The Impact of Text Conditions on Oral Reading Behaviors and Reading Comprehension of American College Chinese as a Foreign Language Learners

This study aimed to explore American college intermediate-level Chinese as a foreign language (CFL) learners’ oral reading behaviors and comprehension under different text conditions. It investigated how college CFL learners approached different orthographic texts in CFL reading. Twenty-one college intermediate-level CFL learners were asked to orally read three different versions of a Chinese text: a text consisting of (a) Chinese characters, (b) pinyin, and (c) Chinese characters with pinyin. Three texts were used in this study. To obtain data, the researcher took running records of the participants’ oral reading and asked them to answer several comprehension questions related to each text. The study’s findings indicated the interactive nature of CFL reading. It also revealed the impacts of text factors and reader factors and how they interacted to influence CFL oral reading and comprehension. The CFL oral reading protocol developed in this study can be used as a valuable tool for assessing and analyzing CFL readers’ oral reading errors and can also help determine the difficulty level of Chinese texts in CFL teaching. The findings from this study offer new insights into the reading processes and strategies employed by CFL readers under different text conditions. The study has important implications for research and teaching of Chinese as a foreign language

Precise and Rapid Validation of Candidate Gene by Allele Specific Knockout With CRISPR/Cas9 in Wild Mice

It is a tempting goal to identify causative genes underlying phenotypic differences among inbred strains of mice, which is a huge reservoir of genetic resources to understand mammalian pathophysiology. In particular, the wild-derived mouse strains harbor enormous genetic variations that have been acquired during evolutionary divergence over 100s of 1000s of years. However, validating the genetic variation in non-classical strains was extremely difficult, until the advent of CRISPR/Cas9 genome editing tools. In this study, we first describe a T cell phenotype in both wild-derived PWD/PhJ parental mice and F1 hybrids, from a cross to C57BL/6 (B6) mice, and we isolate a genetic locus on Chr2, using linkage mapping and chromosome substitution mice. Importantly, we validate the identification of the functional gene controlling this T cell phenotype, Cd44, by allele specific knockout of the PWD copy, leaving the B6 copy completely intact. Our experiments using F1 mice with a dominant phenotype, allowed rapid validation of candidate genes by designing sgRNA PAM sequences that only target the DNA of the PWD genome. We obtained 10 animals derived from B6 eggs fertilized with PWD sperm cells which were subjected to microinjection of CRISPR/Cas9 gene targeting machinery. In the newborns of F1 hybrids, 80% (n = 10) had allele specific knockout of the candidate gene Cd44 of PWD origin, and no mice showed mistargeting of the B6 copy. In the resultant allele-specific knockout F1 mice, we observe full recovery of T cell phenotype. Therefore, our study provided a precise and rapid approach to functionally validate genes that could facilitate gene discovery in classic mouse genetics. More importantly, as we succeeded in genetic manipulation of mice, allele specific knockout could provide the possibility to inactivate disease alleles while keeping the normal allele of the gene intact in human cells

十二生肖赛蹴鞠

Paper cutting – Chinese intangible cultural heritage. The zodiac – Chinese year. Kick the ball – the most popular sport in ancient times

Mimic Encryption Box for Network Multimedia Data Security

With the rapid development of the Internet, the security of network multimedia data has attracted increasingly more attention. The moving target defense (MTD) and cyber mimic defense (CMD) approaches provide a new way to solve this problem. To enhance the security of network multimedia data, this paper proposes a mimic encryption box for network multimedia data security. The mimic encryption box can directly access the network where the multimedia device is located, automatically complete the negotiation, provide safe and convenient encryption services, and effectively prevent network attacks. According to the principles of dynamization, diversification, and randomization, the mimic encryption box uses a reconfigurable encryption algorithm to encrypt network data and uses IP address hopping, port number hopping, protocol camouflage, and network channel change to increase the attack threshold. Second, the mimic encryption box has a built-in pseudorandom number generator and key management system, which can generate an initial random key and update the key with the hash value of the data packet to achieve “one packet, one key.” Finally, through the cooperation of the ARM and the FPGA, an access control list can be used to filter illegal data and monitor the working status of the system in real time. If an abnormality is found, the feedback reconstruction mechanism is used to “clean” the FPGA to make it work normally again. The experimental results and analysis show that the mimic encryption box designed in this paper has high network encryption performance and can effectively prevent data leakage. At the same time, it provides a mimic security defense mechanism at multiple levels, which can effectively resist a variety of network attacks and has high security

Effects of Fine Gangue on Strength, Resistivity, and Microscopic Properties of Cemented Coal Gangue Backfill for Coal Mining

The cemented coal gangue backfill (CGB) in coal mining is normally made of gangue (particle size of 0–15 mm), fly ash, cement, and water. In this study, the effects of the weight content (ranging from 20% to 60%) of fine gangue (0–5 mm) on the microscopic characteristics, resistivity, and compressive strength of CGB were investigated at 3 d and 28 d curing times, respectively. The test results indicate that the strengths of the CGB, regardless of the curing time, increased with fine gangue content changing from 20% up to 40%. Further increase in fine gangue introduced a decrease in the strength. Another observation is that, at 3 days, a general increasing trend of CGB resistivity was noted with fine gangue content. At 28 days, the resistivity of CGB decreased with increase in the fine gangue content. Correlations between the resistivity and compression strength of CGB show a concave pattern, which attribute to the various micromechanism influenced on the resistivity and strength of CGB with different fine gangue content. It indicates that using resistivity to derive strength of CGB is not appropriate

A Comparative Study of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins in Breast Cancer Cells

Our previous studies demonstrated that arming vaccinia viruses with marine lectins enhanced the antitumor efficacy in several cancer cells. This study aims to compare the efficacy of oncolytic vaccinia viruses harboring Tachypleus tridentatus lectin (oncoVV-TTL), Aphrocallistes vastus lectin (oncoVV-AVL), white-spotted charr lectin (oncoVV-WCL), and Asterina pectinifera lectin (oncoVV-APL) in breast cancer cells (BC). These results indicated that oncoVV-AVL elicited the highest anti-tumor effect, followed by oncoVV-APL, while oncoVV-TTL and oncoVV-WCL had lower effects in BC. Further studies showed that apoptosis and replication may work together to enhance the cytotoxicity of oncoVV-lectins in a cell-type dependent manner. TTL/AVL/APL/WCL may mediate multiple pathways, including ERK, JNK, Hippo, and PI3K pathways, to promote oncoVV replication in MDA-MB-231 cells. In contrast, these pathways did not affect oncoVV-TTL/AVL/APL/WCL replication in MCF-7 cells, suggesting that the mechanisms of recombinant viruses in MCF-7 (ER+, PR+) and MDA-MB-231 (TNBC) cells were significantly different. Based on this study, we hypothesized that ER or PR may be responsible for the differences in promoting viral replication and inducing apoptosis between MCF-7 and MDA-MB-231 cells, but the specific mechanism needs to be further explored. In addition, small-molecule drugs targeting key cellular signaling pathways, including MAPK, PI3K/Akt, and Hippo, could be conjunction with oncoVV-AVL to promote breast cancer therapy, and key pathway factors in the JNK and PI3K pathways may be related to the efficacy of oncoVV-APL/TTL/WCL. This study provides a basis for applying oncolytic vaccinia virus in breast carcinoma

The immunosuppression effects of deforolimus (ridaforolimus, AP23573) on allograft organ transplantation

Abstract Objects We conducted this study to confirm the immunosuppression effect of Deforolimus in heart allotransplantation and find its mechanism. Material and methods We used vitro methods to confirm the immunosuppression of Deforolimus in T cell subgroups. Then, we conducted heart allotransplantation from BALB/c donors to C57BL/6 recipients with the oral administration of Deforolimus or blank solvent to contrast the immunosuppression effect in vivo. We used the flow cytometry（）, clone anergy test, ELISA (enzyme‐linked immunosorbent assay), apoptosis test, and lymphocyte translation (LT) to find the mechanism of Deforolimus’ immunosuppressive action. Results Studies in vitro demonstrated that Deforolimus had immunosuppressive action. Studies in vivo demonstrated that Deforolimus could inhibit the immune system of the heart allotransplantation recipients to extend the recipients’ life. The mechanisms of Deforolimus’ immunosuppressive action included induction of Tregs (regulatory T cells), induction of T cells anergy, and decreasing proportion of T cells in spleens and lymph nodes. Conclusion Deforolimus suppressed the immunological rejection of mouse cardiac allotransplantation which laid the foundation of Deforolimus applying to solid organ allotransplantation