Synthesis, characterization, and evaluation of cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing effects of copper nanoparticles using the aqueous extract of Strawberry fruit and L-Ascorbic acid

© 2020 Elsevier Ltd Fragaria ananassa, also known as “Strawberry” is a common species in Iran and widely used for its anti-inflammatory, anti-ulcer, astringent, anti-allergic, antibacterial, antifungal, and antidiarrheal activities and also in the treatment of skin wounds. The purpose of the study was chemical characterization and assessment of cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing properties of copper nanoparticles (CuNPs) using the aqueous extract of Strawberry fruit and L-Ascorbic acid as reducing and stabilizing agents. These nanoparticles were characterized by FT-IR, UV–visible spectroscopy, EDS, FE-SEM, and TEM analysis. TEM images exhibited a uniform spherical morphology and diameters of 10–30 nm for the biosynthesized nanoparticles. DPPH free radical scavenging test revealed similar antioxidant properties for Strawberry, CuNPs, and butylated hydroxytoluene. The Strawberry and synthesized CuNPs had great cell viability dose-dependently against HUVEC cell line. In the microbiological part of this study, CuNPs showed higher antibacterial and antifungal properties than all standard antibiotics (p ≤ 0.01). Also, CuNPs prevented the growth of all bacteria at 2–8 mg/mL concentrations and destroyed them at 2–16 mg/mL concentrations (p ≤ 0.01). In the case of antifungal property of CuNPs, they inhibited the growth of all fungi at 2–4 mg/mL concentrations and destroyed them at 2–8 mg/mL concentrations (p ≤ 0.01). In vivo design, the use of CuNPs ointment in the treatment groups substantially remarkably raised (p ≤ 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and reduced (p ≤ 0.01) the wound area, total cells, neutrophil, macrophage, and lymphocyte compared to Strawberry, CuSO4, tetracycline, Eucerin basal, and untreated control groups. In conclusion, the results of chemical characterization confirm that the Strawberry fruit can be consumed to produce copper nanoparticles with a remarkable amount of remedial effects without any cytotoxicity against HUVECs

Prevalence of Cardiovascular Risk Factors, Reperfusion Therapy and Mortality in Patients With ST-Elevation Myocardial Infarction in Elderly and Middle-ages

Objectives The prevalence of cardiovascular risk factors is different in the elderly and middle-aged. Therefore the present study aims to compare the prevalence of risk factors affecting ST-elevation myocardial infarction (STEMI), type of treatment, and mortality rate in these two groups. Methods & Materials This retrospective cohort study included 1 071 elderly and middle-aged ST-elevation myocardial infarction (STEMI) patients who had referred to Kermanshah Imam Ali Hospital for 23 months from January 2017 to November 2019. Demographic information, cardiovascular risk factors, type of treatment, and mortality were examined. Data were analyzed with descriptive statistics, independent t test, and Chi-square test in Stata software version 14 with a significance level of 0.05. Results In the present study, the prevalence of risk factors, including smoking, high triglyceride, overweight, and obesity in the middle-aged group was significantly more than the elderly group (P < 0.05). The low-density lipoprotein (LDL) mean (mg/dL) in the middle-aged group (99.26 ± 71.69) was significantly higher than the elderly group (96.29 ± 8.5) (P < 0.05). The rate of primary angioplasty use was higher in the middle-aged (55.31%) than in the elderly (48.52%). Other results indicated that the overall mortality rate in the elderly (15.7%) was higher than in the middle-aged (4.4%) (P < 0.001). The prevalence of myocardial infarction, stroke, hypertension, hyperlipidemia, and glomerular filtration rate (GFR) < 60 was significantly higher in the elderly group compared to the middle-aged (P < 0.05). Conclusion The prevalence of low glomerular filtration rate (GFR), hyperlipidemia, and hypertension, myocardial infarction and stroke is higher in the elderly than in the middle-aged, while the prevalence of vital risk factors, such as smoking, triglycerides, overweight, and obesity in the middle-aged group is significantly higher than the elderly. If these risk factors are planned and controlled in middle age, people will be less at risk for myocardial infarction in aging