Association between the behavior of eating disordered and sleep disturbance in Shahid Beheshti University of Medical Students

Background: Recent studies have shown that sleep difficulties could be related to abnormal eating patterns. Apparently, eating and sleeping behaviors both affect human lives. Dysregulation of these behaviors lead to distress and negative health and psychological outcomes. The present study aimed to investigate the association between disordered eating behaviors and sleep disturbance among medical students.   Methods: This was a cross-sectional study which was conducted at Shahid Beheshti University of medical sciences. The eating disorder behaviors were assessed based on the Eating Attitudes Test-40 questionnaire and Sleep disturbances were assessed by two items related to difficulties initiating sleep (DIS) and maintaining sleep (DMS). Each individual correlation was used to calculate variable associations. Linear multiple regression analysis was performed to identify variables contributing to an explanation of sleep difficulties.     Results: In total, 172 people participated in this study. It was observed that bulimic behaviors (BB) and social pressure to eat (SPE) were the dimensions that significantly explained difficulties maintaining sleep (r=0.207, P<0.001 and r=0.286, P<0.001, respectively) and overall sleep disturbances (r=0.355, P=0.001 and r=0.225, P=0.003, respectively). Negative correlation between body mass index (BMI) and difficulties maintaining sleep (DMS) (r=-0.069, P=0.527) was not remarked which was found in males.    Conclusion: It was ultimately attained that age and sex could be accounted as confounder variables in the association between sleep disturbance and eating behaviors. The results indicated that a normal eating behavior has a positive relation with the quality of sleep

The effect of cell derived microparticles in transfusion medicine and adaptive immune system

This article reviews will focus on the concept and formation of micro particles (MPs) in circulation and their role in transfusion medicine and immune system. MPs are cell membrane derived vesicles which express markers of their parent cells and are found in circulation at low levels. Exact functions of MPs are unclear. In here, Physiological almost all types of circulating MPs including platelets MPs (PMPs), leukocytes MPs (LMPs), red blood cells MPs (RMPs) and endothelial cells MPs (EMPs) have been discussed. Furthermore, MPs present in plasma and blood products and their levels increase during storage. Thus, it can be stated that MPs are likely to cause transfusion reactions, particularly thrombotic complications and Transfusion-Related Acute Lung Injury (TRALI). Also, it is shown that the MPs may affect the immune system. However, to prove these, more and extensive studies both in vivo and in vitro need to be done

Frequency and determinants of domestic violence against Iranian women during the COVID-19 pandemic : a national cross-sectional survey

Abstract Introduction: Domestic violence (social, legal, and health violence) is the most common type of violence against women. Due to factors such as the current quarantine, this type of violence has increased during the COVID-19 pandemic. The present study aimed to assess the prevalence and risk factors for domestic violence against Iranian women during the COVID-19 pandemic. Methods: This online cross-sectional study was conducted on 203 Iranian women during May-June 2020. Data were collected using a domestic violence questionnaire, which measured three forms of violence, including physical, emotional, and sexual violence. A link of the questionnaire was distributed among anonymous subjects through social networking apps, such as WhatsApp and Telegram. The subjects were asked to complete the questionnaire based on their experiences during the COVID-19 pandemic. Data analysis was performed using descriptive statistics and a regression model. Results: The descriptive results showed that the mean domestic violence against women in all the participants was 34.9 (SD: 17.28). In addition, 26.6% (n=84), 26.1% (n=53), and 21.2% of the subjects (n=43) experienced high levels of physical, emotional, and sexual violence during the COVID-19 pandemic, respectively. The regression model also indicated that lower age, illiteracy/primary education, previous marriage(s), and unwanted/unwise marriage were the significant risk factors for domestic violence against women. Conclusion: According to the results, domestic violence against women is common among Iranian women during the COVID-19 pandemic. Therefore, strategies are urgently needed to prevent and minimize such domestic violence, and such strategies could be adopted through providing educational opportunities, raising awareness, promoting wanted/wise marriage, and providing social support and rehabilitation opportunities to vulnerable social groups, especially vulnerable women

Removal of reactive dyes from aqueous solutions by a non-conventional and low cost agricultural waste: adsorption on ash of Aloe Vera plant

Dyes are an important class of pollutants and disposal of them in precious water resources must be avoided. Among various methods adsorption occupies a prominent place in dye removal. The aim of this study is to evaluate adsorption of dye Reactive Red 198 and Blue 19 (RR-198 & RB-19 (on to Aloe Vera plant ash from aqueous solutions. In this research Aloe Vera ash was prepared at laboratory conditions and then after shredding, screened by ASTM standard sieve with 60 -200 mesh sizes and the effects of pH (3-12), adsorbent dose (0.1-1 g/L), contact time (10-60 min), initial dye concentration (10-160 mg/L) and temperature were investigated in the experiment. In different samples Dye concentration was measured by spectrophotometer at 592 nm and 520 nm wavelength for RR198 and RB19 respectively. Also the Langmuir and Freundlich adsorption isotherms were determined in order to describe the relations between the colored solutions and the adsorbent. The results of this study showed that acidic conditions were more conducive to enhance the hydrolysis rate than basic ones as the decomposition was optimum at pH 3. The adsorption rate of RR-198 and RB-19 dyes was increased by increasing of initial dye concentration, increasing of adsorbent dose in 0.1 to 0.4 mg/L. Dye solution was decolorized in a relatively short time (20 min). The efficiencies for RR-198 and RB- 19 reactive dyes were 82.68% and 90.42% respectively. The maximum adsorption capacity (qmax) has been found to be 80.152 mg/g for RR-198 reactive dye and 88.452 mg/g for Blue 19 reactive dye. Adsorption isotherms were examined by Freundlich and Langmuir isotherm that finally showed the Freundlich multilayer isotherm has better accordance with dates. The results indicate that Aloe Vera ash plant as a natural and inexpensive adsorbent is a suitable adsorbent for the adsorption of textile dyes

What if no Specific Treatment or Vaccine Develops, in the Status of COVID-19 Pandemic?

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is leading to a great number of mortalities throughout the world these days. To date, no specific drug or vaccine has been developed so the only available options are preventive strategies like quarantine. In summer, the transmission rate of this virus may reduce, however, it is possible that the disease prevails and becomes endemic

Ferroptosis as a Potential Cell Death Mechanism Against Cisplatin-Resistant Lung Cancer Cell Line

Purpose: Drug resistance is a challenging issue in cancer chemotherapy. Cell death induction is one of the main strategies to overcome chemotherapy resistance. Notably, ferroptosis has been considered a critical cell death mechanism in recent years. Accordingly, in this study, the different cell death strategies focused on ferroptosis have been utilized to overcome cisplatin resistance in an in vitro lung cancer model. Methods: The physiological functions of Akt1 and GPX4, as critical targets for ferroptosis and apoptosis induction, were suppressed by siRNA or antagonistic agents in resistant A549 cells. Afterward, the interventions’ impacts on cell viability and reactive oxygen species (ROS) amount were analyzed by flow cytometry. Moreover, the alteration in the relevant gene and protein expression levels were quantified using Real-time PCR and western blot methods. Results: The result showed that the treatment with Akt1 siRNA reversed the cisplatin resistance in the A549 cell line through the induction of apoptosis. Likewise, the combination treatment of the GPX4 siRNA or FIN56 as ferroptosis inducers alongside cisplatin elevated ROS’s cellular level, reduced the cellular antioxidant genes level and increased the cisplatin cytotoxic effect. Conclusion: In conclusion, our study indicated that ferroptosis induction can be considered a promising cell death strategy in cisplatin-resistant cancer cells

Sludge quality in wastewater treatment plant in Shokohieh industrial Park of Qom province in Iran

The general attitude of the wastewater treatment process in our country is focus on the effluent quality and unfortunately waste sludge quality is rarely considered. Since multiple natural hazards and routes the contact with sludge requires a comprehensive approach to protect public health and the environment. The aim of this study is an assessment of sludge management and finding out the potential use of sludge for land application. The study was conducted in January to December 2014. samples were gathered in four seasons, winter, spring, summer and autumn from wastewater treatment plant in industrial park of Shokohieh of Qom province according to the instructions in standard method. Sampling was moments and composite. Numbers of Samples were 24 that 2 samples were captured in every month. Samples measured base on standard method for water wastewater examination references and then data was analyzed by SPSS and t-test. The results show that the Physical Parameters qualities and nutrients qualities in sludge are good. The Phosphate quality is not suitable. Based on t-test, the results show that levels of fecal coliform and total coliform in cold and warm seasons are different (

High Resolution Melting Curve Assay for Detecting rs12979860 IL28B Polymorphisms Involved in Response of Iranian Patients to Chronic Hepatitis C Treatment

Background: A recent genome-wide association study (GWAS) on patients with chronic hepatitis C (CHC) treated with peginterferon and ribavirin (pegIFN-α/RBV) identified a single nucleotide polymorphism (SNP) on chromosome 19 (rs12979860) which was strongly associated with a sustained virological response (SVR). The aim of this study was twofold: to study the relationship between IL28B rs12979860 and sustained virological response (SVR) to pegIFN-α/RVB therapy among CHC patients and to detect the rs12979860 polymorphism by high resolution melting curve (HRM) assay as a simple, fast, sensitive, and inexpensive method. Materials and Methods: The study examined outcomes in 100 patients with chronic hepatitis C in 2 provinces of Iran from December 2011 to June 2013. Two methods were applied to detect IL28B polymorphisms: PCR-sequencing as a gold standard method and HRM as a simple, fast, sensitive, and inexpensive method. Results: The frequencies of IL28B rs12979860 CC, CT, and TT alleles in chronic hepatitis C genotype 1a patients were 10% (10/100), 35% (35/100), and 6% (6/100) and in genotype 3a were 13% (13/100), 31% (31/100), and 5% (5/100), respectively. In genotype 3a infected patients, rs12979860 (CC and CT alleles) and in genotype 1a infected patients (CC allele) were significantly associated with a sustained virological response (SVR). The SVR rates for CC, CT and TT (IL28B rs12979860) were 18%, 34% and 4%, respectively. Multiple logistic regression analysis identified two independent factors that were significantly associated with SVR: IL-28B genotype (rs 12979860 CC vs TT and CT; odds ratio [ORs], 7.86 and 4.084, respectively), and HCV subtype 1a (OR, 7.46). In the present study, an association between SVR rates and IL28B polymorphisms was observed. Conclusions: The HRM assay described herein is rapid, inexpensive, sensitive and accurate for detecting rs12979860 alleles in CHC patients. This method can be readily adopted by any molecular diagnostic laboratory with HRM capability and will be clinically beneficial in predicting treatment response in HCV genotype 1 and 3 infected patients. In addition, it was demonstrated that CC and CT alleles in HCV-3a and the CC allele in HCV-1a were significantly associated with response to pegIFN-α/RBV treatment. The present results may help identify subjects for whom the therapy might be successful

Association between Rotavirus Infection and Irritable Bowel Syndrome: A Case-control Study in Kerman - Iran

Background: Irritable bowel syndrome is a functional gastrointestinal disease of unknown etiology. Researchers have recently drawn attention to the possible role of viruses in the development of IBS and provided evidence in this regard. In this study, it was decided to investigate the possible role of rotavirus infection in the onset of IBS.Methods: Stool and serum samples were collected from 40 patients with IBS and 40 healthy individuals. To evaluate the previous exposure to rotavirus we checked the presence and concentration of anti-rotavirus IgG by ELISA. ELISA test was performed on the serum samples. Real-time PCR test was also used to measure the viral load in the stool. Finally, the data were analyzed by SPSS-22 software.Results: No significant relationship was found between anti-rotavirus IgG presence and Level in the serum of case and healthy individuals (p-value > 0.05) . Moreover, there was no significant difference between the viral genome load in the stool samples of the two groups (p-value > 0.05).Conclusion: According to the results, it seems unlikely that a link exists between rotavirus infection and the onset of irritable bowel syndrome, but the possible role of other gastrointestinal viruses, including coronavirus, remains

Effects of in-vitro application of pentoxifylline on the morphology of human spermatozoa after vitrification in asthenozoospermic patients

Cryopreservation of human spermatozoa is widely used in many assisted reproduction units to preserve male fertility [1]. Vitrification is based on the ultrarapid freezing and is routinely assayed for cryopreservation in assisted reproductive technology. Mohamed [2] showed that cryopreservation significantly affects progressive motility, viability and mitochondrial membrane potential of spermatozoa. Pentoxifylline (PX) is a phosphodiesterase considered to be a sperm movement enhancer, hyperactivation agent, inhibitor of reactive oxygen species and acrosome reaction-improving agent. The aim of our study was to evaluate the effect of in-vitro application of PX on sperm parameters and ultrastructure after vitrification. A total of 30 asthenozoospermic semen samples were selected and divided into two groups after vitrification: control (without PX) and experimental (with PX). A significant decrease in sperm motility, morphology and viability was observed post vitrification, but sperm motility was increased significantly following application of PX. On the other hand, PX did not exert any significant effect on the ultrastructure of the acrosome, plasma membrane and tail of vitrified spermatozoa