Pluripotency of mesenchymal cells derived from synovial fluid in patients with temporomandibular joint disorder.

Mesenchymal stem cells are an interesting source of material for regenerative medicine. The present study aimed at characterizing the phenotype and differentiation potential of adherent synovial fluid-derived cells from temporomandibular joint (TMJ) disorder patients

Orchestrated ensemble activities constitute a hippocampal memory engram

The brain stores and recalls memories through a set of neurons, termed engram cells. However, it is unclear how these cells are organized to constitute a corresponding memory trace. We established a unique imaging system that combines Ca2+ imaging and engram identification to extract the characteristics of engram activity by visualizing and discriminating between engram and non-engram cells. Here, we show that engram cells detected in the hippocampus display higher repetitive activity than non-engram cells during novel context learning. The total activity pattern of the engram cells during learning is stable across post-learning memory processing. Within a single engram population, we detected several sub-ensembles composed of neurons collectively activated during learning. Some sub-ensembles preferentially reappear during post-learning sleep, and these replayed sub-ensembles are more likely to be reactivated during retrieval. These results indicate that sub-ensembles represent distinct pieces of information, which are then orchestrated to constitute an entire memory

The 3rd DBCLS BioHackathon: improving life science data integration with Semantic Web technologies.

BACKGROUND: BioHackathon 2010 was the third in a series of meetings hosted by the Database Center for Life Sciences (DBCLS) in Tokyo, Japan. The overall goal of the BioHackathon series is to improve the quality and accessibility of life science research data on the Web by bringing together representatives from public databases, analytical tool providers, and cyber-infrastructure researchers to jointly tackle important challenges in the area of in silico biological research. RESULTS: The theme of BioHackathon 2010 was the 'Semantic Web', and all attendees gathered with the shared goal of producing Semantic Web data from their respective resources, and/or consuming or interacting those data using their tools and interfaces. We discussed on topics including guidelines for designing semantic data and interoperability of resources. We consequently developed tools and clients for analysis and visualization. CONCLUSION: We provide a meeting report from BioHackathon 2010, in which we describe the discussions, decisions, and breakthroughs made as we moved towards compliance with Semantic Web technologies - from source provider, through middleware, to the end-consumer.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

コツ ケイセイ インシ ハツゲン アデノウィルス ベクター オ モチイタ コツ ユウドウノウ ニ ツイテ

京都大学0048新制・課程博士博士(医学)甲第8820号医博第2323号新制||医||761(附属図書館)UT51-2001-F150京都大学大学院医学研究科外科系専攻(主査)教授 中村 孝志, 教授 開 祐示, 教授 飯塚 忠彦学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDA

Accelerators of Osteogenesis by Recombinant Human Bone Morphogenetic Protein-2

Bone morphogenetic protein (BMP) appears to be one of the most promising cytokine and for clinical use in reconstructive surgery for bony defects and augmentation. To evaluate the effect of basic fibroblast growth factor (bFGF), FK506, elcatonin, and hyperbaric oxygenation (HBO) on osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2), 2 or 5 μg of rhBMP-2 was implanted into intramuscular sites of rats. At 21 days after implantation, the osteoinductive activity in the treatment group and control group was compared radiographically, biochemically, and histologically. The amount of new bone in the treatment group was significantly greater than that in the control group. The alkaline phosphatase activity and calcium content in the treatment group were significantly higher than those in the control group. These results suggest that bFGF, FK506, elcatonin, and HBO accelerated the activity and rate of osteoinduction by rhBMP2. These results may be useful when BMP is applied clinically in near future

NUMERICAL ANALYSIS ON NEW DIKE BACK SLOPE SHAPE TO REDUCE SCOURING AT THE LANDWARD TOW CAUSED BY THE TSUNAMI OVERFLOW

　2011年3月11日の東日本大震災において，海岸堤防は大きく被災し，その原因は堤防の裏法尻の洗掘を始めとする裏法側での破壊であることが多く指摘されている．著者らは，津波に対する堤防の粘り強さを検討する上で，越流した際はできる限りスムーズに陸側へ流すことが有効であると考えた．そこで，数値波動水槽(CADMAS-SURF/3D)を用いた数値解析により，作用する津波高を変化させ，構造諸元の違いによる裏法面への津波作用外力の違いを明らかにし，堤防の「粘り強さ」の発生メカニズムと設計上の留意点について考察した． 　その結果，海岸堤防裏法尻部の洗掘抑制には，裏法面の緩勾配化の他，裏法肩及び法尻部に曲線形を用い法尻陸側に落堀を採用することが有効であることを確認した．Most of coastal structures, especially coastal dikes were destroyed by the 2011 Tohoku Tsunami. It was reported that one of the most important causes was the failure of back slope of dikes due to scouring.  It was effective to drain into the landside as possible smoothly in examining the robustness of the dike for the tsunami. In this study, a numerical model using CADMAS-SURF/3D has been applied to clarify the difference of tsunami external force toward back slope of dikes due to structure specification and to make suggestions for mechanism of robustness and design of coastal dikes. As a result, in order to restrain scouring at back slope of dikes, excepting method of moderating the back slope, streamline at top of inner slope and back slope were proposed