Digital transformation: information system governance

Effects of MEG2 and miR-181a-5p on the growth of gastric cancer xenografted tumours in vivo. a Quantitative analysis of western blot analysis of MEG2 protein expression levels in xenografted tumours. b H&E and immunohistochemical staining for Ki-67 in xenografted tumours. ** P < 0.01. (TIFF 1211 kb

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Additional file 3: Figure S3. of ING5 suppresses breast cancer progression and is regulated by miR-24

Western blot analysis of the expression levels of ING5 protein in MCF-7 cells. (A-B) Western blot analysis of the expression levels of ING5 protein in MCF-7 cells co-transfected with pre-miR-control plus control plasmid, pre-miR-24 plus control plasmid, pre-miR-control plus an ING5-HA overexpressing plasmid, or pre-miR-24 plus an ING5-HA overexpressing plasmid. A: representative image; B: quantitative analysis. UD: undetected; **p < 0.01; ***p < 0.001. (TIF 144 kb

Additional file 1: Table S1. of The Jun/miR-22/HuR regulatory axis contributes to tumourigenesis in colorectal cancer

Clinical features of colorectal cancer patients. (DOCX 17 kb

Additional file 3: Figure S1. of The Jun/miR-22/HuR regulatory axis contributes to tumourigenesis in colorectal cancer

HuR protein is significantly upregulated in CRC tissues and negatively correlated with CRC patient survival. (a) HuR levels in normal colon, normal rectum, colon adenocarcinoma and rectal adenocarcinoma in the TCGA dataset analysed by Oncomine. (b) Kaplan-Meier curve showing the negative correlation of HuR level and CRC patients’ survival. (TIFF 167 kb

Additional file 5: Figure S3. of The Jun/miR-22/HuR regulatory axis contributes to tumourigenesis in colorectal cancer

HuR functions as an oncogene in CRC. (a-c) HuR promoted SW480 proliferation. a: CCK-8 assays; b and c: EdU assays. (d and e) HuR promoted SW480 migration. (f-h) HuR accelerated CRC xenografted tumour growth. f: Photos of CRC tumours; g: Tumour volume curves; h: Tumour weights. (i) Western blot analysis of HuR levels in CRC xenografted tumours. (j and k) HE staining and IHC staining for HuR and Ki-67 in xenografted tumours. **P < 0.01; ***P < 0.001. (TIFF 5991 kb

Additional file 6: Figure S4. of The Jun/miR-22/HuR regulatory axis contributes to tumourigenesis in colorectal cancer

(a and b) The Kaplan-Meier curve revealed the positive correlation of miR-22 (a) or miR-129 (b) level and CRC patients’ survival. (c and d) The transfection efficiencies of miR-22 (c) or miR-129 (d) mimics or inhibitors in 3 CRC cell lines. ***P < 0.001. (TIFF 1147 kb

Additional file 4: Figure S2. of The Jun/miR-22/HuR regulatory axis contributes to tumourigenesis in colorectal cancer

The efficiencies of siRNA and overexpression vector of HuR (a) or Jun (b). (TIFF 377 kb