Experiments and analyses of upstream-advancing solitary waves generated by moving disturbances

In this joint theoretical, numerical and experimental study, we investigate the phenomenon of forced generation of nonlinear waves by disturbances moving steadily with a transcritical velocity through a layer of shallow water. The plane motion considered here is modelled by the generalized Boussinesq equations and the forced Korteweg-de Vries (fKdV) equation, both of which admit two types of forcing agencies in the form of an external surface pressure and a bottom topography. Numerical results are obtained using both theoretical models for the two types of forcings. These results illustrate that within a transcritical speed range, a succession of solitary waves are generated, periodically and indefinitely, to form a procession advancing upstream of the disturbance, while a train of weakly nonlinear and weakly dispersive waves develops downstream of an ever elongating stretch of a uniformly depressed water surface immediately behind the disturbance. This is a beautiful example showing that the response of a dynamic system to steady forcing need not asymptotically tend to a steady state, but can be conspicuously periodic, after an impulsive start, when the system is being forced at resonance. A series of laboratory experiments was conducted with a cambered bottom topography impulsively started from rest to a constant transcritical velocity U, the corresponding depth Froude number F = U/(gh[sub]0)^1/2 (g being the gravitational constant and h[sub]0 the original uniform water depth) being nearly the critical value of unity. For the two types of forcing, the generalized Boussinesq model indicates that the surface pressure can be more effective in generating the precursor solitary waves than the submerged topography of the same normalized spatial distribution. However, according to the fKdV model, these two types of forcing are entirely equivalent. Besides these and some other rather refined differences, a broad agreement is found between theory and experiment, both in respect of the amplitudes and phases of the waves generated, when the speed is nearly critical (0.9 F > 0.2, finally disappear at F ~= 0.2. In the other direction, as the Froude number is increased beyond F ~= 1.2, the precursor soliton phenomenon was found also to evanesce as no finite-amplitude solitary waves can outrun, nor can any two-dimensional waves continue to follow, the rapidly moving disturbance. In this supercritical range and for asymptotically large times, all the effects remain only local to the disturbance. Thus, the criterion of the fascinating phenomenon of the generation of precursor solitons is ascertained

An X-ray Spectral Survey of Radio-Loud AGN With ASCA

We present a uniform and systematic analysis of the 0.6-10 keV X-ray spectra of radio-loud active galactic nuclei (AGN) observed by ASCA. The sample 10 BLRGs, 5 QSRs, 9 NLRGs, and 10 RGs. At soft X-rays, about half of the NLRGs and all of the RGs exhibit a thermal plasma component, with a bimodal distribution of temperatures and luminosities, suggesting an origin either in a surrounding cluster or loose group or in a hot corona. At energies above 2 keV, a hard power-law component is detected in 90% of cases. The power-law photon indices and luminosities in BLRGs, QSRs, and NLRGs are similar, consistent with orientation-based unification schemes. Significant excess cold absorption is detected in most NLRGs, but also in some BLRGS and QSRs, which was somewhat unexpected. In contrast to Seyfert galaxies, only one object showss the signature of a warm absorber. The nuclear X-ray luminosity is correlated with the luminosity of the [O III] emission line, the FIR emission at 12 microns, and the lobe radio power at 5 GHz. The Fe K line is detected in 50% of BLRGs, one QSR, and a handful of NLRGs. This sample also includes 6 Weak Line Radio Galaxies (WLRGs). Their spectra WLRGs can be generally decomposed into a soft thermal component with hard absrorbed power-law component, which is significantly flatter than any other radio-loud AGNs. Their intrinsic luminosities are two orders of magnitude lower than in other sources of the sample. An interesting possibility is that WLRGs represent an extreme population of radio galaxies in which the central black hole is accreting at a rate well below the Eddington rate.Comment: To appear in the Astrophysical Journal. 72 pages, including many tables and figures. Fig 1 is separate, in TIFF format. Postscript version of fig 1 and postscript version of entire preprint can be obtained from http://www.astro.psu.edu/users/mce/preprint_index.htm

Reconstruction of cell population dynamics using CFSE

Background: Quantifying cell division and death is central to many studies in the biological sciences. The fluorescent dye CFSE allows the tracking of cell division in vitro and in vivo and provides a rich source of information with which to test models of cell kinetics. Cell division and death have a stochastic component at the single-cell level, and the probabilities of these occurring in any given time interval may also undergo systematic variation at a population level. This gives rise to heterogeneity in proliferating cell populations. Branching processes provide a natural means of describing this behaviour. Results: We present a likelihood-based method for estimating the parameters of branching process models of cell kinetics using CFSE-labeling experiments, and demonstrate its validity using synthetic and experimental datasets. Performing inference and model comparison with real CFSE data presents some statistical problems and we suggest methods of dealing with them. Conclusion: The approach we describe here can be used to recover the (potentially variable) division and death rates of any cell population for which division tracking information is available

A Novel Role of Peripheral Corticotropin-Releasing Hormone (CRH) on Dermal Fibroblasts

Corticotropin-releasing hormone, or factor, (CRH or CRF) exerts important biological effects in multiple peripheral tissues via paracrine/autocrine actions. The aim of our study was to assess the effects of endogenous CRH in the biology of mouse and human skin fibroblasts, the primary cell type involved in wound healing. We show expression of CRH and its receptors in primary fibroblasts, and we demonstrate the functionality of fibroblast CRH receptors by induction of cAMP. Fibroblasts genetically deficient in Crh (Crh−/−) had higher proliferation and migration rates and compromised production of IL-6 and TGF-β1 compared to the wildtype (Crh+/+) cells. Human primary cultures of foreskin fibroblasts exposed to the CRF1 antagonist antalarmin recapitulated the findings in the Crh−/− cells, exhibiting altered proliferative and migratory behavior and suppressed production of IL-6. In conclusion, our findings show an important role of fibroblast-expressed CRH in the proliferation, migration, and cytokine production of these cells, processes associated with the skin response to injury. Our data suggest that the immunomodulatory effects of CRH may include an important, albeit not explored yet, role in epidermal tissue remodeling and regeneration and maintenance of tissue homeostasis

A stubbornly large mass of cold dust in the ejecta of Supernova 1987A

We present new Herschel photometric and spectroscopic observations of Supernova 1987A, carried out in 2012. Our dedicated photometric measurements provide new 70 micron data and improved imaging quality at 100 and 160 micron compared to previous observations in 2010. Our Herschel spectra show only weak CO line emission, and provide an upper limit for the 63 micron [O I] line flux, eliminating the possibility that line contaminations distort the previously estimated dust mass. The far-infrared spectral energy distribution (SED) is well fitted by thermal emission from cold dust. The newly measured 70 micron flux constrains the dust temperature, limiting it to nearly a single temperature. The far-infrared emission can be fitted by 0.5+-0.1 Msun of amorphous carbon, about a factor of two larger than the current nucleosynthetic mass prediction for carbon. The observation of SiO molecules at early and late phases suggests that silicates may also have formed and we could fit the SED with a combination of 0.3 Msun of amorphous carbon and 0.5 Msun of silicates, totalling 0.8 Msun of dust. Our analysis thus supports the presence of a large dust reservoir in the ejecta of SN 1987A. The inferred dust mass suggests that supernovae can be an important source of dust in the interstellar medium, from local to high-redshift galaxies.Comment: ApJ accepted, 8 page

2017 American College of Rheumatology/American Association of Hip and Knee Surgeons Guideline for the Perioperative Management of Antirheumatic Medication in Patients With Rheumatic Diseases Undergoing Elective Total Hip or Total Knee Arthroplasty

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137753/1/acr23274.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137753/2/acr23274_am.pd

2017 American College of Rheumatology/American Association of Hip and Knee Surgeons Guideline for the Perioperative Management of Antirheumatic Medication in Patients With Rheumatic Diseases Undergoing Elective Total Hip or Total Knee Arthroplasty

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137769/1/art40149.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/137769/2/art40149_am.pd

Mitochondrial Lactate Dehydrogenase Is Involved in Oxidative-Energy Metabolism in Human Astrocytoma Cells (CCF-STTG1)

Lactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP via oxidative phosphorylation. 13C-NMR and HPLC analyses aided in the identification of tricarboxylic acid (TCA) cyle metabolites and ATP in the astrocytic mitochondria incubated with lactate. Oxamate, an inhibitor of lactate dehydrogenase (LDH), abolished mitochondrial lactate consumption. Electrophoretic and fluorescence microscopic analyses helped localize LDH in the mitochondria. Taken together, this study implicates lactate as an important contributor to ATP metabolism in the brain, a finding that may significantly change our notion of how this important organ manipulates its energy budget