TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein

Background The tripartite motif (TRIM) family of proteins plays a key role in the developmental growth and therapeutic resistance of many tumors. However, the regulatory mechanisms and biological functions of TRIM proteins in human glioblastoma (GBM) are not yet fully understood. In this study, we focused on TRIM56, which emerged as the most differentially expressed TRIM family member with increased expression in GBM. Methods Western blot, real-time quantitative PCR (qRT-PCR), immunofluorescence (IF) and immunohistochemistry (IHC) were used to study the expression levels of TRIM56 and cIAP1 in GBM cell lines. Co-immunoprecipitation (co-IP) was used to explore the specific binding between target proteins and TRIM56. A xenograft animal model was used to verify the tumor promoting effect of TRIM56 on glioma in vivo. Results We observed elevated expression of TRIM56 in malignant gliomas and revealed that TRIM56 promoted glioma progression in vitro and in a GBM xenograft model in nude mice. Analysis of the Human Ubiquitin Array and co-IPs showed that cIAP1 is a protein downstream of TRIM56. TRIM56 deubiquitinated cIAP1, mainly through the zinc finger domain (amino acids 21–205) of TRIM56, thereby reducing the degradation of cIAP1 and thus increasing its expression. TRIM56 also showed prognostic significance in overall survival of glioma patients. Conclusions TRIM56-regulated post-translational modifications may contribute to glioma development through stabilization of cIAP1. Furthermore, TRIM56 may serve as a novel prognostic indicator and therapeutic molecular target for GBM.publishedVersio

Research on shipborne aided navigation system based on enhanced traffic environment perception

The traditional shipborne navigation system uses a two-dimensional electronic chart as a platform to integrate and control a variety of electronic navigation equipment information. It cannot intuitively restore the actual traffic environment or fundamentally improve the perception ability of the crew in poor visibility conditions. This paper proposes integrating three-dimensional simulations and real ship driving systems and presents research on "virtual-real" and "dynamic-static" ship navigation technology and equipment. In the proposed method, the hydrologic factors, topographic features, waterways, traffic dynamics, and ship driving information are effectively integrated, with a focus on achieving key advancements such as the integration of simulations and real ship driving as well as multi-source information fusion. A multi-angle, all-around, multi-level visual display for water traffic environments in poor visibility conditions is provided to enhance the ability of crews to perceive their traffic environments and thereby to provide auxiliary navigation for ships in complex weather conditions.</p

Time Budget and Diet of the Booted Eagles in the Breeding Season in Xinjiang, China

During the breeding seasons of 2010-2016, we have found seven nests of the Booted Eagle (Hieraaetus pennatus) in Xinjiang, the west of China. We used a method of focal sampling and infrared cameras to continually observe behaviors and nestlings’ growth. Nestling behaviors were different between nestling period and post-nestling period. Attendance at the nests by both adults decreased as the nestling aged. The female brooded significantly more than the male did during daylight hours (P=0.016, F= 8.38, df =1). The daily mean number of food items delivered to the nests by adults was 3.2 times/day in nestling period, and 0.96/day in post-nestling period. Seven orders of wild birds, three orders of mammals and domestic poultry were documented as prey

Three-Dimensional Audio-Magnetotelluric Imaging including Surface Topography of the Cimabanshuo Porphyry Copper Deposit, Tibet

Cimabanshuo deposit is a newly discovered porphyry copper (Cu) deposit with giant metallogenic potential, found in the western segment of the Gangdese metallogenic belt, Tibet. The average elevation of the deposit is greater than 5500 m and the terrain on which it is found is steep and complex. Therefore, it is untraversed, and the existing exploration works on it are weak. We used 59 AMT sites belonging to an array covering the main, proven mineralization zone and ore bodies of this deposit for an analysis of its underground electrical structure. Dimensionality and strike analysis revealed the apparent three-dimensional (3D) features near the Cu ore bodies. 3D inversion with topography was conducted for the AMT array data. A large range of high-resistivity anomaly (~500&ndash;2000 &Omega;m) appears beneath the proven Cu mineralization zone and ore bodies, which is interpreted as intrusive rocks with potassic alteration. Although containing chalcopyrite, it is characterized by middle&ndash;high resistivity due to a low sulfide content and poor connectivity. Moreover, a series of scattered conductors (~10&ndash;300 &Omega;m) around the Cu ore bodies are distributed in the shallow layer from near the surface to ~200 m, possibly indicating phyllic alteration containing pyritization and connected metal sulfides. The proven ore bodies of Cimabanshuo are mainly located at the junction regions between high-resistivity intrusive rocks and high-conductivity sericitization alteration zones. According to this research, the 3D inversion with topography of AMT data can visually display the 3D distribution of intrusive rocks and alteration zones beneath porphyry Cu deposits in high-elevation regions, and provides a reference for further exploration works

Three-Dimensional Audio-Magnetotelluric Imaging including Surface Topography of the Cimabanshuo Porphyry Copper Deposit, Tibet

Cimabanshuo deposit is a newly discovered porphyry copper (Cu) deposit with giant metallogenic potential, found in the western segment of the Gangdese metallogenic belt, Tibet. The average elevation of the deposit is greater than 5500 m and the terrain on which it is found is steep and complex. Therefore, it is untraversed, and the existing exploration works on it are weak. We used 59 AMT sites belonging to an array covering the main, proven mineralization zone and ore bodies of this deposit for an analysis of its underground electrical structure. Dimensionality and strike analysis revealed the apparent three-dimensional (3D) features near the Cu ore bodies. 3D inversion with topography was conducted for the AMT array data. A large range of high-resistivity anomaly (~500–2000 Ωm) appears beneath the proven Cu mineralization zone and ore bodies, which is interpreted as intrusive rocks with potassic alteration. Although containing chalcopyrite, it is characterized by middle–high resistivity due to a low sulfide content and poor connectivity. Moreover, a series of scattered conductors (~10–300 Ωm) around the Cu ore bodies are distributed in the shallow layer from near the surface to ~200 m, possibly indicating phyllic alteration containing pyritization and connected metal sulfides. The proven ore bodies of Cimabanshuo are mainly located at the junction regions between high-resistivity intrusive rocks and high-conductivity sericitization alteration zones. According to this research, the 3D inversion with topography of AMT data can visually display the 3D distribution of intrusive rocks and alteration zones beneath porphyry Cu deposits in high-elevation regions, and provides a reference for further exploration works

Three-Dimensional Lithospheric Electrical Structure beneath the Handan-Xingtai District, North China: Implications for Tectonic Control of Skarn-Iron Mineralization

In this study, we determined the lithospheric electrical structure beneath the Handan-Xingtai district and its adjacent regions using magnetotelluric sounding data. To the west of the Handan-Xingtai district, the crust and upper mantle beneath the Taihang Mountains are mainly characterized by high resistivity (>1000 Ωm, which we interpreted to be the relic cratonic lithosphere. In contrast, the lithosphere beneath the North China Plain to the east shows high-conductivity features (<100 Ωm) overall, which may indicate that it has suffered significant modifications. Additionally, other geological and geophysical studies suggested that this district was located in a significant boundary zone where the lithospheric thickness, temperature and geochemistry properties sharply changed. Combined with our resistivity model, we attributed this to the different degrees of lithospheric modification. Specifically, since the late Mesozoic, the subduction, roll-back and dehydration of the Pacific slab caused an unsteady asthenospheric flow and upwelling; therefore, the deep-derived melts and fluids concentrated within the uppermost mantle had even underplated or intruded into the crust, while this process had a negligible effect on the Taihang Mountains. Small-scale mantle convection and upwelling are likely to occur in this kind of transfer zone of lithospherice properties, leading to mantle-derived melts and fluids transporting upwardly near the surface, which was confirmed by the significantly enhanced conductivity beneath the ore district in our resistivity model. During this process, Fe derived from mantle-source magma or relic Precambrian metamorphic basement beneath the Taihang Mountains was extracted and emplaced along with the Yanshanian magmatism

A Wireless Intelligent Motion Correction System for Skating Monitoring Based on a Triboelectric Nanogenerator

Smart sport and big data have become inextricably linked with new technologies and devices to monitor sport-related information in real time. In this paper, a lightweight, portable and self-powered triboelectric nanogenerator (LPS-TENG) has been developed to monitor the frequency and force of skaters’ pedaling. Friction layers are formed of polytetrafluoroethylene (PTFE) and nylon films. Based on the triboelectric effect, LPS-TENG does not require an external power supply, and it can be used to monitor biomechanical motion independently. Under the conditions of 1 Hz and 17.19 N, the outputting voltage of LPS-TENG is stabilized at 14 V. Wireless data transmission is achieved with the help of the LPS-TENG and AD module. Visual feedback is provided by the upper computer system in the process of processing data. The wireless intelligent motion correction system is composed of an LPS-TENG, an AD module and a back-end computer. It can clearly analyze the changes between different frequencies and forces during skating. Results showed that the signal of tester’s high-frequency and great-force motion, was transmitted to the computer, and its feedback was given after analysis and processing successfully. The system may help coaches develop training methods, means and tactics to increase athletes’ performance and competitive level in athletic sport. The purpose of this study is to provide new ideas for monitoring skaters’ sport techniques, promote the use of force sensors in the monitoring of sport and develop intelligent assistant training systems

A New Oleanolic Acid Derivative against CCl4-Induced Hepatic Fibrosis in Rats

A novel hepatoprotective oleanolic acid derivative, 3-oxours-oleana-9(11), 12-dien-28-oic acid (Oxy-Di-OA), has been reported. In previous studies, we found that Oxy-Di-OA presented the anti-HBV (Hepatitis B Virus) activity (IC50 = 3.13 µg/mL). Remarkably, it is superior to lamivudine in the inhibition of the rebound of the viral replication rate. Furthermore, Oxy-Di-OA showed good performance of anti-HBV activity in vivo. Some studies showed that liver fibrosis may affiliate with HBV gene mutations. In addition, the anti-hepatic fibrosis activity of Oxy-Di-OA has not been studied. Therefore, we evaluated the protective effect of Oxy-Di-OA against carbon tetrachloride (CCl4)-induced liver injury in rats. Daily intraperitoneally administration of Oxy-Di-OA prevented the development of CCl4-induced liver fibrosis, which was evidenced by histological study and immunohistochemical analysis. The entire experimental protocol lasted nine weeks. Oxy-Di-OA significantly suppressed the increases of plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels (p &lt; 0.05). Furthermore, Oxy-Di-OA could prevent expression of transforming growth factor β1 (TGF-β1). It is worth noting that the high-dose group Oxy-Di-OA is superior to bifendate in elevating hepatic function. Compared to the model group, Oxy-Di-OA in the high-dose group and low-dose group can significantly reduce the liver and spleen indices (p &lt; 0.05). The acute toxicity test showed that LD50 and a 95% confidence interval (CIs) value of Oxy-Di-OA were 714.83 mg/kg and 639.73–798.73 mg/kg via intraperitoneal injection in mice, respectively. The LD50 value of Oxy-Di-OA exceeded 2000 mg/kg via gavage in mice. In addition, a simple and rapid high performance liquid chromatography-ultraviolet (HPLC-UV) method was developed and validated to study the pharmacokinetic characteristics of the compound. After single-dose oral administration, time to reach peak concentration of Oxy-Di-OA (Cmax = 8.18 ± 0.66 μg/mL) was 10 ± 2.19 h; the elimination half-life and area under the concentration-time curve from t = 0 to the last time of Oxy-Di-OA was 2.19 h and 90.21 μg·h/mL, respectively