Bioreduction of Solid Rocket Motors for Planetary Protection

Solid rocket propulsion systems have been used for in-space applications including planetary exploration missions for many years. Current NASA science lander projects require solid rocket propulsion systems to touchdown on the surface of potentially life-supporting planets and moons. A critical requirement of these missions is the prevention of accidental transportation of Earth's microbes to these environments. This mission requirement places an increased importance on the ability to reduce the biological burden that may be on board the solid propulsion systems and potentially deposited in a habitable environment. Some traditional interplanetary spacecraft decontamination operations could reduce the reliability of the solid propulsion system, indicating a need for new decontamination procedures. New techniques for biological burden reduction are being studied and may become the method of choice to ensure adequate reduction has been achieved. These techniques include biocidal elimination through chemical agents already present within the motor and cellular disruption due to assembly and operational environments induced in the motor. Recent investigations into the effectiveness of these techniques have generated promising experimental results. These techniques and current experimental results will be presented

Bioreduction of Solid Rocket Motors for Planetary Protection

No abstract availabl

Analysis of Bioreduction Strategies for a Solid Rocket Motor on an Interplanetary Mission to Europa

The Europa Lander De-Orbit stage braking motor must comply with the Planetary Protection requirements established for a category IV mission. In its mission to Europa, a motor that hasnt gone through bioreduction environments will carry microbial spores and other biosignature molecules that might jeopardize a mission of astrobiological concern as well as future missions to come. A motor with solid rocket propellant represents exclusive challenges associated to the calculation of high numbers of an encapsulated bioburden hidden behind a nozzle plug. Existing techniques for bioburden reduction are analysed in perspective to motor assembly facilities and the series of events that are involved in manufacturing a solid rocket motor. These techniques include antimicrobial effects of chemical components already present within the motor and bioreduction due to assembly and operational environments induced in the motor. Analysis of the manufacturing process, adhered bioburden and recent investigations into the effectiveness of microbiological techniques in finding inherent antimicrobial properties have generated a step by step outline of Planetary Protection for lander mission associated solid rocket motors

Biofilm formation is correlated with low nutrient and simulated microgravity conditions in a Burkholderia isolate from the ISS water processor assembly

The International Space Station (ISS) Water Processor Assembly (WPA) experiences intermittent dormancy in the WPA wastewater tank during water recycling events which promotes biofilm formation within the system. In this work we aimed to gain a deeper understanding of the impact of nutrient limitation on bacterial growth and biofilm formation under microgravity in support of biofilm mitigation efforts in exploration water recovery systems. A representative species of bacteria that is commonly cultured from the ISS WPA was cultured in an WPA influent water ersatz formulation tailored for microbiological studies. An isolate of Burkholderia contaminans was cultured under a simulated microgravity (SμG) treatment in a vertically rotating high-aspect rotating vessel (HARV) to create the low shear modeled microgravity (LSMMG) environment on a rotating wall vessel (RWV), with a rotating control (R) in the horizontal plane at the predetermined optimal rotation per minute (rpm) speed of 20. Over the course of the growth curve, the bacterial culture in ersatz media was harvested for bacterial counts, and transcriptomic and nutrient content analyses. The cultures under SμG treatment showed a transcriptomic signature indicative of nutrient stress and biofilm formation as compared to the R control treatment. Further analysis of the WPA ersatz over the course of the growth curve suggests that the essential nutrients of the media were consumed faster in the early stages of growth for the SμG treatment and thus approached a nutrient limited growth condition earlier than in the R control culture. The observed limited nutrient response may serve as one element to explain a moderate enhancement of adherent biofilm formation in the SμG treatment after 24 h. While nutrients levels can be modulated, one implication of this investigation is that biofilm mitigation in the ISS environment could benefit from methods such as mixing or the maintenance of minimum flow within a dormant water system in order to force convection and offset the response of microbes to the secondary effects of microgravity

Microbial isolation and characterization from two flex lines from the urine processor assembly onboard the International Space Station

Urine, humidity condensate, and other sources of non-potable water are processed onboard the International Space Station (ISS) by the Water Recovery System (WRS) yielding potable water. While some means of microbial control are in place, including a phosphoric acid/hexavalent chromium urine pretreatment solution, many areas within the WRS are not available for routine microbial monitoring. Due to refurbishment needs, two flex lines from the Urine Processor Assembly (UPA) within the WRS were removed and returned to Earth. The water from within these lines, as well as flush water, was microbially evaluated. Culture and culture-independent analysis revealed the presence of Burkholderia, Paraburkholderia, and Leifsonia. Fungal culture also identified Fusarium and Lecythophora. Hybrid de novo genome analysis of the five distinct Burkholderia isolates identified them as B. contaminans, while the two Paraburkholderia isolates were identified as P. fungorum. Chromate-resistance gene clusters were identified through pangenomic analysis that differentiated these genomes from previously studied isolates recovered from the point-of-use potable water dispenser and/or current NCBI references, indicating that unique populations exist within distinct niches in the WRS. Beyond genomic analysis, fixed samples directly from the lines were imaged by environmental scanning electron microscopy, which detailed networks of fungal-bacterial biofilms. This is the first evidence of biofilm formation within flex lines from the UPA onboard the ISS. For all bacteria isolated, biofilm potential was further characterized, with the B. contaminans isolates demonstrating the most considerable biofilm formation. Moreover, the genomes of the B. contaminans revealed secondary metabolite gene clusters associated with quorum sensing, biofilm formation, antifungal compounds, and hemolysins. The potential production of these gene cluster metabolites was phenotypically evaluated through biofilm, bacterial-fungal interaction, and hemolytic assays. Collectively, these data identify the UPA flex lines as a unique ecological niche and novel area of biofilm growth within the WRS. Further investigation of these organisms and their resistance profiles will enable engineering controls directed toward biofilm prevention in future space station water systems