Impact of terminal tip location of midline catheters on catheter‐related complications and indwelling duration in elderly patients

Abstract Aim To investigate the relationship between the position of the midline catheter tip and the frequency and type of complications associated with its placement. Design A cohort study was performed on patients between May 2018 and December 2021 who underwent midline catheter placement at our institution. Patients were divided into two groups, superficial implementation and deep implementation, based on the midline catheter tip location relative to the clavicle. Methods Clinical data and outcome parameters, including the numbers and types of midline catheter‐related complications, day of occurrence and catheter indwell duration, were recorded. Results Catheter‐related complications occurred in 14 individuals. Compared with the superficial implementation group, the incidence of complications in the deep catheterization group was significantly reduced, with a delayed first occurrence time, and a prolonged catheter indwelling time. The results suggested that locating the midline catheter tip in the distal segment of the axillary vein may reduce catheter‐related complication incidence and prolong the indwelling duration

Assessment of left ventricular systolic and diastolic abnormalities in patients with hypertrophic cardiomyopathy using real-time three-dimensional echocardiography and two-dimensional speckle tracking imaging

Abstract Background Conventional echocardiography is not sensitive enough to assess left ventricular (LV) dysfunction in hypertrophic cardiomyopathy (HCM) patients. This research attempts to find a new ultrasonic technology to better assess LV diastolic function, systolic function, and myocardial longitudinal and circumferential systolic strain of segments with different thicknesses in HCM patients. Methods This study included 50 patients with HCM and 40 healthy subjects as controls. The peak early and late mitral annulus diastolic velocities at six loci (Ea′ and Aa′, respectively) and the Ea′/Aa′ ratio were measured using real-time tri-plane echocardiography and quantitative tissue velocity imaging (RT-3PE-QTVI). The mean value of Ea′ at six loci (Em′) was obtained for the calculation of E/Em′ ratio. The LV end-diastolic volume (LVEDV), LV end-systolic volume (LVESV), LV stroke volume (LVSV), and LV ejection fraction (LVEF) were measured using real-time three-dimensional echocardiography (RT-3DE). LV myocardial longitudinal peak systolic strain (LPSS) and circumferential peak systolic strain (CPSS) in the apical-middle-basal segments (LPSS-api, LPSS-mid, LPSS-bas; CPSS-api, CPSS-mid, and CPSS-bas, respectively) were obtained using a software for two-dimensional speckle tracking imaging (2D-STI). According to the different segmental thicknesses (STs) in each HCM patient, the values (LPSS and CPSS) of all the myocardial segments were categorized into three groups and the respective averages were computed. Results The Ea′, Aa′, and, Ea′/Aa’ ratio in HCM patients were lower than those in the controls (all p < 0.001), while the E/Em′ ratio in HCM patients was higher than that in the controls (p < 0.001). The LVEDV, LVSV, and LVEF were significantly lower in HCM patients than in controls (all p < 0.001). In HCM patients, the LPSS-api, LPSS-mid, LPSS-bas, CPSS-api, CPSS-mid, and CPSS-bas and the LPSS and CPSS of LV segments with different thicknesses were all significantly reduced (all p < 0.001). Conclusions In HCM patients, myocardial dysfunction was widespread not only in the obviously hypertrophic segments but also in the non-hypertrophic segments; the LV systolic and diastolic functions were damaged, even with a normal LVEF. LV diastolic dysfunction, systolic dysfunction, and myocardial deformation impairment in HCM patients can be sensitively revealed by RT-3PE-QTVI, RT-3DE, and 2D-STI

Mechanistic study of incipient corrosion for nuclear grade lean-Cr FeCrAl alloys in a simulated PWR environment

This study systematically investigated the incipient corrosion behavior of Fe-13Cr-4.5Al (wt%) and lean-Cr (7–10 wt%) with 5Al (wt%) FeCrAl alloy with a timescale of 4 months under simulated PWR conditions (18.6 MPa and 360 °C) in an autoclave system. The corrosion induced two types of structure of oxide scale. FeCrAl alloys corroded for 3 months exhibited a duplex structure containing outer oxide particles and an inner Cr-rich spinel. During the corrosion test lasting 3–4 months, a triplex structure containing outer oxide particles, an inner compact Cr-rich spinel layer, and a porous spinel layer was observed. Moreover, Cr enriched at the periphery of outer oxides, indicating the formation of FeCr2O4 spinel oxide

Decision regret related to urinary diversion choices after cystectomy among Chinese bladder cancer patients

Abstract: Aim: To explore factors associated with decision regret after cystectomy among Chinese bladder cancer patients. Methods: This cross‐sectional study involved 112 patients, who had received radical bladder cancer resection. Participants were recruited from August 2021 until January 2022. The decision regret scale (DRS), decision conflict scale (DCS), and the Functional Assessment of Cancer Therapy–Bladder cancer (FACT‐BL) form were used to measure decision regret, decision conflict, and quality of life. Investigator‐designed items further explored perceptions involved in decision‐making participation and outcomes. Results: The average score for decision regret was 26.21 (SD 15.886), while decision conflict was 20.27 (SD 13.375) and quality of life was 94.74 (SD 20.873). 57.1% of our participants had a little knowledge about the quality of life of patients who chose an alternate urinary diversion method; however, only 13.4% reported having a clear understanding. In addition, 8.9%, 26.8%, and 36.6% thought that quality of life related to alternate decisions was poor, average, or good, respectively. Multiple regression analysis suggested that decision regret is associated with decision conflict, quality of life, and the perceptions that others (who took alternate urinary diversion decisions) had a better quality of life. Conclusion: Decision regret is common among Chinese bladder cancer patients, after cystectomy. The prevalence of regret appears to be much higher in Chinese bladder cancer patients compared to similar studies from other regions. Decisions in mainland China are often made by the treating physician or by family members which may cause more profound regret. However, education and economic status are positively related to higher levels of regret which creates questions around knowing, participation, and expectations, which must be explored

Additional file 3 of Comprehensive transcriptional variability analysis reveals gene networks regulating seed oil content of Brassica napus

Additional file 3. Review history

Additional file 2 of Comprehensive transcriptional variability analysis reveals gene networks regulating seed oil content of Brassica napus

Additional file 2: Table S1. Genome-wide eQTLs identified at 20 DAF. Table S2. Genome-wide eQTLs identified at 40 DAF. Table S3. Genome-wide eGene-eQTLs identified at 20 DAF. Table S4. Genome-wide eGene-eQTLs identified at 40 DAF. Table S5. Results of GO enrichment analysis of specifically identified eGenes at 20 DAF. Table S6. Results of GO enrichment analysis of specifically identified eGenes at 40 DAF. Table S7. Summary of ATAC-Seq data. Table S8. List of homoeologous genes among subgenomes. Table S9. List of homoeologous genes on gene expression trend and regulation at 20 DAF. Table S10. List of homoeologous genes on gene expression trend and regulation at 40 DAF. Table S11. The information of hotspots at 20 DAF. Table S12. The information of hotspots at 40 DAF. Table S13. The list of TWAS significant genes of SOC and SGC at 20 DAF. Table S14. The list of TWAS significant genes of SOC and SGC at 40 DAF. Table S15. Enrichment analysis of eQTL hotspot regulatory genes in TWAS significant genes of SOC at 20 DAF. Table S16. Enrichment analysis of eQTL hotspot regulatory genes in TWAS significant genes of SOC at 40 DAF. Table S17. The results of Tomtom analysis of key sequences identified by Basenji module. Table S18. Primers for gene cloning and PCR confirmation

Additional file 1 of Comprehensive transcriptional variability analysis reveals gene networks regulating seed oil content of Brassica napus

Additional file 1: Fig. S1. Overview of experimental and research analysis methods. Fig. S2. Venn diagram of the distribution of genes regulated by different types of eQTLs (local eQTL and distant eQTL) at 20 DAF and 40 DAF. (a) Distribution number of genes which were regulated by different types of eQTLs at 20 DAF. (b) Distribution number of genes which were regulated by different types of eQTLs at 40 DAF. Fig. S3. Manhattan plot of BnaA05.FAD7 eGWAS at 40 DAF. Fig. S4. Study design on ATAC-seq of 6 representative accessions of B. napus. Fig. S5. Correlation analysis of 59 ATAC-seq samples. The samples are named according to “(22, 26, 34 or 40) DAF” + “accession_cellular ploidy (2C, 3C or 4C)” + “biological replicate” format naming. Fig. S6. Regional plot of ATAC-seq data and eGWAS results of BnaA08.TGD1. BnaA08.TGD1 is marked by a dashed line. The shaded area indicates the lead SNP of the local eQTL affecting BnaA08.TGD1. Fig. S7. Comparison of the explained variance (r2) of eQTLs for expression variation in or not in OCRs. Fig. S8. Expression correlation analysis of adjacent genes and randomly sampled gene pairs. Violin plot shows that the expression correlation of adjacent genes is significantly higher than that of randomly sampled gene pairs, *** indicates P 0.05, * indicates P < 0.05, ** indicates P < 0.01 and *** indicates P < 0.001 compared with WT in Student’s t test. Fig. S34. Transcriptional regulation of genes are activated by BnaA09.SCL31 or BnaA07.NAC13. a Schematic representation of the constructs used for the dual-luciferase assay. The effector constructs contain BnaA09.SCL31 and BnaA07.NAC13 driven by the CaMV35S promoter, respectively. The reporter construct contains the firefly luciferase driven by BnaA07.NAC13, BnaC07.LPAAT, BnaC03.SLP1 and BnaA07.SRO3 promoter, and the Renilla luciferase (REN) driven by the CaMV35S promoter. And the black square is the terminator. b Bar graph showing the relative LUC/REN ratio in the dual-luciferase assay. Values are means(SD) (n = 3 biological repeats). BnaA07.SRO3 (SIMILAR TO RCD ONE 3)