SU6668 suppresses proliferation of triple negative breast cancer cells through down-regulating MTDH expression

BACKGROUND: The multiple tyrosine kinase inhibitors SU6668 have a promising therapeutic effect on the progression of hematological malignancies and some solid tumors. Here, we determined its effect on triple negative breast cancer (TNBC) cells and explored the potential molecular mechanism. METHODS: In this study, MDA-MB-231 cells were treated with SU6668 (15 μM, 30 μM) for 72 h and the change of proliferation was examined by MTT and tablet cloning. DNA ploidy was detected by flow cytometric analysis with PI staining. Double-label immunofluorescence method was used to detect the expression and distribution of MTDH proteins. VEGFR2, HIF-1α, MTDH, E-cadhrein, and SMA expressions were detected by Western bolt assay. RESULTS: This study showed that SU6668 inhibited the proliferation and induced polyploidization of MDA-MB-231 cells in a dose dependent form. SU6668 exposure increased the distribution of MTDH in cytoplasm and decreased its distribution in nuclei. After the treatment of SU6668, VEGFR2, HIF-1α, MTDH and SMA proteins were down-regulated, while E-cadhrein was up-regulated in MDA-MB-231 cells. CONCLUSIONS: In conclusion, SU6668 exposure maybe induces polyploidization, inhibit EMT and influence the expression of MTDH, which suppresses the proliferation in TNBC cells. MTDH is a key signal protein in downstream of VEGF/HIF-1αpathway in MDA-MB-231 cells, which may be used as the potential target in the treatment of TNBC

Changes and mechanisms of apparent resistivity before earthquakes of MS6.0–6.9 on the Chinese mainland

China has been conducting fixed continuous apparent resistivity observations since 1967. Up to June 2022, 45 earthquakes with magnitudes of MS6.0–6.9 have occurred within a range of approximately 250 km from normal operating stations. Through literature investigation and data analysis, monitoring stations counted 61 short-medium-term apparent resistivity anomalous changes (i.e., 44 decrease changes, 15 increase changes, and 2 perturbance changes) appearing before 39 of these earthquakes. In this study, we utilize a fault virtual dislocation model to understand the relative deformations around the epicenters before these earthquakes. The comparison results showed that 36 of the 44 decrease changes were in areas with compression enhancement and that 9 of the 15 increase changes were in areas with relative dilatancy. The results from rock petrophysical experiments and the resistivity model of the cracked medium showed decreased changes in the resistivity of water-bearing geomaterials during the successive loading of compressive stress, while the resistivity showed increased changes during the stress unloading process. Moreover, 45 of the 61 apparent resistivity anomalies were consistent with the mechanism of resistivity change under stress. These apparent resistivity anomalous changes before earthquakes may be related to the seismogenic processes such that the resistivity change is caused by medium deformation

Transportation dynamics on networks of mobile agents

Most existing works on transportation dynamics focus on networks of a fixed structure, but networks whose nodes are mobile have become widespread, such as cell-phone networks. We introduce a model to explore the basic physics of transportation on mobile networks. Of particular interest are the dependence of the throughput on the speed of agent movement and communication range. Our computations reveal a hierarchical dependence for the former while, for the latter, we find an algebraic power law between the throughput and the communication range with an exponent determined by the speed. We develop a physical theory based on the Fokker-Planck equation to explain these phenomena. Our findings provide insights into complex transportation dynamics arising commonly in natural and engineering systems

Metabolome and Transcriptome Analyses Unravels Molecular Mechanisms of Leaf Color Variation by Anthocyanidin Biosynthesis in Acer triflorum

Acer triflorum Komarov is an important ornamental tree, and its seasonal change in leaf color is the most striking feature. However, the quantifications of anthocyanin and the mechanisms of leaf color change in this species remain unknown. Here, the combined analysis of metabolome and transcriptome was performed on green, orange, and red leaves. In total, 27 anthocyanin metabolites were detected and cyanidin 3-O-arabinoside, pelargonidin 3-O-glucoside, and peonidin 3-O-gluside were significantly correlated with the color development. Several structural genes in the anthocyanin biosynthesis process, such as chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR), were highly expressed in red leaves compared to green leaves. Most regulators (MYB, bHLH, and other classes of transcription factors) were also upregulated in red and orange leaves. In addition, 14 AtrMYBs including AtrMYB68, AtrMYB74, and AtrMYB35 showed strong interactions with the genes involved in anthocyanin biosynthesis, and, thus, could be further considered the hub regulators. The findings will facilitate genetic modification or selection for further improvement in ornamental qualities of A. triflorum

Certain Class of Analytic Functions Based on qq-difference operator

In this paper, we considered a generalized class of starlike functions defined by Kanas and R\u{a}ducanu\cite{10} to obtain integral means inequalities and subordination results. Further, we obtain the for various subclasses of starlike functions.Comment:

Chromosome-Level Genome Assembly for Acer pseudosieboldianum and Highlights to Mechanisms for Leaf Color and Shape Change

Acer pseudosieboldianum (Pax) Komarov is an ornamental plant with prominent potential and is naturally distributed in Northeast China. Here, we obtained a chromosome-scale genome assembly of A. pseudosieboldianum combining HiFi and Hi-C data, and the final assembled genome size was 690.24 Mb and consisted of 287 contigs, with a contig N50 value of 5.7 Mb and a BUSCO complete gene percentage of 98.4%. Genome evolution analysis showed that an ancient duplication occurred in A. pseudosieboldianum. Phylogenetic analyses revealed that Aceraceae family could be incorporated into Sapindaceae, consistent with the present Angiosperm Phylogeny Group system. We further construct a gene-to-metabolite correlation network and identified key genes and metabolites that might be involved in anthocyanin biosynthesis pathways during leaf color change. Additionally, we identified crucial teosinte branched1, cycloidea, and proliferating cell factors (TCP) transcription factors that might be involved in leaf morphology regulation of A. pseudosieboldianum, Acer yangbiense and Acer truncatum. Overall, this reference genome is a valuable resource for evolutionary history studies of A. pseudosieboldianum and lays a fundamental foundation for its molecular breeding

Pressure-induced superconductivity in charge-density-wave compound LaTe2-xSbx (x=0 and 0.4)

Here, we have grown single crystals of LaTe2-xSbx (x=0 and 0.4) with continuously adjustable CDW. High-pressure x-ray diffraction show LaTe2 does not undergo phase transition and keep robust below 40 GPa. In-situ high-pressure electrical measurements show LaTe2-xSbx undergo semiconductor-metal-superconductivity transition at 4.6 and 2.5 GPa, respectively. With the doping of Sb, the highest Tc increases from 4.6 to 6.5 K. Theoretical calculations reveal that the CDW has been completely suppressed and the calculated Tc is about 2.97 K at 4.5 GPa, consistent with the measured value. Then, the pressure-induced superconductivity in LaTe2-xSbx can be explained in the framework of the BCS theory.Comment: 11 pages, 5 figure

Heating of multi‐species upflowing ion beams observed by Cluster on March 28, 2001

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/149495/1/epp320083.pd

Capsule membranes encapsulated with smart nanogels for facile detection of trace lead(II) ions in water

A novel method based on capsule membranes encapsulated with smart nanogels is successfully developed for facilely detecting trace lead(II) (Pb2+) ions, which are hazardous to both human health and the environment because of their toxicity. The capsule membrane system is composed of a semi-permeable calcium alginate membrane and encapsulated poly(N-isopropylacrylamide-co-acryloylamidobenzo-18-crown-6) (PNB) nanogels. The semi-permeable membrane allows Pb2+ ions and water to pass through quickly, but rejects the encapsulated nanogels and polymers totally. As soon as Pb2+ ions appear in the aqueous environment and enter into the capsule, they can be specifically recognized by encapsulated PNB nanogels via forming 18-crown-6/Pb2+ complexes that cause a Pb2+-induced phase transition of PNB nanogels from hydrophobic to hydrophilic state. As a result, the osmotic pressure inside the capsule membrane increases remarkably, and thus the elastic capsule membrane isothermally swells upon the presence of Pb2+ ions in the environmental aqueous solution. The Pb2+-induced swelling degree of the capsule membrane is dependent on the concentration of Pb2+ ions ([Pb2+]) in water. Thus, the [Pb2+] value in water is able to be easily detected by directly measuring the Pb2+-induced isothermal swelling ratio of the capsule membrane, which we demonstrate by using 15 prepared capsule membranes arranged in a line. The Pb2+-induced swelling ratios of the capsule membrane groups are easily observed with the naked eye, and the detection limit of the [Pb2+] in water is 10-9 mol L-1. Such a proposed method provides an easy and efficient strategy for facile detection of trace threat analytes in water

Biobutanol production in a Clostridium acetobutylicum biofilm reactor integrated with simultaneous product recovery by adsorption

BACKGROUND: Clostridium acetobutylicum can propagate on fibrous matrices and form biofilms that have improved butanol tolerance and a high fermentation rate and can be repeatedly used. Previously, a novel macroporous resin, KA-I, was synthesized in our laboratory and was demonstrated to be a good adsorbent with high selectivity and capacity for butanol recovery from a model solution. Based on these results, we aimed to develop a process integrating a biofilm reactor with simultaneous product recovery using the KA-I resin to maximize the production efficiency of biobutanol. RESULTS: KA-I showed great affinity for butanol and butyrate and could selectively enhance acetoin production at the expense of acetone during the fermentation. The biofilm reactor exhibited high productivity with considerably low broth turbidity during repeated batch fermentations. By maintaining the butanol level above 6.5 g/L in the biofilm reactor, butyrate adsorption by the KA-I resin was effectively reduced. Co-adsorption of acetone by the resin improved the fermentation performance. By redox modulation with methyl viologen (MV), the butanol-acetone ratio and the total product yield increased. An equivalent solvent titer of 96.5 to 130.7 g/L was achieved with a productivity of 1.0 to 1.5 g · L(-1) · h(-1). The solvent concentration and productivity increased by 4 to 6-fold and 3 to 5-fold, respectively, compared to traditional batch fermentation using planktonic culture. CONCLUSIONS: Compared to the conventional process, the integrated process dramatically improved the productivity and reduced the energy consumption as well as water usage in biobutanol production. While genetic engineering focuses on strain improvement to enhance butanol production, process development can fully exploit the productivity of a strain and maximize the production efficiency