ペロブスカイト型酸化物触媒を用いたガス拡散型酸素電極に関する研究

第1章 緒論 第2章 ペロブスカイト型酸化物触媒による電気化学的酸素還元の機構 第3章 酸素還元電極における触媒の添加効果 第4章 ガス拡散型酸素電極の至適構造の検討 第5章 高表面積ペロブスカイト型酸化物触媒を用いたガス拡散型酸素還元電極 第6章 ガス拡散型酸素電極の2元機能特性と金属-空気電池への応用 第7章 本研究の総括Made available in DSpace on 2012-09-06T02:19:41Z (GMT). No. of bitstreams: 3 shimizu1.pdf: 11650186 bytes, checksum: 6e5f45fff5e4f1fb2832abe105da2a2b (MD5) shimizu2.pdf: 8467192 bytes, checksum: e1d290d6b3ded1bb265baf45656ed606 (MD5) shimizu3.pdf: 11797911 bytes, checksum: cd9f288486a96a6bf244f09fe1a5dfc8 (MD5) Previous issue date: 1992-11-1

Effects of five-minute internet-based cognitive behavioral therapy and simplified emotion-focused mindfulness on depressive symptoms: a randomized controlled trial

Simplified Emotion-focused Mindfulness Exercise (English translation, original in Japanese). (DOCX 37 kb

A list of fishes found on the oyster farming rafts by the underwater visual census in northern Hiroshima Bay, Seto Inland Sea, Japan

カキ筏には多様な付着生物が生育することが知られており，魚類にとっても寄り付きやすい環境であることが示唆されている。しかし，カキ筏に具体的にどのような魚類が出現するのかについての知見は乏しい。そこで，カキ筏が数多く係留されている広島湾北部の江田島沖合において，カキ筏に出現する魚類の潜水目視調査を2011年8月と10月に実施した。マガキの垂下された水深5-9mを筏に沿って約50m 遊泳しながら，水中構造内に存在する魚種と個体数を記録した。各月とも，マガキの成長した筏とマガキが垂下されて間もない筏について調査し，のべ400分の潜水観察により総計16科24種の魚類を確認した。そのほぼすべてが，沿岸岩礁域およびガラモ場にみられる魚種であった。クロダイ，アカオビシマハゼ，アミメハギ，カワハギの４種は，調査を実施した筏すべてにおいて出現が認められ，カキ筏環境への順応性の高さが伺われた。出現魚種数および出現個体数は，マガキの成長した筏が有意に高く，マガキの成長に伴い，より多様な魚が数多く寄り付きやすくなることが示唆された。The oyster farming rafts occupy 200ha of the water surface in Hiroshima Bay. Each floating raft (ca.11m wide x 26m long) hangs a total of 600 wires (ca. 10m depth) each attaching 40 oysters’ collectors, providing a complex underwater structure. Though the raft structure has been suggested to provide nursery habitats for fishes, information of fishes found around the oyster raft has been very limited hitherto. In order to obtain data of the species composition and the abundance of fishes occurring on the raft structure, we conducted visual census at off Eta-shima Island, northern Hiroshima Bay, during the daytime on August 5 and October 3, 2011. The collecters were dropped in 0-15m depth from floating rafts. Five observers swam for 52m along the outer edge of the raft in 5-9m depth, with recording fish species and individual numbers occurring within the structure (ca. 100min observation for each raft). The underwater survey was conducted for two types of the rafts in each survey month; a raft with well-grown oysters after overwintering (called the late raft) and one with oyster spats (called the early raft). A total of 24 fish species (16 families) were confirmed and almost of them (23 species) have ever been known as occurring on rocky reefs in Hiroshima Bay. Four fishes, a sparid Acanthopagrus schlegelii, a gobiid Tridentiger trigonocephalus, monacanthids Rudarius ercodes and Stephanolepis cirrhifer, commonly occurred in all four surveyed rafts, suggesting their high abilities in fitting for the environmental conditions of the oyster rafts. The late rafts maintained significantly higher values both in mean fish species numbers and in mean fish abundance than the early rafts, implying that the environmental conditions would become attractive for fishes as a nursery habitat in accordance with the growth of oysters mooring for longer periods.本研究は平成23年度広島大学地域連携推進研究事業の支援を受け，プロジェクト「魚類によるカキならびにアサリの食害防除に関する生物学的研究」の一環として実施したものである

潜水センサスを用いた瀬戸内海倉橋島における浅海魚類相 : 出現魚種の季節的消長 <原著論文>

瀬戸内海の倉橋島において，潜水センサスによる魚類相の周年調査を行った。本調査により，8目29科53種の魚類を確認した。これらは周年定住種16種，季節的定住種37種に分けられた。近年，瀬戸内海で報告されている暖海性魚類と思われる種は確認されなかった。月毎の出現魚種数は，9・10月の41種が最多であり，1・2月の18種が最少であった。冬期には，9・10月に認められた多くの季節的定住種はその姿を消し，周年定住種を中心とした魚類群集が形成された。本調査海域の冬期の最低水温は10℃であり，低水温が種数の変動に影響し，暖海性魚類の出現を制限する要因になっていると考えられた。We conducted a year-long underwater census to survey fish fauna on a reef off Kurahashi Island in the Seto Inland Sea. A total of 53 species (8 orders, 29 families) were recorded; 16 species were residents, 37 were seasonal residents. No tropical/warm-temperate fish were confirmed. The total number of fish species (41) peaked in September and October, but drastically decreased to just 18 species in January and February because of the disappearance of most seasonal residents. These seasonal residents appeared to be prevented from continuing residence in the central part of the Seto Inland Sea because of the low water temperature in winter.※誤植訂正のためPDF差し替え(2018年3月20日

Increased [¹⁸F]FMISO accumulation under hypoxia by multidrug-resistant protein 1 inhibitors

BACKGROUND: [¹⁸F]Fluoromisonidazole ([¹⁸F]FMISO) is a PET imaging probe widely used for the detection of hypoxia. We previously reported that [¹⁸F]FMISO is metabolized to the glutathione conjugate of the reduced form in hypoxic cells. In addition, we found that the [¹⁸F]FMISO uptake level varied depending on the cellular glutathione conjugation and excretion ability such as enzyme activity of glutathione-S-transferase and expression levels of multidrug resistance-associated protein 1 (MRP1, an efflux transporter), in addition to the cellular hypoxic state. In this study, we evaluated whether MRP1 activity affected [¹⁸F]FMISO PET imaging. METHODS: FaDu human pharyngeal squamous cell carcinoma cells were pretreated with MRP1 inhibitors (cyclosporine A, lapatinib, or MK-571) for 1 h, incubated with [¹⁸F]FMISO for 4 h under hypoxia, and their radioactivity was then measured. FaDu tumor-bearing mice were intravenously injected with [¹⁸F]FMISO, and PET/CT images were acquired at 4 h post-injection (1st PET scan). Two days later, the same mice were pretreated with MRP1 inhibitors (cyclosporine A, lapatinib, or MK-571) for 1 h, and PET/CT images were acquired (2nd PET scan). RESULTS: FaDu cells pretreated with MRP1 inhibitors exhibited significantly higher radioactivity than those without inhibitor treatment (cyclosporine A: 6.91 ± 0.27, lapatinib: 10.03 ± 0.47, MK-571: 10.15 ± 0.44%dose/mg protein, p < 0.01). In the in vivo PET study, the SUVmean ratio in tumors [calculated as after treatment (2nd PET scan)/before treatment of MRP1 inhibitors (1st PET scan)] of the mice treated with MRP1 inhibitors was significantly higher than those of control mice (cyclosporine A: 2.6 ± 0.7, lapatinib: 2.2 ± 0.7, MK-571: 2.2 ± 0.7, control: 1.2 ± 0.2, p < 0.05). CONCLUSION: In this study, we revealed that MRP1 inhibitors increase [¹⁸F]FMISO accumulation in hypoxic cells. This suggests that [¹⁸F]FMISO-PET imaging is affected by MRP1 inhibitors independent of the hypoxic state

ANGPTL4 Expression Is Increased in Epicardial Adipose Tissue of Patients with Coronary Artery Disease

Epicardial adipose tissue (EAT) is known to affect atherosclerosis and coronary artery disease (CAD) pathogenesis, persistently releasing pro-inflammatory adipokines that affect the myocardium and coronary arteries. Angiopoietin-like 4 (ANGPTL4) is a protein secreted from adipose tissue and plays a critical role in the progression of atherosclerosis. Here, the expression of ANGPTL4 in EAT was investigated in CAD subjects. Thirty-four consecutive patients (13 patients with significant CAD; 21 patients without CAD) undergoing elective open-heart surgery were recruited. EAT and pericardial fluid were obtained at the time of surgery. mRNA expression and ANGPTL4 and IL-1β levels were evaluated by qRT-PCR and ELISA. The expression of ANGPTL4 (p = 0.0180) and IL-1β (p < 0.0001) in EAT significantly increased in the CAD group compared to that in the non-CAD group and positively correlated (p = 0.004). Multiple regression analysis indicated that CAD is a contributing factor for ANGPTL4 expression in EAT. IL-1β level in the pericardial fluid was significantly increased in patients with CAD (p = 0.020). Moreover, the expression of ANGPTL4 (p = 0.004) and IL-1β (p < 0.001) in EAT was significantly increased in non-obese patients with CAD. In summary, ANGPTL4 expression in EAT was increased in CAD patients