Auditor of State Mary Mosiman today released a reaudit report on the Mason City Community School District (District) for the period July 1, 2014 through June 30, 2015. The reaudit also covered items applicable to the years ended June 30, 2016 and June 30, 2017.

Auditor of State Mary Mosiman today released a reaudit report on the Mason City Community School District (District) for the period July 1, 2014 through June 30, 2015. The reaudit also covered items applicable to the years ended June 30, 2016 and June 30, 2017

Primer registro de anomalía intersexual gonadal de Trachurus mediterraneus (Steindachner, 1868) desde el Mar de Alborán.

El objetivo principal de este trabajo es dar a conocer el primer registro de una anomalía intersexual gonadal de Trachurus mediterraneus desde el mar de Alborán (Mediterráneo occidental). Este espécimen es el primer registro de intersexualidad para un jurel en el mundo.Postprin

Low SIRT3 Expression Correlates with Poor Differentiation and Unfavorable Prognosis in Primary Hepatocellular Carcinoma

<div><p>SIRT3, a mitochondrial sirtuin belonging to nicotinamide adenine nucleotide (NAD) dependent deacetylases, is implicated in metabolism, longevity and carcinogenesis. SIRT3 expression and its significance in hepatocellular carcinoma (HCC) remain largely unclear. In this study, we demonstrated that SIRT3 expression in HCC tissue was much lower than that in paracarcinoma tissue, at both mRNA and protein levels. The cutoff value for low SIRT3 expression in HCC was defined according to receiver operating characteristic curve (ROC) analysis. As disclosed by immunohistochemistry (IHC) results, low SIRT3 expression was present in 67.3% (167/248) of HCC cases. Furthermore, low expression of SIRT3 was significantly correlated to differentiation (<em>P</em> = 0.013), clinical stage (<em>P</em> = 0.005), serum AFP level (<em>P</em><0.01), tumor multiplicity (<em>P</em> = 0.026) and relapse (<em>P</em> = 0.028). Moreover, Kaplan-Meier analysis indicated that low SIRT3 expression associated with unfavorable overall survival (<em>P</em><0.01) and recurrence-free survival (<em>P</em> = 0.004). The prognostic impact of SIRT3 was further confirmed by stratified survival analysis. Importantly, multivariate analysis revealed that low SIRT3 expression was an independent poor prognostic marker for overall survival (Hazard Ratio (HR) 0.555, 95% confidence interval (95% CI) 0.344–0.897, <em>P</em> = 0.016). Collectively, we conclude that SIRT3 is decreased in HCC and is a novel unfavorable marker for prognosis of patients with this fatal disease.</p> </div

Univariate analysis of SIRT3 expression and clinicopathologic variables in 248 patients with primary hepatocellular carcinoma (log-rank test).

a<p>Mean age;</p><p>NR, not reached; HbsAg, hepatitis B surface antigen; AFP, alpha-fetoprotein.</p

Reverse association of SIRT3 expression in HCC tissue with tumor differentiation.

<p><b>A.</b> Representative micrographic images were shown to present the correlation between SIRT3 expression in HCC and differentiation. <b>B.</b> Percentages of high SIRT3 expressions in differentiated HCC were indicated by histogram.</p

Protein expression patterns of SIRT3 in HCC tissues by IHC.

<p>The immunoreactivities were primarily observed in cytoplasm within tumor and normal liver cells. The micrographs showed negative (<b>A</b>), low (<b>C</b>), and high (<b>E</b>) expression of SIRT3 in HCC tissues. The relevant expressions of SIRT3 in corresponding adjacent normal liver tissues of cases in <b>A</b>, <b>C</b> and <b>E</b> were shown in <b>B</b>, <b>D</b> and <b>F</b>, respectively. (Left panel: magnification ×100; Right panel: magnification ×400.)</p

Correlation of low SIRT3 expression in HCC tissue with unfavorable overall survival and recurrence-free survival.

<p>Probabilities of overall survival <b>(A)</b> and recurrence-free survival <b>(B)</b> of total 248 HCC patients were analyzed by Kaplan-Meier survival analysis (log-rank test).</p

Correlation between the clinicopathologic variables and SIRT3 expression in HCC.

a<p>Chi-square test;</p>b<p>Mean age;</p><p>AFP, alpha-fetoprotein; HBsAg, hepatitis B surface antigen.</p

Cox multivariate analyses of prognostic factors on overall survival.

<p>β, Regression coefficient; SE, standard error; CI, confidence interval; AFP, alpha-fetoprotein.</p

Volume-rendered images show the relationship between the coronary sinus (CS) and mitral valve annulus (MVA).

<p>A. the CS is located along the MVA. B. the CS is located above the level of the MVA.; LA = left atrium; LV = left ventricle.</p