4 research outputs found
Construction and Immunological Evaluation of CpG-Au@HBc Virus-Like Nanoparticles as a Potential Vaccine
Tat/HA2 Peptides Conjugated AuNR@pNIPAAm as a Photosensitizer Carrier for Near Infrared Triggered Photodynamic Therapy
To achieve an efficiency of intracellular
photosensitizers (PSs)
delivery and efficacy of photodynamic therapy, we have developed a
novel class of PS formulation for encapsulating sulfonated aluminum
phthalocyanine (AlPcS<sub>4</sub>) by taking advantage of the membrane-disruptive
peptides Tat/HA2 and the photothermally triggered delivery system
using AuNR@pNIPAAm. The coordinated effects of cell penetrating peptide
Tat and fusogenic peptide HA2 could enhance the efficient cellular
internalization and endo/lysosome escape of PSs delivery systems.
Singlet oxygen generation was inhibited due to the reaction between
loaded AlPcS<sub>4</sub> and Au nanorods, which indicated that the
AlPcS<sub>4</sub>-loaded, AuNR@pNIPAAm delivery system might be nonphototoxic
in the circulatory system. However, this PSs-loaded nanosystem became
highly phototoxic as it underwent the near-infrared irradiation by
using the combined lights of 808 and 680 nm. Upon irradiation, the
Tat/HA2 conjugated AuNR@pNIPAAm-Pc elicited an active photodynamic
response against the cancer cells, leading to effective cells killing
via mitochondria-associated apoptotic pathway. This study also demonstrated
improved PDT therapeutic efficacy after intravenous administration
of Tat/HA2-AuNR@pNIPAAm-Pc and the subsequent lights irradiations
in tumor-bearing mice. We describe here a strategy for enhanced photodynamic
eradication of solid tumors by endo/lysosomal escape and highlight
the great promise of peptide-based nanocarriers used for cancer therapy