2,2′-[(1E)-3-Phenyl­prop-2-ene-1,1-di­yl]bis­(3-hy­droxy-5,5-dimethyl­cyclo­hex-2-en-1-one)

In the title mol­ecule, C25H30O4, the two cyclo­hexene rings adopt envelope conformations. The two hy­droxy groups are involved in the formation of intra­molecular O—H⋯O hydrogen bonds. In the crystal structure, weak inter­molecular C—H⋯O hydrogen bonds link mol­ecules related by translation along the axis a into chains

Rapid detection of sulfamethoxazole in plasma and food samples with in-syringe membrane SPE coupled with solid-phase fluorescence spectrometry

© 2020 Elsevier Ltd In this work, in-syringe membrane solid-phase extraction (MSPE) device was fabricated for the on-site sampling of sulfamethoxazole (SMX) in food samples followed by solid-phase fluorescence spectra analysis. The samples and fluorescamine (FA) were added to a syringe for derivation. Then, the derivative of SMX was extracted by a membrane in the syringe SPE device. Subsequently, the derivative on the membrane was measured immediately without additional elution procedure. The method was successfully applied in plasma, milk, and egg samples for the trace SMX detection, with the recovery of 98%–102%, RSDs from 1% to 6%. Compared with liquid chromatography, direct detection of the concentrated analyte significantly improved the sensitivity. Moreover, fluorescamine made it unnecessary to separate SMX from the interference. Consequently, it was a time-saving, low-cost, and easy-operation method, which demonstrated the potential of in-syringe SPE as a promising candidate for on-site analysis

Chitosan/Al\u3csub\u3e2\u3c/sub\u3eO\u3csub\u3e3\u3c/sub\u3e-HA nanocomposite beads for efficient removal of estradiol and chrysoidin from aqueous solution

© 2019 Elsevier B.V. Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work. The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis. FTIR spectra confirmed that the chitosan was loaded successfully on Al2O3-HA, and functional groups were immobilized onto the surface of the beads after the synthesis. The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments. Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics. The maximum adsorption capacity was found to be 39.78 mg/g and 23.26 mg/g for estradiol and chrysoidine, respectively. The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances. This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution

Stemness And Chemotherapeutic Drug Resistance Induced By Eif5a2 Overexpression In Esophageal Squamous Cell Carcinoma

Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies of the digestive tract in East Asian countries. Multimodal therapies, including adjuvant chemotherapy and neo-adjuvant chemotherapy, have become more often used for patients with advanced ESCC. However, the chemotherapy effect is often limited by patients' drug resistance. This study demonstrated that EIF5A2 (eukaryotic translation initiation factor 5A2) overexpression induced stemness and chemoresistance in ESCC cells. We showed that EIF5A2 overexpression in ESCC cells resulted in increased chemoresistance to 5-fluorouracil (5-FU), docetaxel and taxol. In contrast, shRNAs suppressing eIF5A2 increased tumor sensitivity to these chemotherapeutic drugs. In addition, EIF5A2 overexpression was correlated with a poorer overall survival in patients with ESCC who underwent taxane-based chemotherapy after esophagectomy (P > 0.05). Based on these results, we suggest that EIF5A2 could be a predictive biomarker for selecting appropriate chemo-treatment for ESCC patients and EIF5A2 inhibitors might be considered as combination therapy to enhance chemosensitivity in patients with ESCC.published_or_final_versio

A non-intrusive model order reduction approach for parameterized time-domain Maxwell's equations

International audienceWe present a non-intrusive model order reduction (NIMOR) method with an offline-online decoupling for the solution of parameterized time-domain Maxwell's equations. During the offline stage, the training parameters are chosen by using Smolyak sparse grid method with an approximation level L (L ≥ 1) over a target parameterized space. This method can deal with the so-called curse of dimensionality in high dimensional space. For each selected parameter, the snapshot vectors are first produced by a high order discontinuous Galerkin time-domain (DGTD) solver formulated on an unstructured simplicial mesh. In order to minimize the overall computational cost in the offline stage and to improve the accuracy of the NIMOR method, a radial basis function (RBF) interpolation method is then used to construct more snapshot vectors at the sparse grid with approximation level L + 1, which includes the sparse grids from approximation level L. A nested proper orthogonal decomposition (POD) method is employed to extract time-and parameter-independent POD basis functions. By using the singular value decomposition (SVD) method, the principal components of the reduced coefficient matrices of the high-fidelity solutions onto the reduced-order subspace spaned by the POD basis functions are extracted. Moreover, a Gaussian process regression (GPR) method is proposed to approximate the dominating time-and parameter-modes of the reduced coefficient matrices. During the online stage, the reduced-order solutions for new time and parameter values can be rapidly recovered via outputs from the regression models without using the DGTD method. The performance of this NIMOR method is illustrated numerically by considering two classical test cases: the scattering of a plane wave by a 2-D dielectric disk and the scattering of a plane wave by a multi-layer heterogeneous medium. The prediction capabilities of the NIMOR method are evaluated by varying the relative permittivity. Numerical results indicate that the NIMOR method is a promising approach for simulating accurately and in fast way parameterized timedomain electromagnetic problems

Effect of tea soil acidification on the diversity and function of fungi community

Soil fungi play an important role in the process of planting tea tree. However, effects of acidification on the diversity and function of the fungi community in tea rhizosphere soil have been rarely reported. In this study, tea rhizosphere soils with pH of 3.3, 4.7, 5.3 and 6.4 were investigated for diversity and function of fungal communities through T-RFLP technology. The results showed that the abundance and diversity of fungi increased significantly with the decrease of pH value of rhizosphere soil. The results of significance analysis showed that 38 T-RFs fragments were significantly correlated with pH value, among which 32 were negatively correlated with pH value and 6 were positively correlated with pH value. After database comparison, 23 fungi were identified and classified according to their nutritional patterns, which can be divided into four types, including pathotroph, symbiotroph, saprotroph and unknown, accounting for 36.85%, 7.89%, 15.79% and 39.47%, respectively. At pH value of 3.3, the fungus abundance reached the maximum value. In conclusion, acidification leads to changes in the structure and diversity of the fungi community in tea rhizosphere soil, specifically, a significant increase in the number and species of fungi, of which the pathotroph type is the largest. This study provides an important theoretical basis for controlling fungal diseases of tea tree in acidified tea plantation