The comparision of glybenclamide and metformin-loaded bacterial cellulose/gelatin nanofibres produced by a portable electrohydrodynamic gun for diabetic wound healing

Wound dressings made from natural polymers are an important aspect of biomaterials. Protein-based materials are less likely to instigate an immunogenic response and have the capacity to degrade in vivo, also without triggering an inflammatory response. Therefore, gelatin (GEL) was chosen and combined with bacterial cellulose (BC) to produce nanofibres and the potential of an all-natural polymer construct was determined. GEL and BC were successfully electrospun with metformin (Met) and glybenclamide (Gb) using a portable, point of need electrospinning set up. The virgin fibre group exhibited a significant effect on the proliferation of L929 (mouse fibroblast) cells but all fibre samples can safely be applied on wound site without risk of cytotoxicity. According to the results obtained by animal tests, the GEL-BC-Gb group showed better recovery than the GEL-BC-Met group. Diabetic wounds treated with GEL-BC-Met were characterized by moderate re-epithelialization and partially organized granulation tissue. Moderate to complete re-epithelialization and well-formed granulation tissue were observed in diabetic wounds treated with GEL-BC-Gb. The histologic scores obtained on day 14 confirmed that the GEL-BC-Gb group played a stronger wound-healing role compared to the GEL-BC-Met group. The highest decrease of TNF-α level was observed in the GEL-BC-Gb group at the end of the experiment but there is no significant difference between drug-loaded fibre groups. Therefore, topical administration of Met and Gb in a sustained release form has a high potential for diabetic wound healing with high bioavailability and fewer systemic side effects but Gb showed better improvement according to the results of the animal tests

Peripheral administration of lactate produces antidepressant-like effects.

In addition to its role as metabolic substrate that can sustain neuronal function and viability, emerging evidence supports a role for l-lactate as an intercellular signaling molecule involved in synaptic plasticity. Clinical and basic research studies have shown that major depression and chronic stress are associated with alterations in structural and functional plasticity. These findings led us to investigate the role of l-lactate as a potential novel antidepressant. Here we show that peripheral administration of l-lactate produces antidepressant-like effects in different animal models of depression that respond to acute and chronic antidepressant treatment. The antidepressant-like effects of l-lactate are associated with increases in hippocampal lactate levels and with changes in the expression of target genes involved in serotonin receptor trafficking, astrocyte functions, neurogenesis, nitric oxide synthesis and cAMP signaling. Further elucidation of the mechanisms underlying the antidepressant effects of l-lactate may help to identify novel therapeutic targets for the treatment of depression

Transportation of microplastic during high-flow and low-flow seasons in southeastern Black Sea: A modelling approach

663-670Predicting the transportation patterns of microplastic particles is crucial for assessing the environmental risks. The present work studied the transportation and accumulation of floating microplastic particles released from the largest river basin (Değirmendere), on the southeastern Black Sea coast. A total of 2 scenarios were simulated for high-flow and low-flow seasons to predict the effect of freshwater input on the concentration, transportation and accumulation patterns of microplastic particles by using a Lagrangian model; the Estuarine, Coastal Ocean Model with Sediment Transport (ECOMSED) model. Microplastic concentration varied between 168 – 1412 particle/m3 and 0 – 843 particle/m3 in the high-flow and low-flow seasons, respectively. Microplastic particles released from the river showed different accumulation patterns with time. However, the particles tended to accumulate on coastal waters in both scenarios. The present study provides a baseline for determining the hotspots for the accumulation of floating microplastic particles to assess optimal sampling locations and risk assessment in the southeastern Black Sea

P.4.c.003 Resveratrol shows vasoprotective effect in an unpredictable chronic mild stress model of depression in rats

27th Congress of the European-College-of-Neuropsychopharmacology (ECNP) -- OCT 18-21, 2014 -- Berlin, GERMANY[No Abstract Available]European Coll Neuropsychopharmaco