The Study of the Composition of Chloroform Fraction of Anemone nemorosa L.

Expanding the range of herbal drugs is among the priorities of the modern pharmacy. The analysis presented in this article is drawn from a broader qualitative study examining the composition of Anemone nemorosa L. chloroform fraction. The study revealed 38 compounds, and 32 of them were identified. The investigated lipophilic extract has an antimicrobial activity both in terms of gram-positive and gram-negative microorganisms. The maximal antimicrobial action of the investigated extract was shown to Escherichia coli. The significant content of biologically active substances in the lipophilic volatile fraction of Anemone nemorosa indicates the prospects for further study

Three serendipitous pathways in E. coli can bypass a block in pyridoxal-5′-phosphate synthesis

Overexpression of seven different genes restores growth of a ΔpdxB strain of E. coli, which cannot make pyridoxal phosphate (PLP), on M9/glucose.None of the enzymes encoded by these genes has a promiscuous 4-phosphoerythronate dehydrogenase activity that can replace the activity of PdxB.Overexpression of these genes restores PLP synthesis by three different serendipitous pathways that feed into the normal PLP synthesis pathway downstream of the blocked step.Reactions in one of these pathways are catalyzed by low-level activities of enzymes of unknown function and a promiscuous activity of an enzyme that normally has a role in another pathway; one reaction appears to be non-enzymatic