenth, J., Ducruix, A., McPherson, A., Saenger, W., (1995) Prog. Cryst. Growth and charact, 30, p. 237Jap, B.K., Zulauf, M., Scheybani, T., Hefti, A., Baumeister, W., Aebi, U., Engel, A., (1992) Ultramicroscopy, 46, p. 45Als-Nielsen, J., Jacquemain, D., Kjaer, K., Leveiller, F., Lahav, M., Leiserowitz, L., (1994) Physics reports, 246, p. 251Samant, M.G., Brown, C.A., Gordon II, J.C., (1991) Langmuir, 7, p. 437Seul, M., Eisenberger, P., McCOnnell, H.M., (1983) PNAS, 80, p. 5795Fenter, P., Eisenberger, P., Li, J., Camillone, N., Bernasek, S., Scoles, G., Ramanarayanan, T.A., Liang, K.S., (1991) Langmuir, 7, p. 2013Salditt, T., Mennicke, U., (2002) Longmuir, 18, p. 8172Verclas, S.A.W., Howes, P.B., Kjaer, K., Wurlitzer, A., Weygand, M., Buldt, G., Dencher, N.A., Lösche, M., (1999) J.M.B, 287, p. 837Lawrence, C.J., Zhou, W., (1991) J. of Non-Newtonian fluid Mech, 39, p. 137Ninfa, A.J., Atkinson, M.R., (2000) Trends Microbiol, 8, p. 172Baldani, V.L.D., Baldani, J.I., Olivares, F., Dobereiner, J., (1992) Symbiosis, 13, p. 65Baldani, J.I., Baldani, V.L.D., Seldin, L., Dobereiner, J., (1986) Int. J. Syst. Bacteriol, 36, p. 86Pimentel, J.P., Olivares, F., Pitard, R.M., Urquiaga, S., Akiba, F., Dobereiner, J., (1991) Plant Soil, 137, p. 61Weber, O.B., Baldani, V.L.D., Teixeira, K.R.S., Kirchhof, C., Baldani, J.I., Doberreiner, J., (1999) Plont Soil, 210, p. 103Benelli, E.M., de Souza, E.M., Funayama, S., Rigo, L.U., Pedrosa, F.O., (1997) J. Bacteriol, 179, p. 4623de Souza, E.M., Pedrosa, F.O., Drummond, M.H., Rigo, L.U., Yates, M.G., (1999) J. Bacteriol, 181, p. 681Benelli, E.M., Buck, M., de Souza, E.M., Yates, M.G., Pedrosa, F.O., (2001) Can. J. Microbiol, 47, p. 309Benelli, E.M., Buck, M., Polikarpov, I., de Souza, E.M., Cruz, L.M., Pedrosa, F.O., (2002) Eur. J. Biochem, 269, p. 3296Hirose, M., Yasaka, T., Takakura, M., Miyazaki, S., (1991) Solid State Technology, p. 43Windt, D.L., (1998) Computers in physics, 12, p. 360Lubambo, A.F., Benelli, E.M., Klein, J., Schreiner, W., de Camargo, P.C., (2006) Cell Bio. and Biophysics, 44, p. 503Guinier, A., (1963) X-Ray Diffraction, p. 121. , Freeman, San FranciscoPechkova, E., Fiodoro, S., Fontani, D., Nicolini, C., (2005) Acta Crysta. D, 61, p. 809Adachi, E., Nagayama, K., (1997) Adv. Biophys, 34, p. 81Kralchevsky, P., Denkov, N.D., (2001) Cur. Opi. In Colloid & Inter. Sci, 6, p. 383Ravelli, R., McSweeneney, S.M., (2000) Structure, 8, p. 315Nave, C., Hill, M.A., (2005) J. Sync. Rad, 12, p. 261Ashley, J.C., (1980) IEEE Trans. on Nucl. Scien, 27, p. 1454M. J. Berger, J. S. Coursey, M. A. Zucker. V 1.2.2., NIST, Gaithersburg, MD, USA 2000Hovington, P., Drouin, D., Gauvin, R., (1997) Scanning, 19, p. 1Murray, J.W., Garman, E.F., Ravelli, R.B.G., (2004) J. of Appl. Cryst, 37, p. 51

Hyaluronic acid in Pluronic F 127 F 108 hydrogels for postoperative pain in arthroplasties Influence on physico chemical properties and structural requirements for sustained drug release

Submitted by Marco Antonio Oliveira da Silva ([email protected]) on 2018-07-19T12:16:29Z No. of bitstreams: 1 24763.pdf: 2948426 bytes, checksum: a466feea2de68fad683771c10424f0c7 (MD5)Made available in DSpace on 2018-07-19T12:16:29Z (GMT). No. of bitstreams: 1 24763.pdf: 2948426 bytes, checksum: a466feea2de68fad683771c10424f0c7 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)In this study,we reported the hyaluronic acid (HA) on supramolecular structure of Pluronic F-127 (PLF-127) and/ or Pluronic F-108 (PLF-127) hydrogels, as well as their effects on release mechanisms, looking forward their application as lidocaine (LDC) drug-delivery systems in arthroplastic surgeries.We have studied the HA-micelle interaction using Dynamic Light Scattering (DLS), themicellization and sol-gel transition processes by Differential Scanning Calorimetry (DSC) and Rheology., of PL-based hydrogels and. The presence of HA provided the formation of larger micellar dimensions from ~26.0 to 42.4 nm. The incorporation of HA did not change the micellization temperatures and stabilized hydrogels rheological properties (G′ N G″), showing no interference on PLthermoreversible properties. Small-Angle-X-ray Scattering (SAXS) patterns revealed that HA incorporation effects were pronounced for PLF-127 and PLF-108 systems, showing transitions from lamellar to hexagonal phase organization (HA-PLF-127) and structural changes from cubic to gyroid and/or cubic to lamellar. The HA insertion effects were also observed on drug release profiles, since lower LDC release constants (Krel = 0.24–0.41 mM·h−1) were observed for HA-PLF-127, that presented a hexagonal phase organization. Furthermore, the HA-PL systems presented reduced in vitro cytotoxic effects, pointed out their tendency to selfassembly and possible application as drug delivery systems.CAPES: 00.889.834/0001-08FAPESP: 14/26200-9; 14/14457-5; 15/14763-1CNPq: 309207/2016-9; 402838/2016-