Polymorphism of the testis-specific serine/threonine kinase 2 gene and risk of asthenozoospermia in Côte d'Ivoire

Background: The testis-specific serine/threonine protein kinase (TSSK2) is an indispensable protein responsible for the mobility of spermatozoa expressed specifically in the germ cells during spermatogenesis and present in the mature spermatozoa. Its gene mutation could constitute a risk of infertility. The aim of this study is to investigate the polymorphism of this TSSK2 gene in men with asthenozoospermia.Methods: The ejaculates were obtained from patients attending the reproductive biology unit of Institut Pasteur of Côte d’Ivoire for their spermiological evaluations. The semen analyses are performed with the automatic sperm analyzer SQA-Vision. 30 sperms, including 20 asthenozoosperms and 10 normosperms, were selected from their spermiological results and the spermatozoa DNA was extracted by the phenol/chloroform method. Direct Sequencing of the spermatozoa DNA fragments was done using the Sanger method. The frequencies of mutation were analysis with the Fisher and Mann-Whitney tests.   Results: It was revealed 17 mutations in 22 ejaculates. The frequent mutations are c.839C>T (T280M), c.816G>C (L372L), c.1026G>A (R342R), c.785A>C (H262P) and c.80A>G (K27R) with respectively frequencies of 50.0%, 26.67%, 16.67%, 13.33% and 10.0%. The analysis of these mutations indicated a significant difference in the frequency of occurrence of mutations between normosperms and asthenozoosperms (p-value = 0.01).Conclusions: This study shows that mutations in the TSSK2 gene are more common in asthenozoosperm ejaculates than normosperm ejaculates. This fact suggests the probable association of mutations in the TSSK2 gene with asthenozoospermia

Ghana

Including inland water bodies, Ghana covers 238,539 square kilometers and is located on the south central coast of West Africa. The country shares borders in the east with Togo, in the north with Burkina Faso, and in the west with Côte d’Ivoire. The topography of Ghana is mainly undulating, with most slopes less than 5 percent and many not exceeding 1 percent. The topography of the high rainforest is, however, mainly strongly rolling. The uplifted edges of the Voltarian basin give rise to narrow plateaus between 300 and 600 meters in elevation (Boateng 1998). Moving from the rainforest zone in the south to the Sahara Desert in the north, rainfall generally decreases and temperature increases. Rainfall is the most important climatic factor influencing vegetation in Ghana. The wettest area is in the extreme southwest, where the rainfall is over 2,000 millimeters per year. In the extreme north, the annual rainfall is less than 1,100 millimeters. The driest area is at the southeastern coastal tip, where the rainfall is about 750 millimeters. Much of the rain falls in intense storms of short duration, especially at the beginning of the season, resulting in heavy runoff and erosion. The annual mean relative humidity is about 80 percent in the south and 44 percent in the north (Dickson and Benneh 1988). The mean monthly temperature for the entire country is 25°C. Although temperatures are uniformly moderate, there are important variations over different parts of the country, reflecting altitude and distance from the sea..

Niger

Niger is a landlocked country in West Africa located between 11°37´ and 23°23´ north latitude and between 00°10´ and 16°00´ east longitude, with an area of 1,267,000 square kilometers. Niger shares borders with Algeria and Libya in the north, Chad in the east, Nigeria and Benin in the south, and Burkina Faso and Mali in the west. Three-fourths of Niger is covered by the Sahara Desert. The southern part of the country is in the Sahelian climate zone, with Sudan savannah vegetation. The rainy season lasts for only three months, with total rainfall ranging from 150 to 600 millimeters per year in the Sudan savannah; maximum temperatures are high (45°C in the shade in April–May). The vegetation cover is sparse, and nomadic agriculture is dominant..

Burkina Faso

Burkina Faso is a landlocked country in West Africa covering about 274,000 square kilometers. It is bordered by the Republic of Mali on the north and west; by Cote d’Ivoire on the Southwest; by Ghana, Togo, and Benin on the South; and by Niger on the east. The country has a dry tropi¬cal climate with two contrasting seasons. The rainy season generally lasts from May to October, but its duration decreases progressively from the southwest, amounting to only three months in the northern part of the country. Agriculture accounts for 40 percent of the gross domestic product (GDP) and 60 percent of the total exports of Burkina Faso. Its cropped area is 3.5–4.0 million hectares, representing about 13 percent of the country’s total area and one-third of the arable land. Rainfed agriculture dominates, with largely rudimentary agricultural techniques prevailing among small-scale farmers. Crop production is more diversified in the Sudanian zone (in the southwest), with a variety of roots and tubers (yams, sweet potatoes, and cocoyams), fruits (mangoes, bananas, and citrus fruits), cashews, and sugarcane. The major cash crops are cotton, groundnuts, cowpeas, and sesame

Improving the sustainability and effectiveness of photovoltaic evaporative cooling greenhouse in the Sahel

Abstract Anthropogenic climate change has caused worldwide extreme weather events including droughts, floods and heatwaves. It disproportionately affects developing countries through food insecurity. Greenhouse is important and relevant to the food-energy-water security in many regions. This study investigates the thermal behavior of photovoltaic evaporative cooling greenhouse made with eco-friendly coolers. The cooling potential of local plant materials was assessed under ambient conditions. Experimental thermal data obtained from optimized evaporative cooling system equipped with Hyphaene thebaica fibers (HF-pad) and conventional Celdek pad (C-pad), were used in heat and mass transfer equations to derive the greenhouse cooling performances. Computational fluid dynamics analysis software was used to investigate the refrigerant fluid distribution in the greenhouse. Cooler using HF-pad allows to keep the microclimate below 25 °C, with maximum moisture rate up to 80%, under harsh ambient conditions (temperature: 30–45 °C, humidity: 10–15%). HF-pad had the highest cooling coefficient of performance (COP = 9 against 6 for C-pad), the best cost to efficiency ratio (CER = 5; 4 times less than C-pad) and the lowest outlet temperature (20.0 °C). Due to higher outlet air velocity (1.116 m/s against 0.825 m/s for HF-pad), C-pad cooler spread cool air (20.5 °C) up to 1.25 m farther than its counterpart, creating higher pressure in the atmosphere (1.42 Pa against 0.71 Pa), with 2 times turbulent kinetic energy (0.014 J/kg). HF-pad presented cooling performances that compete with conventional pads. Moreover, optimization of HF-pad frame engineering and the technology scaling up to industrial level can allow better thermal and economic performances

Dynamiques d'occupation et d'utilisation des sols et construction des innovations dans les cuvettes de maraichage du gandiolais (Niayes)

Trabajo presentado en la 3ème édition de la Conférence Intensification Durable (CID), celebrada en Dakar del 24 al 26 de noviembre de 2021

Amélioration de la productivité agricole des systèmes irrigués dans la zone Nord des Niayes

Trabajo presentado en la 3ème édition de la Conférence Intensification Durable (CID), celebrada en Dakar del 24 al 26 de noviembre de 2021