Functional Multiplicity of an Insect Cytokine Family Assists Defense Against Environmental Stress

The widespread distribution of insects over many ecological niches owes much to evolution of multiple mechanisms to defend against environmental stress, especially because their ectothermic nature and small body size render them particularly susceptible to extremes in temperature and water availability. In this review, we will summarize the latest information describing a single, multifunctional cytokine family that is deployed by six orders of insect species to combat a diverse variety of environmental stresses. The originating member of this peptide family was identified in Mythimna (formerly called Pseudaletia) separata armyworm; the cytokine was named growth-blocking peptide (GBP), reflecting its actions in combating parasitic invasion. The peptide’s name has been retained, though the list of its regulatory activities has greatly expanded. All members of this family are small peptides, 19–25 amino acid residues, whose major source is fat body. They are now known to regulate embryonic morphogenesis, larval growth rates, feeding activities, immune responses, nutrition, and aging. In this review, we will describe recent developments in our understanding of the mechanisms of action of the GBP family, but we will also highlight remaining gaps in our knowledge

Updated absolute gravity rate of change associated with glacial isostatic adjustment in Southeast Alaska and its utilization for rheological parameter estimation

In Southeast Alaska (SE-AK), rapid ground uplift of up to 3 cm/yr has been observed associated with post-Little Ice Age glacial isostatic adjustment (GIA). Geodetic techniques such as global navigation satellite system (GNSS) and absolute gravimetry have been applied to monitor GIA since the last 1990s. Rheological parameters for SE-AK were determined from dense GNSS array data in earlier studies. However, the absolute gravity rate of change observed in SE-AK was inconsistent with the ground uplift rate, mainly because few gravity measurements from 2006 to 2008 resulted in imprecise gravity variation rates. Therefore, we collected absolute gravity data at six gravity points in SE-AK every June in 2012, 2013, and 2015, and updated the gravity variation rate by reprocessing the absolute gravity data collected from 2006 to 2015. We found that the updated gravity variation rate at the six gravity points ranged from −2.05 to −4.40 μGal/yr, and its standard deviation was smaller than that reported in the earlier study by up to 88 %. We also estimated the rheological parameters under the assumption of the incompressible Earth to explain the updated gravity variation rate, and their optimal values were determined to be 55 km and 1.2×10¹⁹ Pa s for lithospheric thickness and upper mantle viscosity, respectively. These optimal values are consistent with those independently obtained from GNSS observations, and this fact indicates that absolute gravimetry can be one of the most effective methods in determining sub-surface structural parameters associated with GIA accurately. Moreover, we utilized the gravity variation rates for estimating the ratio of gravity variation to vertical ground deformation at the six gravity points in SE-AK. The viscous ratio values were obtained as −0.168 and −0.171 μGal/mm from the observed data and the calculated result, respectively. These ratios are greater (in absolute) than those for other GIA regions (−0.15 to −0.16 μGal/mm in Antarctica and Fennoscandia), because glaciers in SE-AK have melted more recently than in other regions

Field measurements of absolute gravity in East Antarctica

This paper reports the results of field-based absolute gravity measurements aimed at detecting gravity change and crustal displacement caused by glacial isostatic adjustment. The project was initiated within the framework of the 53rd Japanese Antarctic Research Expedition (JARE53). Absolute gravity measurements, together with GPS measurements, were planned at several outcrops along the Prince Olav Coast and Sôya Coast of East Antarctica, including at Syowa Station. Since the icebreaker Shirase (AGB 5003) was unable to moor alongside Syowa Station, operations were somewhat restricted during JARE53. However, despite this setback, we were able to complete measurements at two sites: Syowa Station and Langhovde. The absolute gravity value at the Syowa Station IAGBN (A) site, observed using an FG-5 absolute gravimeter (serial number 210; FG-5 #210), was 982 524 322.7±0.1 μGal, and the gravity change rate at the beginning of 2012 was –0.26 μGal∙a-1. An absolute gravity value of 982 535 584.2±0.7 μGal was obtained using a portable A-10 absolute gravimeter (serial number 017; A-10 #017) at the newly located site AGS01 in Langhovde

Correlation study of observation data of polycyclic aromatic hydrocarbons and chemical transport model calculation data of other pollutants at Wajima

金沢大学大学院自然科学研究科場所：金沢大学自然科学研究科図書館棟1階，講演会場：図書館棟1階　大会議室，ポスター会場：図書館棟1階12会議室,主催・共催：文部科学省２１世紀COE「環日本海域の環境計測と長期・短期変動予測」， 大気環境学会， 金沢大学工学

Correlation study of observation data of polycyclic aromatic hydrocarbons and chemical transport model calculation data of other pollutants at Wajima

金沢大学大学院自然科学研究科場所：東京大学弥生講堂，共催：文部科学省２１世紀COE「環日本海域の環境計測と長期・短期変動予測」，大気環境学

High-Throughput Cryopreservation of Plant Cell Cultures for Functional Genomics

Suspension-cultured cell lines from plant species are useful for genetic engineering. However, maintenance of these lines is laborious, involves routine subculturing and hampers wider use of transgenic lines, especially when many lines are required for a high-throughput functional genomics application. Cryopreservation of these lines may reduce the need for subculturing. Here, we established a simple protocol for cryopreservation of cell lines from five commonly used plant species, Arabidopsis thaliana, Daucus carota, Lotus japonicus, Nicotiana tabacum and Oryza sativa. The LSP solution (2 M glycerol, 0.4 M sucrose and 86.9 mM proline) protected cells from damage during freezing and was only mildly toxic to cells kept at room temperature for at least 2 h. More than 100 samples were processed for freezing simultaneously. Initially, we determined the conditions for cryopreservation using a programmable freezer; we then developed a modified simple protocol that did not require a programmable freezer. In the simple protocol, a thick expanded polystyrene (EPS) container containing the vials with the cell–LSP solution mixtures was kept at −30°C for 6 h to cool the cells slowly (pre-freezing); samples from the EPS containers were then plunged into liquid nitrogen before long-term storage. Transgenic Arabidopsis cells were subjected to cryopreservation, thawed and then re-grown in culture; transcriptome and metabolome analyses indicated that there was no significant difference in gene expression or metabolism between cryopreserved cells and control cells. The simplicity of the protocol will accelerate the pace of research in functional plant genomics

Absolute Gravity Measurements and Gravity Changes at Syowa Station, Antarctica

第3回極域科学シンポジウム/第32回極域地学シンポジウム 11月29日（木） 統計数理研究所 ３階セミナー