Saponins and flavonoid glycosides from the leaves of Ziziphus mauritiana Lam. native of a forest area of Ivory Coast

International audienceZiziphus mauritiana Lam (Rhamnaceae) is traditionally used in the treatment of various ailments. The aim of the present study is to identify the major compounds of the methanol extract of the leaves of Ziziphus mauritiana growing in Ivory Coast. The methanol extract of this plant was purified by combining silica gel and RP18 HPLC to give an undescribed compounds, 6’’’-O-malonyl-ziziphus saponin I (1) along with nine known compounds from which two saponins, six flavonoids, and one chalcone derivate.The structures of these compounds were elucidated by analysis of 1D- and 2D-NMR spectroscopic data and mass spectrometry (HR-ESI-MS). A part from zizyphus saponin I (2), all the others compounds were isolated for the first time from the leaves of this species

Antibacterial and cytotoxic triterpenoids from the roots of Combretum racemosum

International audienceA new pentacyclic triterpenoid glucoside, together with fourteen known compounds, was isolated from the roots of Combretum racemosum. Combretaceae). The structure of the new compound was established as 28-O-β-d-glucopyranosyl-2α,3β,21β,23-tetrahydroxyolean-18-en-28-oate (1) on the basis of detailed spectroscopic data including MS, 1D, and 2D NMR. The inhibitory activity of compounds 1–15 against promyelocytic leukemia HL-60 and human erythromyeloblastoid leukemia K562 cell lines was evaluated. Compounds 11 (3-O-β-acetyl-ursolic acid), 14 (betulinic acid), and 15 (quadranoside II) exhibited significant cytotoxicity, with IC50 values of 13 to 50 μM. Among the isolated triterpenes, compounds 1, 3 (arjungenin), 5 (terminolic acid), and 11 exhibited moderate antibacterial activity against Staphylococcus aureus, Escherichia coli and Enterococcus faecalis (MICs within a range of 64 and 256 μg/mL)

Isolation and structure elucidation of cyclopeptide alkaloids from the leaves of Heisteria parvifolia

International audienceHeisteria parvifolia Sm. is prescribed in traditional medecine against numerous diseases in Côte d'Ivoire. Due to the shortcoming in scientifical knowledge of use of this species, our investigations revealed five undescribed cyclopeptide alkaloids added to one known derivative namely anorldianine. These compounds were elucidated by 1D and 2D-NMR experiments and comparison with literature data, and confirmed by HR-ESI-MS. Cytotoxic activity evaluation of these compounds against the chronic myeloid leukemia (K565) cell line exhibited an antiproliferative activity with cell growth inhibition from 13% to 46%

New Oleanane-type glycosides and secoiridoid glucoside from Aptandra zenkeri

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Triterpene glycosides from the aerial parts of Gouania longipetala

International audienceSix previously undescribed triterpenoid saponins, gouaniaside I-VI, were isolated from the aerial parts of Gouania longipetala Hemsl. (Rhamnaceae), in addition to four known triterpenes. The structure elucidation of these compounds was based on analyses of spectroscopic data including 1D- and 2D-NMR and HR-ESI-MS techniques. The inhibitory activity of isolated compounds against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines was evaluated and jujuboside I exhibited moderate cytotoxicity, with IC50 values of 13.5 and 21.0 μM, respectively. Among the isolated triterpenes, alphitolic acid exhibited moderate antibacterial activity against Staphylococcus aureus, Enterococcus faecalis and Escherichia coli (MICs 32, 64 and 128 μg/mL, respectively)

Triterpene saponins from the roots of Parkia bicolor A. Chev

International audienceFive undescribed triterpene-type saponins, parkibicolorosides A–E, a cassane-type diterpene, and a known trimethoxy benzene glucoside were isolated from the roots of Parkia bicolor A. Chev. Their structures were elucidated by different spectroscopic methods including 1D- and 2D-NMR experiments as well as HR-ESI-MS analysis. Their cytotoxic activity against the chronic myeloid leukemia (K562) cell line was evaluated. The monosaccharides saponins exhibited a moderate antiproliferative activity with IC50 ranging from 48.49 ± 0.16 to 81.66 ± 0.17 μM